메뉴 건너뛰기




Volumn 288, Issue 4, 1996, Pages 203-210

Multiparameter flow cytometric characterization of epidermal cell suspensions prepared from normal and hyperproliferative human skin using an optimized thermolysin-trypsin protocol

Author keywords

Flow cytometry; Human epidermis; Separation method; Tape stripping; Thermolysin trypsin

Indexed keywords

KERATIN; THERMOLYSIN; TRYPSIN; VIMENTIN;

EID: 0029990997     PISSN: 03403696     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02505225     Document Type: Article
Times cited : (40)

References (30)
  • 2
    • 0027248633 scopus 로고
    • Flow cytometric identification of proliferative subpopulations within normal human epidermis and the localization of the primary hyperproliferative population in psoriasis
    • Bata-Csorgo Z, Hammerberg C, Voorhees JJ, Cooper KD (1993) Flow cytometric identification of proliferative subpopulations within normal human epidermis and the localization of the primary hyperproliferative population in psoriasis. J Exp Med 178:1271-1281
    • (1993) J Exp Med , vol.178 , pp. 1271-1281
    • Bata-Csorgo, Z.1    Hammerberg, C.2    Voorhees, J.J.3    Cooper, K.D.4
  • 3
    • 0842348208 scopus 로고
    • Cell kinetics
    • Mier PD, Kerkhof PCM van de (eds) Churchill Livingstone, Edinburgh
    • Bauer FW (1986) Cell kinetics. In: Mier PD, Kerkhof PCM van de (eds) Textbook of psoriasis. Churchill Livingstone, Edinburgh, pp 100-112
    • (1986) Textbook of Psoriasis , pp. 100-112
    • Bauer, F.W.1
  • 4
    • 0001944768 scopus 로고
    • Flow cytometric methods in human skin with respect to cell cycle kinetics
    • Wright NB, Camplejohn RS (eds) Churchill Livingstone, Edinburgh
    • Bauer FW, Boezeman JBM (1983) Flow cytometric methods in human skin with respect to cell cycle kinetics. In: Wright NB, Camplejohn RS (eds) Psoriasis: cell proliferation. Churchill Livingstone, Edinburgh, pp 104-116
    • (1983) Psoriasis: Cell Proliferation , pp. 104-116
    • Bauer, F.W.1    Boezeman, J.B.M.2
  • 5
    • 0018906024 scopus 로고
    • Flow cytometry as a tool for the study of cell kinetics in epidermis
    • Bauer FW, Crombag NH, Grood RM de, Jongh GJ de (1980) Flow cytometry as a tool for the study of cell kinetics in epidermis. Br J Dermatol 102:629-639
    • (1980) Br J Dermatol , vol.102 , pp. 629-639
    • Bauer, F.W.1    Crombag, N.H.2    De Grood, R.M.3    De Jongh, G.J.4
  • 8
    • 0023787407 scopus 로고
    • Enzymatic quantification of polymorphonuclear leucocytes in normal and psoriatic skin following standardized injury
    • Chang A, Jongh GJ de, Mier PD, Kerkhof PCM van de (1988) Enzymatic quantification of polymorphonuclear leucocytes in normal and psoriatic skin following standardized injury. Clin Exp Dermatol 13:62-66
    • (1988) Clin Exp Dermatol , vol.13 , pp. 62-66
    • Chang, A.1    De Jongh, G.J.2    Mier, P.D.3    Van De Kerkhof, P.C.M.4
  • 10
    • 0027208118 scopus 로고
    • Improvement of human keratinocyte isolation and culture using thermolysin
    • Germain L, Rouabhia M, Guignard R, Bouvard V, Auger A (1993) Improvement of human keratinocyte isolation and culture using thermolysin. Burns 19:99-104
    • (1993) Burns , vol.19 , pp. 99-104
    • Germain, L.1    Rouabhia, M.2    Guignard, R.3    Bouvard, V.4    Auger, A.5
  • 13
    • 0018616522 scopus 로고
    • Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization
    • Gommans JM, Bergers M, Erp PEJ van, Hurk JJ van den, Mier PD, Roelfzema H (1979) Studies on the plasma membrane of normal and psoriatic keratinocytes. I. Preparation of material and morphological characterization. Br J Dermatol 101:407-412
    • (1979) Br J Dermatol , vol.101 , pp. 407-412
    • Gommans, J.M.1    Bergers, M.2    Van Erp, P.E.J.3    Van Den Hurk, J.J.4    Mier, P.D.5    Roelfzema, H.6
  • 14
    • 0017276508 scopus 로고
    • Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization
    • Stockh
    • Hentzer B, Kobayashi T (1976) Dissociation of human adult epidermal cells by disulfide-reducing agents and subsequent trypsinization. Acta Derm Venereol (Stockh) 56:19-25
    • (1976) Acta Derm Venereol , vol.56 , pp. 19-25
    • Hentzer, B.1    Kobayashi, T.2
  • 15
    • 0028077241 scopus 로고
    • TOPRO-3 iodide, a novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry
    • Hooijdonk CAEM van, Glade CP, Erp PEJ van (1994) TOPRO-3 iodide, a novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry. Cytometry 17:185-189
    • (1994) Cytometry , vol.17 , pp. 185-189
    • Van Hooijdonk, C.A.E.M.1    Glade, C.P.2    Van Erp, P.E.J.3
  • 16
  • 17
    • 0020574587 scopus 로고
    • Separation of the epidermal sheet by dispase
    • Kitano Y, Okada N (1983) Separation of the epidermal sheet by dispase. Br J Dermatol 108:555-560
    • (1983) Br J Dermatol , vol.108 , pp. 555-560
    • Kitano, Y.1    Okada, N.2
  • 18
    • 0025955484 scopus 로고
    • Isolation, detection, and amplification of intact mRNA from dermatotome strips, epidermal sheets, and sorted epidermal cells
    • Longley J, Ding TG, Cuono C, Durden F, Crooks C, Hufeisen S, Eckert R, Wood GS (1991) Isolation, detection, and amplification of intact mRNA from dermatotome strips, epidermal sheets, and sorted epidermal cells. J Invest Dermatol 97:974-979
    • (1991) J Invest Dermatol , vol.97 , pp. 974-979
    • Longley, J.1    Ding, T.G.2    Cuono, C.3    Durden, F.4    Crooks, C.5    Hufeisen, S.6    Eckert, R.7    Wood, G.S.8
  • 19
    • 0025215151 scopus 로고
    • Flow cytometric quantification of human epidermal cells expressing keratin 16 in vivo after standardized trauma
    • Mare S de, Erp PEJ van, Ramaekers FCS, Kerkhof PCM van de (1990) Flow cytometric quantification of human epidermal cells expressing keratin 16 in vivo after standardized trauma. Arch Dermatol 282:126-130
    • (1990) Arch Dermatol , vol.282 , pp. 126-130
    • De Mare, S.1    Van Erp, P.E.J.2    Ramaekers, F.C.S.3    Van De Kerkhof, P.C.M.4
  • 20
    • 0023221184 scopus 로고
    • The role of immunochemistry in diagnostic pathology
    • Mason D, Gatter K (1987) The role of immunochemistry in diagnostic pathology. J Clin Pathol 40:1042-1054
    • (1987) J Clin Pathol , vol.40 , pp. 1042-1054
    • Mason, D.1    Gatter, K.2
  • 21
    • 0000295762 scopus 로고
    • Examination of the epidermis by the strip method of removing horny layers. II. Observations on thickness of the horny layer, and on mitotic activity after stripping
    • Pinkus H (1951) Examination of the epidermis by the strip method of removing horny layers. II. Observations on thickness of the horny layer, and on mitotic activity after stripping. J Invest Dermatol 16:383-386
    • (1951) J Invest Dermatol , vol.16 , pp. 383-386
    • Pinkus, H.1
  • 23
    • 0025859919 scopus 로고
    • Inhibition of basal cell proliferation during storage of detached cultured epidermal keratinocyte sheets
    • Stockh
    • Poumay Y, Boucher F, Degen A, Leloup R (1991) Inhibition of basal cell proliferation during storage of detached cultured epidermal keratinocyte sheets. Acta Derm Venereol (Stockh) 71: 195-198
    • (1991) Acta Derm Venereol , vol.71 , pp. 195-198
    • Poumay, Y.1    Boucher, F.2    Degen, A.3    Leloup, R.4
  • 24
    • 0027977117 scopus 로고
    • Basal detachment of the epidermis using dispase: Tissue spatial organization and fate of integrin a6b4 and hemidesmosomes
    • Poumay Y, Roland IH, Leclercq-Smekens M, Leloup R (1994) Basal detachment of the epidermis using dispase: tissue spatial organization and fate of integrin a6b4 and hemidesmosomes. J Invest Dermatol 102:111-117
    • (1994) J Invest Dermatol , vol.102 , pp. 111-117
    • Poumay, Y.1    Roland, I.H.2    Leclercq-Smekens, M.3    Leloup, R.4
  • 25
    • 0004310788 scopus 로고
    • The influence of proteases on the colony-forming efficiency of human keratinocytes in culture
    • Raxworthy MJ, Cunliffe WJ, Wood EJ (1987) The influence of proteases on the colony-forming efficiency of human keratinocytes in culture. Biochem Soc Trans 15:519-520
    • (1987) Biochem Soc Trans , vol.15 , pp. 519-520
    • Raxworthy, M.J.1    Cunliffe, W.J.2    Wood, E.J.3
  • 26
    • 0022550538 scopus 로고
    • Onset of epidermal differentiation in rapidly proliferating basal keratinocytes
    • Régnier M, Vaigot P, Darmon M. Pruniéras M (1986) Onset of epidermal differentiation in rapidly proliferating basal keratinocytes. J Invest Dermatol 87:472-476
    • (1986) J Invest Dermatol , vol.87 , pp. 472-476
    • Régnier, M.1    Vaigot, P.2    Darmon, M.3    Pruniéras, M.4
  • 27
    • 0024326408 scopus 로고
    • Two binding sites for Ki67 related to quiescent and cycling cells in human epidermis
    • Stockh
    • Rijzewijk JJ, Erp PEJ van, Bauer FW (1989) Two binding sites for Ki67 related to quiescent and cycling cells in human epidermis. Acta Derm Venereol (Stockh) 69:512-515
    • (1989) Acta Derm Venereol , vol.69 , pp. 512-515
    • Rijzewijk, J.J.1    Van Erp, P.E.J.2    Bauer, F.W.3
  • 29
    • 0024500998 scopus 로고
    • Thermolysin treatment: A new method for dermo-epidermal separation
    • Walzer C, Benathan M, Frenk E (1989) Thermolysin treatment: a new method for dermo-epidermal separation. J Invest Dermatol 92:78-81
    • (1989) J Invest Dermatol , vol.92 , pp. 78-81
    • Walzer, C.1    Benathan, M.2    Frenk, E.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.