A light-scattering assay for lymphocyte shape and its application to T and B lymphocyte responses to cultured high-walled endothelial cells

Journal of Immunological Methods

Volumn 192, Issue 1-2, 1996, Pages 179-185

  Harris, Harriet ^a   Jolley, Caroline ^a   Miller, Nigel G ^a  

^a BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

flow cytometry; high walled endothelium; lymphocyte

Indexed keywords

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL SHAPE; ENDOTHELIUM CELL; FLOW CYTOMETRY; LYMPH NODE CELL; NONHUMAN; PRIORITY JOURNAL; RAT; T LYMPHOCYTE;

ANIMALIA;

EID: 0029992386     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00050-6     Document Type: Article

References (21)

1. Ager, A. (1987) Isolation and culture of high endothelial cells from rat lymph nodes. J. Cell Sci. 87, 133.
2. Ager, A. and Mistry, S. (1988) Interaction between lymphocytes and cultured high endothelial cells: an in vitro model of lymphocyte migration across high endothelial venule endothelium. Eur. J. Immunol. 18, 1265.
3. Ager, A. and Wood, A. (1994) Downregulation of L-selectin following binding to high endothelium: a prerequisite for transendothelial migration? Lymphology 27 (suppl.), 187.
4. Butcher, E.G. (1991) Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67, 1033.
5. Fukumoto, T., McMaster, W.R. and Williams, A.F. (1982) Mouse monoclonal antibodies against rat major histocompatability antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur. J. Immunol. 12, 237.
6. Harris, H. (1991) The stimulation of lymphocyte motility and its inhibition by pertussis toxin. Int. Immunol. 3, 535.
7. Harris, H. and Miyasaka, M. (1995) Reversible stimulation of lymphocyte motility by cultured high endothelial cells: mediation by L-selectin. Immunology 85, 47.
8. Haston, W.S. and Shields, J.M. (1984) Contraction waves in lymphocyte locomotion. J. Cell Sci. 68, 227.
9. 1 integrin (VLA-4) affinity. J. Cell Sci. 104, 1049.
10. Hünig, T., Wallny, H.-J., Hartley, J.K., Lawetzky, A. and Tiefenthaler, G. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J. Exp. Med. 169, 73.
11. Komai-Koma, M., Liew, F.Y. and Wilkinson, P.C. (1995) Interactions between Il-4. anti-CD40, and anti-immunoglobulin as activators of locomotion of human B cells. J. Immunol. 155, 1110.
12. Matthews, N., Franklin, R.J. and Kendrick, D.A. (1995) Structure-activity relationships of phenothiazines in inhibiting lymphocyte motility as determined by a novel flow cytometric assay. Biochem. Pharmacol. 50, 1053.
13. May, M.J., Entwistle, G., Humphries, M.J. and Ager, A. (1993) VCAM-1 is a CS1 peptide-inhibitable adhesion molecule expressed by lymph node high endothelium. J. Cell Sci. 106, 109.
14. McMaster, W.R. and Williams, A.F. (1979) Identification of Ia glycoproteins in rat thymus and purified from rat spleen. Eur. J. Immunol. 9, 426.
15. Robinson, A.P., Puklavec, M. and Mason, D.W. (1986) MRC OX-52: a rat T cell antigen. Immunology 57, 527.
16. Springer, T.A. (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301.
17. 1. J Cell Sci. 101, 885.
18. Wanger, L., Otteskog, P. and Sundqvist, K.-G. (1985) Anchorage and lymphocyte function. Acquisition of spontaneous motile behaviour by human blood lymphocytes and its modulation by concanavalin A. Immunology 56, 425.
19. Wilkinson, P.C. (1986) The locomotor capacity of human lymphocytes and its enhancement by cell growth. Immunology 57, 281.
20. Wilkinson, P.C. and Islam, L.N. (1989) Recombinant Il-4 and IFN-γ activate locomotor capacity in human B lymphocytes. Immunology 67, 237.
21. Wilkinson, P.C., Lackie, J.M., Haston, W.S. and Islam, L.N. (1988) Effects of phorbol esters on shape and locomotion of human blood lymphocytes. J. Cell Sci. 90, 645.