Evidence that the nuclease activities associated with the herpes simplex type 1 DNA polymerase are due to the 3′-5′ exonuclease

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4816-4818

  Hall, Jennifer D ^a   Orth, Kimberly L ^a   Claus Walker, Debra ^a  

^a University of Arizona   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; NUCLEASE; PHOSPHODIESTERASE I;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; HERPES SIMPLEX VIRUS 1; NONHUMAN; PRIORITY JOURNAL; VIRUS REPLICATION;

ANIMALS; CELL LINE; DNA-DIRECTED DNA POLYMERASE; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; HERPESVIRUS 1, HUMAN; HUMANS; MUTATION; SPODOPTERA; SUBSTRATE SPECIFICITY; VIRAL PROTEINS;

EID: 0029992358     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4816-4818.1996     Document Type: Article

References (11)

1. Blanco, L., A. Bernad, M. A. Blasco, and M. Salas. 1991. A general structure for DNA-dependent DNA polymerases. Gene 100:27-38.
2. Crute, J. J., and I. R. Lehman. 1989. Herpes simplex-1 DNA polymerase. Identification of an intrinsic 5′-3′ exonuclease with ribonuclease H activity. J. Biol. Chem. 264:19266-19270.
3. Gotllieb, J., A. I. Marcy, D. M. Coen, and M. D. Challberg. 1990. The herpes simplex virus type 1 UL42 gene product: a subunit of DNA polymerase that functions to increase processivity. J. Virol. 64:5976-5987.
4. Hall, J. D., K. L. Orth, K. L. Sander, B. M. Swihart, and R. A. Senese. 1995. Mutations within conserved motifs in the 3′-5′ exonuclease domain of herpes simplex virus DNA polymerase. J. Gen. Virol. 76:2999-3008.
5. Knopf, C. W., and R. Strick. 1994. Herpes simplex virus type 1 DNA polymerase: Eukaryotic model enzyme and principal target of antiviral therapy, p. 87-135. In Y. Becker and G. Darai (ed.), Frontiers of virology, vol. 3. Springer-Verlag, Berlin.
6. Knopf, C. W., and K. Weisshart. 1990. Comparison of exonucleolytic activities of herpes simplex virus type-1 DNA polymerase and DNase. Eur. J. Biochem. 191:263-273.
7. Knopf, K.-W. 1979. Properties of herpes simplex virus DNA polymerase and characterization of its associated exonuclease activity. Eur. J. Biochem. 98: 231-244.
8. Marcy, A. I., P. D. Olivo, M. D. Challberg, and D. M. Coen. 1990. Enzymatic activities of overexpressed herpes simplex virus DNA polymerase purified from recombinant baculovirus-infected insect cells. Nucleic Acids Res. 18: 1207-1215.
9. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
10. Weisshart, K., and C. W. Knopf. 1988. The herpes simplex virus type 1 DNA polymerase. Polypeptide structure and antigenic domains. Eur. J. Biochem. 174:707-716.
11. Weisshart, K., A. A. Kuo, C. B. C. Hwang, K. Kumura, and D. M. Coen. 1994. Structural and functional organization of herpes simplex virus DNA polymerase investigated by limited proteolysis. J. Biol. Chem. 269:22788-22796.