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Volumn 142, Issue 7, 1996, Pages 1617-1624

Mutagenesis of three surface-exposed loops of a Bacillus thuringiensis insecticidal toxin reveals residues important for toxicity, receptor recognition and possibly membrane insertion

Author keywords

Bacillus thuringiensis endotoxin; Competition binding assay; Site directed mutagenesis

Indexed keywords

ENDOTOXIN; INSECTICIDE; METHIONINE; SULFUR 35;

EID: 0029993089     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-142-7-1617     Document Type: Article
Times cited : (55)

References (46)
  • 1
    • 0025138436 scopus 로고
    • Directed mutagenesis of selected regions of a Bacillus thuringiensis entomocidal protein
    • Ahmad, W. & Ellar, D. J. (1990). Directed mutagenesis of selected regions of a Bacillus thuringiensis entomocidal protein. FEMS Microbiol Lett 68, 97-104.
    • (1990) FEMS Microbiol Lett , vol.68 , pp. 97-104
    • Ahmad, W.1    Ellar, D.J.2
  • 2
    • 0027328978 scopus 로고
    • Structural stability of Bacillus thuringiensis δ-endotoxin homolog-scanning mutants determined by susceptibility to protease
    • Almond, B. D. & Dean, D. H. (1993). Structural stability of Bacillus thuringiensis δ-endotoxin homolog-scanning mutants determined by susceptibility to protease. Appl Environ Microbiol 59, 2442-2448.
    • (1993) Appl Environ Microbiol , vol.59 , pp. 2442-2448
    • Almond, B.D.1    Dean, D.H.2
  • 3
    • 0029048940 scopus 로고
    • Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene
    • Aronson, A. I., Wu, D. & Zhang, C. (1995). Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene. J Bacteriol 177, 4059-4065.
    • (1995) J Bacteriol , vol.177 , pp. 4059-4065
    • Aronson, A.I.1    Wu, D.2    Zhang, C.3
  • 4
    • 0024600802 scopus 로고
    • Transformation of Bacillus thuringiensis by electroporation
    • Bone, E. J. & Ellar, D. J. (1989). Transformation of Bacillus thuringiensis by electroporation. FEMS Microbiol Lett 58, 171-178.
    • (1989) FEMS Microbiol Lett , vol.58 , pp. 171-178
    • Bone, E.J.1    Ellar, D.J.2
  • 5
    • 0025969881 scopus 로고
    • Suggestions for 'safe' residue substitutions in site-directed mutagenesis
    • Bordo, D. & Argos, P. (1991). Suggestions for 'safe' residue substitutions in site-directed mutagenesis. J Mol Biol 217, 721-729.
    • (1991) J Mol Biol , vol.217 , pp. 721-729
    • Bordo, D.1    Argos, P.2
  • 6
    • 0027314529 scopus 로고
    • An analysis of Bacillus thuringiensis δ-endotoxin action on insect-midgut-membrane permeability using a light-scattering assay
    • Carroll, J. & Ellar, D. J. (1993). An analysis of Bacillus thuringiensis δ-endotoxin action on insect-midgut-membrane permeability using a light-scattering assay. Eur J Biochem 214, 771-778.
    • (1993) Eur J Biochem , vol.214 , pp. 771-778
    • Carroll, J.1    Ellar, D.J.2
  • 8
    • 0027945092 scopus 로고
    • Chemical modification of Bacillus thuringiensis activated δ-endotoxin and its effect on toxicity and binding to Manduca sexta midgut membranes
    • Cummings, C. E. & Ellar, D. J. (1994). Chemical modification of Bacillus thuringiensis activated δ-endotoxin and its effect on toxicity and binding to Manduca sexta midgut membranes. Microbiology 140, 2737-2747.
    • (1994) Microbiology , vol.140 , pp. 2737-2747
    • Cummings, C.E.1    Ellar, D.J.2
  • 9
    • 84910444026 scopus 로고
    • Characterisation of forespores isolated from Bacillus megaterium at different stages of development into mature spores
    • Edited by A. N. Barker, G. W. Gould & J. Wolf. London: Academic Press
    • Ellar, D. J. & Posgate, J. A. (1974). Characterisation of forespores isolated from Bacillus megaterium at different stages of development into mature spores. In Spore Research 1973, pp. 21-40. Edited by A. N. Barker, G. W. Gould & J. Wolf. London: Academic Press.
    • (1974) Spore Research 1973 , pp. 21-40
    • Ellar, D.J.1    Posgate, J.A.2
  • 10
    • 0004116672 scopus 로고
    • Edited by D. J. Finney. Cambridge: Cambridge University Press
    • Finney, D. J. (1952). Probit Analysis, 2nd edn. Edited by D. J. Finney. Cambridge: Cambridge University Press.
    • (1952) Probit Analysis, 2nd Edn.
    • Finney, D.J.1
  • 11
    • 0025948489 scopus 로고
    • Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis
    • Garczynski, L. F., Crim, J. W. & Adang, M. J. (1991). Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis δ-endotoxin by protein blot analysis. Appl Environ Microbiol 57, 2816-2820.
    • (1991) Appl Environ Microbiol , vol.57 , pp. 2816-2820
    • Garczynski, L.F.1    Crim, J.W.2    Adang, M.J.3
  • 12
    • 0027190573 scopus 로고
    • Structural and functional characterisation of the α5 segment of Bacillus thuringiensis δ-endotoxin
    • Gazit, E. & Shai, Y. (1993). Structural and functional characterisation of the α5 segment of Bacillus thuringiensis δ-endotoxin. Biochemistry 32, 3429-3436.
    • (1993) Biochemistry , vol.32 , pp. 3429-3436
    • Gazit, E.1    Shai, Y.2
  • 13
    • 0024687823 scopus 로고
    • Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein
    • Ge, A. Z., Shivarova, N. I. & Dean, D. H. (1989). Location of the Bombyx mori specificity domain on a Bacillus thuringiensis delta-endotoxin protein. Proc Natl Acad Sci USA 86, 4037-4041.
    • (1989) Proc Natl Acad Sci USA , vol.86 , pp. 4037-4041
    • Ge, A.Z.1    Shivarova, N.I.2    Dean, D.H.3
  • 14
    • 0025949194 scopus 로고
    • Functional domains of Bacillus thuringiensis insecticidal crystal proteins: Refinement of Heliothis virescens and Trichoplusia ni specificity domains on CryIAc
    • Ge, A. Z., Rivers, D., Milne, R. & Dean, D. H. (1991). Functional domains of Bacillus thuringiensis insecticidal crystal proteins: refinement of Heliothis virescens and Trichoplusia ni specificity domains on CryIAc. J Biol Chem 266, 17954-17958.
    • (1991) J Biol Chem , vol.266 , pp. 17954-17958
    • Ge, A.Z.1    Rivers, D.2    Milne, R.3    Dean, D.H.4
  • 15
    • 0024278416 scopus 로고
    • Binding of the delta-endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae)
    • Hofmann, C., Lüthy, P., Hutter, R. & Pliska, V. (1988a). Binding of the delta-endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae). Eur J Biochem 173, 85-91.
    • (1988) Eur J Biochem , vol.173 , pp. 85-91
    • Hofmann, C.1    Lüthy, P.2    Hutter, R.3    Pliska, V.4
  • 16
    • 0006952717 scopus 로고
    • Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts
    • Hofmann, C., Vanderbruggen, H., Hofte, H., Van Rie, J., Jansens, S. & Van Mellaert, H. (1988b). Specificity of Bacillus thuringiensis δ-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts. Proc Natl Acad Sci USA 85, 7844-7848.
    • (1988) Proc Natl Acad Sci USA , vol.85 , pp. 7844-7848
    • Hofmann, C.1    Vanderbruggen, H.2    Hofte, H.3    Van Rie, J.4    Jansens, S.5    Van Mellaert, H.6
  • 17
    • 0024337104 scopus 로고
    • Insecticidal crystal proteins of Bacillus thuringiensis
    • Hofte, H. & Whiteley, H. R. (1989). Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53, 242-255.
    • (1989) Microbiol Rev , vol.53 , pp. 242-255
    • Hofte, H.1    Whiteley, H.R.2
  • 18
    • 0025728840 scopus 로고
    • A fast and simple procedure for sequencing double stranded DNA with Sequenase
    • Hsaio, K. C. (1991). A fast and simple procedure for sequencing double stranded DNA with Sequenase. Nucleic Acids Res 19, 2797.
    • (1991) Nucleic Acids Res , vol.19 , pp. 2797
    • Hsaio, K.C.1
  • 19
    • 0002042303 scopus 로고
    • Bacillus thuringiensis delta endotoxin: Composition and activation
    • Edited by E. D. Davidson. Totowa, NJ: Allanheld, Osmum & Co.
    • Huber, H. E. & Lüthy, P. (1981). Bacillus thuringiensis delta endotoxin: composition and activation. In Pathogenesis of Invertebrate Microbial Diseases, pp. 209-234. Edited by E. D. Davidson. Totowa, NJ: Allanheld, Osmum & Co.
    • (1981) Pathogenesis of Invertebrate Microbial Diseases , pp. 209-234
    • Huber, H.E.1    Lüthy, P.2
  • 20
    • 0028291484 scopus 로고
    • The receptor for Bacillus thuringiensis CryIA(c) delta-endotoxin in the brush border membrane of the lepidopteran M. sexta is aminopeptidase N
    • Knight, P. J. K., Crickmore, N. & Ellar, D. J. (1994). The receptor for Bacillus thuringiensis CryIA(c) delta-endotoxin in the brush border membrane of the lepidopteran M. sexta is aminopeptidase N. Mol Microbiol 11, 429-436.
    • (1994) Mol Microbiol , vol.11 , pp. 429-436
    • Knight, P.J.K.1    Crickmore, N.2    Ellar, D.J.3
  • 21
    • 0029027032 scopus 로고
    • Molecular cloning of an insect aminopeptidase N that serves as a receptor for Bacillus thuringiensis CryIA(C) toxin
    • Knight, P. J. K., Knowles, B. H. & Ellar, D. J. (1995). Molecular cloning of an insect aminopeptidase N that serves as a receptor for Bacillus thuringiensis CryIA(C) toxin. J Biol Chem 270, 17765-17770.
    • (1995) J Biol Chem , vol.270 , pp. 17765-17770
    • Knight, P.J.K.1    Knowles, B.H.2    Ellar, D.J.3
  • 22
    • 0023183759 scopus 로고
    • Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different specificity
    • Knowles, B. H. & Ellar, D. J. (1987). Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different specificity. Biochim Biophys Acta 924, 509-518.
    • (1987) Biochim Biophys Acta , vol.924 , pp. 509-518
    • Knowles, B.H.1    Ellar, D.J.2
  • 23
    • 0000035290 scopus 로고
    • Lectin-like binding of Bacillus thuringiensis var. kurstaki lepidopteran-specific toxin is an initial step in insecticidal action
    • Knowles, B. H., Thomas, W. E. & Ellar, D. J. (1984). Lectin-like binding of Bacillus thuringiensis var. kurstaki lepidopteran-specific toxin is an initial step in insecticidal action. FEBS Lett 168, 197-202.
    • (1984) FEBS Lett , vol.168 , pp. 197-202
    • Knowles, B.H.1    Thomas, W.E.2    Ellar, D.J.3
  • 24
    • 0025871617 scopus 로고
    • N-Acetylgalactosamine is part of the receptor in insect gut epithelia that recognises an insecticidal protein from Bacillus thuringiensis
    • Knowles, B. H., Knight, P. J. K. & Ellar, D. J. (1991). N-Acetylgalactosamine is part of the receptor in insect gut epithelia that recognises an insecticidal protein from Bacillus thuringiensis. Proc R Soc Lond B 245, 31-35.
    • (1991) Proc R Soc Lond B , vol.245 , pp. 31-35
    • Knowles, B.H.1    Knight, P.J.K.2    Ellar, D.J.3
  • 25
    • 0026684615 scopus 로고
    • Characterisation of the pH-mediated solubility of Bacillus thuringiensis var. san diego native δ-endotoxin crystals
    • Koller, C. N., Bauer, L. S. & Hollingworth, R. M. (1992). Characterisation of the pH-mediated solubility of Bacillus thuringiensis var. san diego native δ-endotoxin crystals. Biochem Biophys Res Commun 184, 692-699.
    • (1992) Biochem Biophys Res Commun , vol.184 , pp. 692-699
    • Koller, C.N.1    Bauer, L.S.2    Hollingworth, R.M.3
  • 26
    • 0015853344 scopus 로고
    • Maturation of the head of bacteriophage T4
    • Laemmli, U. K. & Favre, M. (1973). Maturation of the head of bacteriophage T4. J Mol Biol 80, 575-599.
    • (1973) J Mol Biol , vol.80 , pp. 575-599
    • Laemmli, U.K.1    Favre, M.2
  • 27
    • 0026760525 scopus 로고
    • Location of Bombyx mori receptor binding region of a Bacillus thuringiensis delta-endotoxin
    • Lee, M. K., Milne, R. E., Ge, A. Z. & Dean, D. H. (1992). Location of Bombyx mori receptor binding region of a Bacillus thuringiensis delta-endotoxin. J Biol Chem 267, 3115-3121.
    • (1992) J Biol Chem , vol.267 , pp. 3115-3121
    • Lee, M.K.1    Milne, R.E.2    Ge, A.Z.3    Dean, D.H.4
  • 28
    • 0026050639 scopus 로고
    • Crystal structure of insecticidal δ-endotoxin at 2.4 Å resolution
    • Li, J., Carroll, J. & Ellar, D. J. (1991). Crystal structure of insecticidal δ-endotoxin at 2.4 Å resolution. Nature 353, 815-821.
    • (1991) Nature , vol.353 , pp. 815-821
    • Li, J.1    Carroll, J.2    Ellar, D.J.3
  • 29
    • 0020646341 scopus 로고
    • 50 using the probit technique: A basic computer program
    • 50 using the probit technique: a basic computer program. Drug Chem Toxicol 6, 111-116.
    • (1983) Drug Chem Toxicol , vol.6 , pp. 111-116
    • Lieberman, H.R.1
  • 30
    • 0028128867 scopus 로고
    • Identification of amino acid residues of Bacillus thuringiensis δ-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori
    • Lu, H., Rajamohan, F. & Dean, D. H. (1994). Identification of amino acid residues of Bacillus thuringiensis δ-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. J Bacteriol 176, 5554-5559.
    • (1994) J Bacteriol , vol.176 , pp. 5554-5559
    • Lu, H.1    Rajamohan, F.2    Dean, D.H.3
  • 31
    • 0019061918 scopus 로고
    • Data analysis and curve fitting for ligand binding experiments
    • Munson, P. J. & Rodbard, D. (1980). Data analysis and curve fitting for ligand binding experiments. Anal Biochem 107, 220-239.
    • (1980) Anal Biochem , vol.107 , pp. 220-239
    • Munson, P.J.1    Rodbard, D.2
  • 32
    • 0025933034 scopus 로고
    • Identification and characterisation of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensis δ-endotoxins
    • Oddou, P., Hartmann, H. & Geiser, M. (1991). Identification and characterisation of Heliothis virescens midgut membrane proteins binding Bacillus thuringiensis δ-endotoxins. Eur J Biochem 202, 673-680.
    • (1991) Eur J Biochem , vol.202 , pp. 673-680
    • Oddou, P.1    Hartmann, H.2    Geiser, M.3
  • 33
    • 0024961641 scopus 로고
    • Structure of the membrane-pore-forming fragment of colicin A
    • Parker, M. W., Pattus, F., Tucker, A. D. & Tsernoglou, D. (1989). Structure of the membrane-pore-forming fragment of colicin A. Nature 337, 93-96.
    • (1989) Nature , vol.337 , pp. 93-96
    • Parker, M.W.1    Pattus, F.2    Tucker, A.D.3    Tsernoglou, D.4
  • 34
    • 8944237283 scopus 로고
    • Nutrition des insects
    • Poitout, S. & Bues, R. (1972). Nutrition des insects. C R Acad Sci Ser D 274, 3113-3115.
    • (1972) C R Acad Sci Ser D , vol.274 , pp. 3113-3115
    • Poitout, S.1    Bues, R.2
  • 35
    • 0029007022 scopus 로고
    • Single amino acid changes in domain II of Bacillus thuringiensis CryIAb δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles
    • Rajamohan, F., Alcantara, E., Lee, M. K., Chen, X. J., Curtiss, A. & Dean, D. H. (1995). Single amino acid changes in domain II of Bacillus thuringiensis CryIAb δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles. J Bacteriol 177, 2276-2282.
    • (1995) J Bacteriol , vol.177 , pp. 2276-2282
    • Rajamohan, F.1    Alcantara, E.2    Lee, M.K.3    Chen, X.J.4    Curtiss, A.5    Dean, D.H.6
  • 37
    • 0017681196 scopus 로고
    • DNA sequencing with chain-terminating inhibitors
    • Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
    • (1977) Proc Natl Acad Sci USA , vol.74 , pp. 5463-5467
    • Sanger, F.1    Nicklen, S.2    Coulson, A.R.3
  • 38
    • 0025670009 scopus 로고
    • Specificity-determining regions of a lepidopteran-specific insecticidal protein produced by Bacillus thuringiensis
    • Schnepf, H. E., Tomczak, K., Ortega, J. P. & Whiteley, H. R. (1990). Specificity-determining regions of a lepidopteran-specific insecticidal protein produced by Bacillus thuringiensis. J Biol Chem 265, 20923-20930.
    • (1990) J Biol Chem , vol.265 , pp. 20923-20930
    • Schnepf, H.E.1    Tomczak, K.2    Ortega, J.P.3    Whiteley, H.R.4
  • 39
    • 0028297019 scopus 로고
    • Crystal structure of vitelline membrane outer layer protein I (VMO-I): A folding motif with homologous Greek key structures related by an internal three-fold symmetry
    • Shimizu, T., Vassylyev, D. G., Kido. S., Doi, Y. & Morikawa, K. (1994). Crystal structure of vitelline membrane outer layer protein I (VMO-I): a folding motif with homologous Greek key structures related by an internal three-fold symmetry. EMBO J 13, 1003-1010.
    • (1994) EMBO J , vol.13 , pp. 1003-1010
    • Shimizu, T.1    Vassylyev, D.G.2    Kido, S.3    Doi, Y.4    Morikawa, K.5
  • 40
    • 0027997324 scopus 로고
    • Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC δ-endotoxin affects insecticidal specificity
    • Smith, G. P. & Ellar, D. J. (1994). Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC δ-endotoxin affects insecticidal specificity. Biochem J 302, 611-616.
    • (1994) Biochem J , vol.302 , pp. 611-616
    • Smith, G.P.1    Ellar, D.J.2
  • 41
    • 0026585681 scopus 로고
    • Identification and characterisation of a novel Bacillus thuringiensis δ-endotoxin entomocidal to coleopteran and lepidopteran larvae
    • Tailor, R., Tippett, J., Gibb, G., Pells, S., Pike, D., Jordan, L. & Ely, S. (1992). Identification and characterisation of a novel Bacillus thuringiensis δ-endotoxin entomocidal to coleopteran and lepidopteran larvae. Mol Microbiol 6, 1211-1217.
    • (1992) Mol Microbiol , vol.6 , pp. 1211-1217
    • Tailor, R.1    Tippett, J.2    Gibb, G.3    Pells, S.4    Pike, D.5    Jordan, L.6    Ely, S.7
  • 42
    • 0020645619 scopus 로고
    • Bacillus thuringiensis var. israelensis crystal δ-endotoxin: Effects on insect and mammalian cells in vitro and in vivo
    • Thomas, W. E. & Ellar, D. J. (1983). Bacillus thuringiensis var. israelensis crystal δ-endotoxin: effects on insect and mammalian cells in vitro and in vivo. J Cell Sci 60, 181-197.
    • (1983) J Cell Sci , vol.60 , pp. 181-197
    • Thomas, W.E.1    Ellar, D.J.2
  • 43
    • 0027258633 scopus 로고
    • A specific binding protein from Mandata sexta for the insecticidal toxin of Bacillus thuringiensis subsp. berliner
    • Vadlamudi, R. K., Ji, T. H. & Bulla, L. A., Jr (1993). A specific binding protein from Mandata sexta for the insecticidal toxin of Bacillus thuringiensis subsp. berliner. J Biol Chem 286, 12334-12340.
    • (1993) J Biol Chem , vol.286 , pp. 12334-12340
    • Vadlamudi, R.K.1    Ji, T.H.2    Bulla Jr., L.A.3
  • 44
    • 0025228955 scopus 로고
    • Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins
    • Van Rie, J., Jansens, S., Hofte, H., Degheele, D. & Van Mellaert, H. (1990). Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins. Appl Environ Microbiol 56, 1378-1385.
    • (1990) Appl Environ Microbiol , vol.56 , pp. 1378-1385
    • Van Rie, J.1    Jansens, S.2    Hofte, H.3    Degheele, D.4    Van Mellaert, H.5
  • 45
    • 45949125218 scopus 로고
    • Preparation and partial characterisation of amino acid transporting brush border membrane vesicles from the larval midgut of the Cabbage butterfly (Pieris brassicae)
    • Wolfersberger, M., Lüthy, P., Maurer, A., Parenti, P., Sacchi, V. F., Goirdana, B. & Hanozet, G. M. (1987). Preparation and partial characterisation of amino acid transporting brush border membrane vesicles from the larval midgut of the Cabbage butterfly (Pieris brassicae). Comp Biochem Physiol 86A, 301-308.
    • (1987) Comp Biochem Physiol , vol.86 A , pp. 301-308
    • Wolfersberger, M.1    Lüthy, P.2    Maurer, A.3    Parenti, P.4    Sacchi, V.F.5    Goirdana, B.6    Hanozet, G.M.7
  • 46
    • 0026785278 scopus 로고
    • Localised mutagenesis defines regions of the Bacillus thuringiensis δ-endotoxin involved in toxicity and specificity
    • Wu, D. & Aronson, A. I. (1992). Localised mutagenesis defines regions of the Bacillus thuringiensis δ-endotoxin involved in toxicity and specificity. J Biol Chem 267, 2311-2317.
    • (1992) J Biol Chem , vol.267 , pp. 2311-2317
    • Wu, D.1    Aronson, A.I.2


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