Overproduction of the extracellular lipase is closely related to that of metalloprotease in Serratia marcescens

Journal of Fermentation and Bioengineering

Volumn 81, Issue 2, 1996, Pages 115-120

  Akatsuka, Hiroyuki ^a   Kawai, Eri ^a   Omori, Kenji ^a   Komatsubara, Saburo ^a   Shibatani, Takeji ^a  

^a MITSUBISHI TANABE PHARMA CORPORATION   (Japan)

Author keywords

lipase; overproduction; secretion mechanism; Serratia marcescens

Indexed keywords

METALLOPROTEINASE; TRIACYLGLYCEROL LIPASE;

ARTICLE; CLONING; COMPETITIVE INHIBITION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME RELEASE; ENZYME SYNTHESIS; GENE EXPRESSION; GENETIC DISORDER; NONHUMAN; PLASMID; SERRATIA MARCESCENS;

SERRATIA MARCESCENS;

EID: 0029993597     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0922-338X(96)87587-8     Document Type: Article

References (50)

1. Matsumae, H. and Shibatani, T.: Purification and characterization of the lipase from Serratia marcescens Sr41 8000 responsible for asymmetric hydrolysis of 3-phenylglycidic acid esters. J. Ferment. Bioeng., 77, 152-158 (1994).
2. Matsumae, H., Furui, M., and Shibatani, T.: Lipase-catalyzed asymmetric hydrolysis of 3-phenylglycidic acid ester, the key intermediate in the synthesis of diltiazem hydrochloride. J. Ferment. Bioeng., 75, 93-98 (1993).
3. Akatsuka, H., Kawai, E., Omori, K., Komatsubara, S., Shibatani, T., and Tosa, T.: The lipA gene of Serratia marcescens which encodes an extracellular lipase having no N-terminal signal peptide. J. Bacteriol., 176, 1949-1956 (1994).
4. Pugsley, A. P., d'Enfert, C., Reyss, I., and Kornacker, M. G.: Genetics of extracellular protein secretion by gram-negative bacteria. Annu. Rev. Genet., 24, 67-90 (1990).
5. Wandersman, C.: Secretion across the bacterial outer membrane. Trends Genet., 8, 317-321 (1992).
6. Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I. S., Hase, T., and Matsubara, H.: Cloning and sequencing of Serratia protease gene. Nucleic Acids Res., 14, 5843-5855 (1986).
7. Delepelaire, P. and Wandersman, C.: Protease secretion by Erwinia chrysanthemi. J. Biol. Chem., 264, 9083-9089 (1989).
8. Delepelaire, P. and Wandersman, C.: Protease secretion in gram-negative bacteria. J. Biol. Chem., 265, 17118-17125 (1990).
9. Felmlee, T., Pellett, S., and Welch, R. A.: Nucleotide sequence of an Escherichia coli chromosomal hemolysin. J. Bacteriol., 163, 94-105 (1985).
10. Lo, R. Y. C., Strathdee, C. A., and Shewe, P. E.: Nucleotide sequence of the leukotoxin genes of Pasteurella haemolytica A1. Infect. Immun., 55, 1987-1996 (1987).
11. Glaser, P., Sakamoto, H., Bellalou, J., Ullmann, A., and Danchin, A.: Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of Bordetella pertussis. EMBO J., 7, 3997-4004 (1988).
12. 2+-binding protein that is exported without N-terminal cleavage and is homologous to haemolysin and related proteins. EMBO J., 9, 349-354 (1990).
13. Gilson, L., Mahanty, H. K., and Kolter, R.: Genetic analysis of an MDR-like export system: the secretion of colicinV. EMBO J., 9, 3875-3884 (1990).
14. 2+-dependent binding to erythrocytes. Mol. Gen. Genet., 214, 553-561 (1988).
15. Blight, M. A. and Holland, I. B.: Structure and function of haemolysin B, P-glycoprotein and other members of a novel family of membrane translocators. Mol. Microbiol., 4, 873-880 (1990).
16. Mackman, N., Nicaud, J.-M., Gray, L., and Holland, I. B.: Secretion of haemolysin by Escherichia coli. Curr. Top. Microb. Immunol., 125, 159-181 (1986).
17. Wagner, W., Vogel, M., and Goebel, W.: Transport of hemolysin across the outer membrane of Escherichia coli requires two functions. J. Bacteriol., 154, 200-210 (1983).
18. Wandersman, C. and Delepelaire, P.: TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc. Natl. Acad. Sci. USA, 87, 4776-4780 (1990).
19. Léoffé, S., Delepelaire, P., and Wandersman, C.: Protease secretion by Envinia chrysanthemi: the specific secretion functions are analogous to those of Escherichia coli α-haemolysin. EMBO J., 9, 1375-1382 (1990).
20. Chang, Y.-F., Young, R., and Struck, D. K.: Secretion of the Pasteurella leukotoxin by Escherichia coli. FEMS Microbiol. Lett., 60, 169-174 (1989).
21. Fath, M. J., Skvirsky, R. C., and Kolter, R.: Functional complementation between bacterial MDR-like export systems: colicin V, α-hemolysin, and Erwinia protease. J. Bacteriol., 173, 7549-7556 (1991).
22. Létoffé, S., Delepelaire, P., and Wandersman, C.: Cloning and expression in Escherichia coli of the Serratia marcescens metalloprotease gene: secretion of the protease from E. coli in the presence of the Erwinia chrysanthemi protease secretion functions. J. Bacteriol., 173, 2160-2166 (1991).
23. Masure, H. R., Au, D. C., Gross, M. K., Donovan, M. G., and Storm, D. R.: Secretion of the Bordetella pertussis adenylate cyclase from Escherichia coli containing the hemolysin operon. Biochemistry, 29, 140-145 (1990).
24. Scheu, A. K., Economou, A., Hong, G. F., Ghelani, S., Johnston, A. W. B., and Downie, J. A.: Secretion of the Rhizobium leguminosarum nodulation protein NodO by haemolysin-type systems. Mol. Microbiol., 6, 231-238 (1992).
25. Guzzo, J., Duong, F., Wandersman, C., Murgier, M., and Lazdunsk, A.: The secretion genes of Pseudomonas aeruginosa alkaline protease are functionally related to those of Erwinia chrysanthemi proteases and Escherichia coli α-haemolysin. Mol. Microbiol., 5, 447-453 (1991).
26. Strathdee, C. A. and Lo, R. Y. C.: Cloning, nucleotide sequence, and characterization of genes encoding the secretion function of the Pasteurella haemolytica leukotoxin determinant. J. Bacteriol., 171, 916-928 (1989).
27. Létoffé, S., Ghigo, J. M., and Wandersman, C.: Iron acquisition from heme and hemoglobin by a Serratia marcescens extracellular protein. Proc. Natl. Acad. Sci. USA, 91, 9876-9880 (1994).
28. Létoffé, S., Ghigo, J. M., and Wandersman, C.: Identification of two components of Serratia marcescens metalloprotease transporter: protease SM secretion in Escherichia coli in TolC dependent. J. Bacteriol., 175, 7321-7328 (1993).
29. Létoffé, S., Ghigo, J. M., and Wandersman, C.: Secretion of the Serratia marcescens HasA protein by an ABC transporter. J. Bacteriol., 176, 5372-5377 (1994).
30. Sambrook, J., Fritsch, E. F., and Maniatis, T.: Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1989).
31. Yanisch-Perron, C., Vieira, J., and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33, 103-119 (1985).
32. Matsumoto, H., Hosogaya, S., Suzuki, K., and Tazaki, T.: Arginine gene cluster of Serratia marcescens, Jpn. J. Microbiol., 19, 35-44 (1975).
33. Takagi, T. and Kisumi, M.: Isolation of a versatile Serratia marcescens mutant as a host and molecular cloning of the aspartase gene. J. Bacteriol., 161, 1-6 (1987).
34. Takeshita, S., Sato, M., Toba, M., Masahashi, W., and Hashimoto-Gotoh, T.: High-copy-number plasmid vectors for lacZ α-complementation and chloramphenicol- or kanamycin-resistant selection. Gene, 61, 63-74 (1987).
35. Yamaguchi, K. and Masamune, Y.: Autogenous regulation of synthesis of the replication protein in plasmid pSC101. Mol. Gen. Genet., 200, 362-367 (1985).
36. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heynecker, H. L., W. Boyer, H., Crosa, J. H., and Falkow, S.: Construction of characterization of new cloning vehicles. II. A multipurpose cloning system. Gene, 2, 95-113 (1977).
37. Omori, K., Akatsuka, H., and Komatsubara, S.: Versatile Escherichia coli expression vectors for production of truncated proteins. Plasmid, 31, 297-299 (1994).
38. Omori, K., Suzuki, S., Imai, Y., and Komatsubara, S.: Nucleotide sequence of the Serratia marcescens threonine operon and analysis of the threonine operon mutations which alter feedback inhibition of both aspartokinase I and homoserine dehydrogenase I. J. Bacteriol., 175, 785-794 (1993).
39. Bagdasarian, M. M., Amann, E., Lurz, R., Ruckert, B., and Bagdasarian, M.: Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene, 26, 273-282 (1983).
40. Davis, R. W., Bostein, D., and R. Roth, J.: Advanced bacterial genetics: a manual for genetic engineering. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1980).
41. Hanahan, D.: Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol., 166, 557-580 (1983).
42. Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London), 228, 680-685 (1970).
43. Kurooka, S., Okamoto, S., and Hashimoto, M.: A novel and simple colorimetric assay for human serum lipase. J. Biochem., 81, 361-369 (1977).
44. Miyata, K., Maejima, K., Tomoda, K., and Isono, M.: Serratia protease. I. Purification and general properties of the enzyme. Agric Biol. Chem., 34, 310-318 (1970).
45. Higuchi, R.: Recombinant PCR, p. 117-183. In Innis, M. A., Gelfand, D. H., Sninsky, J. J., and White, T. J. (ed.), PCR protocol: a guide to methods and applications. Academic Press, San Diego (1990).
46. Kiel, J. A. K. W., Vosson, J. P. M. J., and Venema, G.: A general method for the construction of Escherichia coli mutants by homologous recombination and plasmid segregation. Mol. Gen. Genet., 207, 294-301 (1987).
47. Harlow, E. and Lane, D.: Antibody: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York (1988).
48. Yanagida, N., Uozumi, T., and Beppu, T.: Specific excretion of Serratia marcescens protease through the outer membrane of Escherichia coli. J. Bacteriol., 166, 937-944 (1986).
49. Akatsuka, H., Kawai, E., Omori, K., and Shibatani, T.: Divergence of a flagellar protein in Serratia marcescens. Gene, 163, 157-158 (1995).
50. Homma, M., Fujita, H., Yamaguchi, S., and Iino, T.: Regions of Salmonella typhimurium flagellin essential for its polymerization and secretion. J. Bacteriol., 169, 190-192 (1987).