In vitro (ex vivo) expansion of murine hematopoietic stem cells. Running title: In vitro expansion of stem cells

Vox Sanguinis

Volumn 70, Issue SUPPL. 3, 1996, Pages 68-70

  Ogawa, Makio ^a   Yonemura, Yuji ^a   Ku, Hsun ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Cytokines; Hematopoietic growth factors; Hematopoietic stem cells; Suspension culture

Indexed keywords

CYTOKINE; INTERLEUKIN 1; INTERLEUKIN 3;

CELL CULTURE; CELL TRANSPLANTATION; CONFERENCE PAPER; DISEASE SEVERITY; GRAFT VERSUS HOST REACTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; LYMPHOPOIESIS; PRIORITY JOURNAL; STEM CELL; UMBILICAL CORD BLOOD;

EID: 0029993159     PISSN: 00429007     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1423-0410.1996.tb01372.x     Document Type: Conference Paper

References (9)

1. - WGA+ cells in serum-free cultures containing steel factor, interleukin-6, and erythropoietin with maintenance of cells with long-term in vivo reconstituting potential. Blood 83: 128, 1994.
2. Knobel KM, McNally MA, Berson AE, Rood D, Chen K, Kilinski L, Tran K, Okarma TB, Lebkowski JS: Long-term reconstitution of mice after ex vivo expansion of bone marrow cells: Differential activity of cultured bone marrow and enriched stem cells populations. Exp Hematol 22: 1227, 1994.
3. Peters SO, Kittler ELW, Ramshaw HS, Quesenberry PJ: Murine marrow cells expanded in culture with IL-3, IL-6, IL-11, and SCF acquire an engraftment defect in normal hosts. Exp Hematol 23: 461, 1995.
4. Ogawa M: Differentiation and proliferation of hematopoietic stem cells. Blood 81: 2844, 1993.
5. Hirayama F, Shih JP, Awgulewitsch A, Warr GW, Clark SC, Ogawa M: Clonal proliferation of murine lymphohemopoietic progenitors in culture. Proc Natl Acad Sci USA 89: 5907, 1992.
6. Hirayama F, Lyman SD, Clark SC, Ogawa M: The FLT3 ligand supports proliferation of lymphohemopoietic progenitors and early B-lymphoid progenitors. Blood 85: 1762, 1995.
7. Hirayama F, Clark SC, Ogawa M: Negative regulation of early B-lymphopoiesis by interleukin-3 and interleukin-1a. Proc Natl Acad Sci USA 91 : 469, 1994.
8. Hirayama F, Ogawa M: Negative regulation of early T-lymphopoiesis by interleukin-3 and interleukin-1a. Blood 86: 4527, 1995.
9. Yonemura Y, Ku H, Hirayama F, Souza LM, Ogawa M: Interleukin-3 or interleukin-1 abrogates the reconstituting ability of hematopoietic stem cells. Proc Natil Aca Sci USA, in press.