Evidence for a role of glucuronosyltransferase in the regulation of androgen action in the human prostatic cancer cell line LNCaP

Journal of Steroid Biochemistry and Molecular Biology

Volumn 57, Issue 3-4, 1996, Pages 225-231

  Guillemette, Chantal ^a   Hum, Dean W ^a   Bélanger, Alain ^a  

^a LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; 3ALPHA HYDROXYSTEROID DEHYDROGENASE; ANDROSTANOLONE; GLUCURONOSYLTRANSFERASE; STEROID 5ALPHA REDUCTASE; TESTOSTERONE 17BETA DEHYDROGENASE;

ANDROGEN METABOLISM; ARTICLE; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; MALE; PROSTATE CANCER;

EID: 0029992517     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-0760(95)00258-8     Document Type: Article

References (26)

1. Berns E. M., de Boer W. and Mulder E.: Androgen-dependent growth regulation of and release of specific protein(s) by the androgen receptor containing human tumor cell line LNCaP. Prostate 9 (1986) 247-259.
2. Sonnenschein C., Olea N., Pasanen M. E. and Soto A. M.: Negative controls of cell proliferation: human prostate cancer cells and androgens. Cancer Res. 49 (1989) 3474-3481.
3. Bélanger C., Veilleux R. and Labrie F.: Stimulatory effects of androgens, estrogens, progestins, and dexamethasone on the growth of the LNCaP human prostate cancer cells. Ann. N.Y. Acad. Sci. 595 (1990) 399-402.
4. Montgomery B. T., Young C. Y. F., Bilhartz P. E. A., Prescott J. L., Thompson F. and Tindall D. J.: Hormonal regulation of prostate-specific antigen (PSA) glycoprotein in the human pro-static adenocarcinoma cell line, LNCaP. Prostate 21 (1992) 63-73.
5. Veldscholte J., Berrevoets C. A., Ris-Stalpers C., Kuiper G. G. J. M., Jenster G., Trapman J., Brinkmann A. O. and Mulder E.: The androgen receptor in LNCaP cells contains a mutation in the ligand binding domain which affects steroid binding characteristic and response to antiandrogens. J. Steroid Biochem. Molec. Biol. 41 (1992) 665-669.
6. Henttu P., Liao S. and Vihko P.: Androgens up-regulate the human prostate-specific antigen messager ribonucleic acid (mRNA), but down-regulate the prostatic acid phosphatase mRNA in the LNCaP cell line. Endocrinology 130 (1992) 766-772.
7. Sugimoto K., Simard J., Haagensen D. E. and Labrie F.: Inverse relationships between cell proliferation and basal or androgen-stimulated apolipoprotein D secretion in LNCaP human prostate cancer cells. J. Steroid Biochem. Molec. Biol. 51 (1994) 167-174.
8. Guillemette C. and Bélanger A.: Glucuronosyltransferase activity in human cancer cell line LNCaP. Molec. Cell. Endocr. 107 (1995) 131-139.
9. Damassa D. A., Lin T. M., Sonnenschein C. and Soto A. M.: Biological effects of sex hormone-binding globulin on androgen-induced proliferation and androgen metabolism in LNCaP prostate cells. Endocrinology 129 (1991) 75-84.
10. Lacoste D., Bélanger A. and Labrie F.: Biosynthesis and degradation of androgens in human prostate cancer cell lines. In Steroid Formation, Degradation, and Action in Peripheral, Normal, and Neoplastic Tissue (Edited by H. Bradlow, L. Castagnetta, S. d'Aquino and F. Labrie.) Ann. N.Y. Acad. Sci. (1990) 389-391.
11. Bélanger A., Brochu M., Lacoste D., Noel C., Labrie F., Dupont A., Cusan L., Caron S. and Couture J.: Steroid glucuronides: human circulatory levels and formation in LNCaP cells. J. Steroid Biochem. Molec. Biol. 40 (1991) 593-598.
12. Simard J., de Launoit ?., Haagensen D. E. and Labrei F.: Additive stimulatory effect of glucocorticoids and androgens on basal and estrogen-repressed apolipoprotein-D messenger ribonucleic acid levels and secretion in human breast cancer cells. Endocrinology 130 (1992) 1115-1121.
13. Bélanger A., Rong P. M., Sha L. and Labrie F.: Characterization and measurement of prostatic-specific antigen using monoclonal antibodies. Clin. Invest. Med. 16 (1993) 409-415.
14. Rodbard D. and Lewald J. E.: Computer analysis of radioligand assay and radioimmunoassay data. In Second Karolinska Symposium on Research Methods in Reproductive Endocrinology. Acta Endocr. (Copenhagen) 64 (1970) 79-103.
15. Rodbard D.: Apparent positive cooperative effect in cyclic AMP and corticosteroid production by isolated adrenal cells in response to ACTH analogs. Endocrinology 94 (1974) 1427-1437.
16. Kramer C. Y.: Extension of multiple range tests to group means with unique number of replications. Biometrics 12 (1956) 307-310.
17. Chen F., Ritter J. K., Wang M. G., McBridge W., Lubet R. A. and Owens I. S.: Characterization of a cloned human dihydrotestosterone/androstadiol UDP-glucuronosyltransferase and its comparison to other steroid isoforms. Biochemistry 32 (1993) 10,648-10,657.
18. Green M., Eyitayo M. O. and Tephly R. T.: Stable expression of a human liver UDP-glucuronosyltransferase (UGT2B15) with activity toward steroid and xenobiotic substrates. Drug Metab. Dispos. 22 (1994) 799-805.
19. Guillemette C., Hum D. W. and Bélanger A.: Specificity of glucuronosyltransferase activity in the human cancer cell line LNCaP, evidence for the presence of at least two glucuronosyl-transferase enzymes. J. Steroid Biochem. Molec. Biol. 55 (1995) in press.
20. Bélanger G., Beaulieu M., Lévesque E., Marcotte B., Guillemette C., Hum D. W. and Bélanger A.: Expression of transcripts encoding steroid UDP-glucuronosyltransferases in human prostate hyperplastic tissue and the LNCaP cell line. Molec. Cell Endocr. 113 (1995) 165-173.
21. Miners J. O. and Mackenzie P. I.: Glucuronidation in humans. Pharmac. Ther. 51 (1991) 347-369.
22. Burchell B. and Coughtrie M. W. H.: UDP-glucuronosyltransferase. Pharmac. Ther. 43 (1989) 261-289.
23. Burchell B., Nebert D. W., Nelson D. R., Bock K. W., Iyanagi T., Jansen P. L. M., Lancet D., Mulder G. J., Chowdury J. R., Siest G., Tephly T. R. and Mackenzie P. I.: The UDP glucuronosyltransferase gene superfamily: suggested nomenclature based on evolutionary divergence. DNA Cell Biol. 10 (1991) 487-494.
24. Mackenzie P. I., Rodbourne L. and Stanks S.: Steroid UDP glucuronosyltransferase. J. Steroid Biochem. Molec. Biol. 43 (1992) 1099-1105.
25. Bélanger A., Couture J., Caron S. and Roy R.: Determination of non-conjugated and conjugated steroid levels in plasma and prostate after separation on C-18 columns. Ann. N.Y. Acad. Sci. 595 (1990) 251-259.
26. Bélanger A., Candas B., Dupont A., Cusan L., Diamond P., Gomez J. L. and Labrie F.: Changes in serum concentrations of conjugated and unconjugated steroids in 40-to 80-year-old men. J. Clin. Endocr. Metab. 79 (1995) 1086-1090.