Comparative study of three PCR assays with antigenaemia and serology for the diagnosis of HCMV infection in thoracic transplant recipients

Journal of Medical Virology

Volumn 49, Issue 2, 1996, Pages 137-144

  Barber, Lisa ^a   Egan, Jim J ^b   Lomax, Joyce ^a   Yonan, Nizar ^b   Deiraniya, Abdul K ^b   Turner, Andrew J ^a   Woodcock, Ashley A ^b   Fox, Andrew J ^a  

^a WITHINGTON HOSPITAL   (United Kingdom)

^b WYTHENSHAWE HOSPITAL   (United Kingdom)

Author keywords

DNA; Glycoprotein B; HCMV; Major immediate early region,; PCR; Pp150; Structural phosphoprotein

Indexed keywords

GANCICLOVIR; GLYCOPROTEIN B; PHOSPHOPROTEIN; UNCLASSIFIED DRUG; VIRUS GLYCOPROTEIN;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HEART TRANSPLANTATION; HUMAN; LUNG TRANSPLANTATION; POLYMERASE CHAIN REACTION; SERODIAGNOSIS; VIRUS DETECTION;

ANTIGENS, VIRAL; ANTIVIRAL AGENTS; BASE SEQUENCE; CYTOMEGALOVIRUS INFECTIONS; DRUG MONITORING; HEART-LUNG TRANSPLANTATION; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION;

CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5;

EID: 0029991685     PISSN: 01466615     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9071(199606)49:2<137::AID-JMV11>3.0.CO;2-B     Document Type: Article

References (31)

1. Bauer, HM, Ting, Y, Greer, CE, Chambers, JC, Tashiro, CJ, Chimera, J, Reingold, A, Manos, MM (1991): Genital human papillomavirus infection in female university students as determined by a PCR-based method. Journal of the American Medical Association 265:472-477.
2. Bitsch, A, Kirchner, H, Dennin, R, Hoyer, J, Frics, L, Steinhoff, J, Sack, K, Bein, G (1993): The long persistence of CMV DNA in the blood of renal transplant patients after recovery from CMV infection. Transplantation 56:108-113.
3. Brytting, M, Wahlberg, J, Lundeberg, J, Wahren, B, Uhlen, M, Sundqvist, V (1992a): Variations found in the cytomegalovirus major immediate early gene by direct genomic sequencing. Journal of Clinical Microbiology 30:955-960.
4. Brytting, M, Zu, W, Wahren, B, Sundqvist, V (1992b): Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections. Journal of Clinical Microbiology 30:1937-1941.
5. Chou S (1992a): Comparative analysis of sequence variation in gp 116 and gp55 components of glycoprotein B of human cytomegalovirus. Virology 188:388-390.
6. Chou, S (1992b): Effect of interstrain variation on diagnostic DNA amplification of the cytomegalovirus major immediate-early gene region. Journal of Clinical Microbiology 30:2307-2310.
7. Chou, S, Dennison, KM (1991): Analysis of interstrain variation in cytomegalovirus glycoprotein B sequences encoding neutralization-related epitopes. Journal of Infectious Diseases 163:1229-1234.
8. Darlington, J, Super, M, Patel, K, Grundy, JE, Griffiths, PD, Emery, VC (1991): Use of the polymerase chain reaction to analyse sequence variation within a major neutralising epitope of glycoprotein B (gp58) in clinical isolates of human cytomegalovirus. Journal of General Virology 72:1985-1989.
9. Demmler, GJ, Buffone, GJ, Schimbor, CM, May, RA (1988): Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification. Journal of Infectious Diseases 158:1177-1184.
10. Egan, JJ, Barber, L, Lomax, J, Fox, A, Yonan, N, Rahman, AN, Campbell, CS, Deiraniya, AK, Carroll, KB, Craske, J, Turner, A, Woodcock, AA (1995): Detection of human cytomegalovirus antigenaemia: A rapid diagnostic technique for predicting cytomegaloviral infection/pneumonitis in lung and heart transplant recipients. Thorax 50:9-13.
11. Einsele, H, Ehninger, G, Steidle, M, Vallbracht, A, Müller, M, Schmidt, H, Saal, JG, Waller, HD, Müller, CA (1991): Polymerase chain reaction to evaluate antiviral therapy for cytomegalovirus disease. Lancet 338:1170-1172.
12. Ettinger, NA, Bailey, TC, Trulock, EP, Storch, GA, Anderson, D, Raab, S, Spitznagel, EL, Dresler, C, Cooper, JD (1993): Cytomegalovirus infection and pneumonitis: Impact after isolated lung transplantation. American Review of Respiratory Disease 147:1017-1023.
13. Fox, JC, Griffiths, PD, Emery, VC (1992): Quantification of human cytomegalovirus DNA using the polymerase chain reaction. Journal of General Virology 73:2405-2408.
14. Fox, JC, Kidd, IM, Griffiths, PD, Sweny, P, Emery, VC (1995): Longitudinal analysis of cytomegalovirus in renal transplant recipients using a quantitative polymerase chain reaction: correlation with disease. Journal of General Virology 76:309-319.
15. Gerna, G, Zipeto, D, Parea, M, Revello, MG, Silini, E, Percivalle, E, Zavattoni, M, Grossi, P, Milanesi, G (1991): Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenaemia and DNAemia. Journal of Infectious Diseases 164:488-498.
16. Jiwa, NM, van Gemert, GW, Raap, AK (1989): Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 48:72-77.
17. Koskinen, PK, Nieminen, MS, Mattila, SP, Hayry, PJ, Lautenschlager, IT (1993): The correlation between symptomatic CMV infection andCMV antigenaemia in heart allograft recipients. Transplantation 55:547-551.
18. Landry, ML, Ferguson, D (1993): Comparison of quantitative cytomegalovirus antigenaemia assay with culture methods and correlation with clinical disease. Journal of Clinical Microbiology 31: 2851-2856.
19. Morris, DJ, Fox, AJ, Grint, PCA, Bell, EJ, Lomax, J, McCartney, RA (1990): Cytomegalovirus, varicella zoster and coxsackie B virus specific IgM antibody response in children with cancer. Serodiagnosis and Immunotherapy in Infectious Disease 4:115-120.
20. Morris, DJ, Lomax, J, Craske, J, Longson, M, Fox, AJ (1987): Effects of centrifugal enhancement of infectivity on the rapid detection of human cytomegalovirus in cell culture by immunofluorescence using a monoclonal antibody to immediate early nuclear antigen. Serodiagnosis and Immunotherapy in Infectious Disease 1:141-152.
21. Sambrook, J, Fritsch, EF, Maniatis, T (eds), "Molecular Cloning: A Laboratory Manual," Second Ed. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
22. Schäfer, P, Braun, RW, Möhring, K, Henco, K, Rang, J, Wendland, T, Kühn, JE (1993): Quantitative determination of human cytomegalovirus target sequences in peripheral blood leukocytes by nested polymerase chain reaction and temperature gradient gel electrophoresis. Journal of General Virology 74:2699-2707.
23. Smyth, RS, Scott, JP, Borysiewicz, LK, Sharples, LD, Stewart, S, Wreghitt, TG, Gray, JJ, Higenbottam, TW, Wallwork J (1991): Cytomegalovirus infection in heart-lung transplant recipients: Risk factors, clinical associations and response to treatment. Journal of Infectious Diseases 164:1045-1050.
24. Storch, GA, Buller, RS, Bailey, TC, Ettinger, NA, Langlois, T, Gaudteault-Keener, M, Welby, PL (1994): Comparison of PCR and pp65 antigenaemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients. Journal of Clinical Microbiology 32:997-1003.
25. Taylor-Weideman, J, Hayhurst, GP, Sissons, JGP, Sinclair, JH (1993): Polymorphonuclear cells are not sites of persistence of human cytomegalovirus in healthy individuals. Journal of General Virology 74:265-268.
26. van den Berg, AP, Klimpmaker, IJ, Haagsma, EB, Scholten-Sampson, A, Bijleveld, CMA, Schirm, J, van der Giessen, M, Sloff, MJH, The, TH (1991): Antigenaemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. Journal of Infectious Diseases 164:265-270.
27. van der Bij, W, van Son, WJ, van der Berg, APM, Tegzess, AM, Torensma, R, The, TH (1989): Cytomegalovirus (CMV) Antigenaemia: Rapid diagnosis and relationship with CMV-associated clinical syndromes in renal allograft recipients. Transplant Proceedings 21:2061-2064.
28. Vecchio, TJ (1966): Predictive value of a single diagnostic test in unselected populations. New England Journal of Medicine 274: 1171-1173.
29. Wolf, DG, Spector SA (1993): Early diagnosis of human cytomegalovirus disease in transplant recipients by DNA amplification in plasma. Transplantation 56:330-334.
30. Wreghitt, TG, Hakim, M, Gray, JJ, Kucia, S, Wallwork, J, English, TAH (1988): Cytomegalovirus infections in heart and heart and lung transplant recipients. Journal of Clinical Pathology 41:660-667.
31. Zipeto, D, Silini, E, Parea, M, Percivalle, E, Zavattoni, M, Di Matteo A, Milanesi, G, Gerna, G (1990): Identification of human cytomegalovirus isolates by the polymerase chain reaction. Microbiologica 13:297-304.