Gene expression of acyl coenzyme A: Cholesterol acyltransferase is upregulated in human monocytes during differentiation and foam cell formation

Arteriosclerosis, Thrombosis, and Vascular Biology

Volumn 16, Issue 6, 1996, Pages 809-814

  Wang, Hongxing ^a   Germain, Sandra J ^a   Benfield, Pamela P ^a   Gillies, Peter J ^a  

^a DUPONT COMPANY   (United States)

Author keywords

foam cell; human acyl coenzyme A:cholesterol acyltransferase; macrophage; monocyte; mRNA

Indexed keywords

ACYL COENZYME A; CHOLESTEROL ACYLTRANSFERASE; COMPLEMENTARY DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; OLEOYL COENZYME A;

ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE; FOAM CELL; GENE; GENE EXPRESSION; HUMAN; HUMAN CELL; MACROPHAGE; MONOCYTE; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0029992722     PISSN: 10795642     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.ATV.16.6.809     Document Type: Article

References (21)

1. Stary HC, Chandler AB, Glagov S, Guyton JR, Insull W, Rosenfeld ME, Schaffer SA, Schwartz CJ, Wagner WD, Wissler RW. A definition of intimal, fatty streak, and intermediate lesions of atherosclerosis: a report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association. Arterioscler Thromb. 1994;14:840-856.
2. Gown AM, Tsukada T, Ross R. Human atherosclerosis, II: immunocytochemical analysis of the cellular composition of human atherosclerotic lesions. Am J Pathol 1986;125:191-207.
3. Brown MS, Goldstein JL. Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis. Annu Rev Biochem. 1983;52:223-261.
4. Bell FP. Arterial cholesterol esterification by acylCoA-cholesterol acyltransferase: its possible significance in atherogenesis and its inhibition by drugs. In: Fears R, ed. Pharmacological Control of Hyperlipidaemia. Barcelona, Spain: JR Prous Science Publishers SA; 1986:409-422.
5. Billheimer JT, Gillies PJ. Intracellular cholesterol esterification. In: Esfahani M. Swaney J, eds. Advances in Cholesterol Research. Caldwell, NJ: Telford Press; 1990:7-45.
6. Synouri-Yrettakou S, Mitropoulos KA. Acyl-CoA: cholesterol acyltransferase: transfer of cholesterol to its substrate pool and modulation of activity. Eur J Biochem. 1983;133:299-307.
7. Gillies PJ, Rathgeb KA, Perri MA, Robinson CS. Regulation of acyl-CoA: cholesterol acyltransferase activity in normal and atherosclerotic rabbit aortas: role of a cholesterol substrate pool. Exp Mol Pathol. 1986;44:329-339.
8. Gillies PJ, Robinson CS, Rathgeb KA. Regulation of ACAT activity by a cholesterol substrate pool during the progression and regression phases of atherosclerosis: implications for drug discovery. Atherosclerosis. 1990;83:177-185.
9. Chang CY, Huh HY, Cadigan KM. Chang TY. Molecular cloning and functional expression of human acyl-coenzyme A: cholesterol acyltransferase cDNA in mutant Chinese hamster ovary cells. J Biol Chem. 1993;268:20747-20755.
10. Wang H, Chen X, Fisher EA. N-3 fatty acids stimulate intracellular degradation of apolipoprotein B in rat hepatocytes. J Clin Invest. 1993;91:1380-1389.
11. Boyum A. Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest. 1968;suppl 21:77-89.
12. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem. 1976;72:248-254.
13. Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957;226:497-509.
14. Perrella F. A practical curve-fitting microcomputer program for the analysis of enzyme kinetic data on IBM-PC compatible computers. Anal Biochem. 1988;174:437-447.
15. Geng Y, Kodama T, Hansson GK. Differential expression of scavenger receptor isoforms during monocyte-macrophage differentiation and foam cell formation. Arterioscler Thromb. 1994;14: 798-806.
16. Viallard V, Lacombe C, Nebot C, Caston I, Trocheris V. Acyl-CoA: cholesterol acyltransferase in HT29 cell subpopulations: defect of activity in the undifferentiated cells. Biochim Biophys Acta. 1991; 1085:265-271.
17. Pape ME, Schultz PA, Rea TJ, DeMattos RB, Kieft K, Bisgaier CL, Newton RS, Krause BR. Tissue specific changes in acyl-CoA: cholesterol acyltransferase (ACAT) mRNA levels in rabbits. J Lipid Res. 1995;36:823-838.
18. Mosse PRL, Campbell GR, Wang Z-L, Campbell JH. Smooth muscle phenotypic expression in human carotid arteries, I: comparison of cells from diffuse intimal thickening adjacent to atheromatous plaques with those of the media. Lab Invest. 1985;53:556-562.
19. Watanabe Y, Inaba T, Shimano H, Gotoda T, Yamamoto K, Mokuno H, Sato H, Yazaki Y, Yamada N. Induction of LDL receptor-related protein during the differentiation of monocyte-macrophages: possible involvement in the atherosclerotic process. Arterioscler Thromb. 1994;14:1000-1006.
20. Inaba T, Kawamura M, Gotoda T, Harada K, Shimada M, Ohsuga J, Shimano H, Akanuma Y, Yazaki Y, Yamada N. Effects of platelet-derived growth factor on the synthesis of lipoprotein lipase in human monocyte-derived macrophages. Arterioscler Thromb. 1995;15:522-528.
21. Werb Z, Chin JR. Endotoxin suppresses expression of apoprotein E by mouse macrophages in vivo and in culture: a biochemical and genetic study. J Biol Chem. 1983;258:10640-10648.