Effects of injecting calcium-buffer solutions on [Ca2+]i in voltage-clamped snail neurons

Biophysical Journal

Volumn 70, Issue 5, 1996, Pages 2120-2130

  Kennedy, Helen J ^a   Thomas, Roger C ^a,b  

^a Dept Anat Sch Med Sci U   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM ION; FURA 2;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; DEPOLARIZATION; DISSOCIATION CONSTANT; ELECTRIC POTENTIAL; MICROELECTRODE; NERVE CELL; NONHUMAN; SNAIL; VOLTAGE CLAMP;

ANIMALS; BUFFERS; CAFFEINE; CALCIUM; CHELATING AGENTS; EGTAZIC ACID; ELECTROPHYSIOLOGY; FURA-2; GANGLIA, INVERTEBRATE; HELIX (SNAILS); KINETICS; MEMBRANE POTENTIALS; MICROELECTRODES; MICROINJECTIONS; NEURONS; PATCH-CLAMP TECHNIQUES; SPECTROMETRY, FLUORESCENCE; TIME FACTORS;

ANIMALIA; GASTROPODA;

EID: 0029990452     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(96)79778-3     Document Type: Article

References (38)

1. Alvarez-Leefmans, F. J., T. J. Rink, and R. Y. Tsien. 1981. Free calcium ions in neurons of Helix aspersa measured with ion-selective microelectrodes. J. Physiol. 315:531-548.
2. 2+ microelectrode with subnanomolar detection limit. Pflügers Archiv. Eur. J. Physiol. 409:223-228.
3. Baker, P. F., A. L. Hodgkin, and E. B. Ridgeway. 1971. Depolarization and calcium entry in squid giant axons. J. Physiol. 218:709-755.
4. 1 in intact rabbit cardiac myocytes. Biophys. J. 68:1453-1460.
5. Baylor, S. M., A. B. Harkins, and N. Kurebayashi. 1994. Response to Westerblad and Allen. Biophys. J. 66:927-928.
6. 2+ in muscle with Ca electrodes and aequorin. J. Gen. Physiol. 98:1141-1160.
7. Bleakman, D., J. D. Roback, B. H. Wainer, R. J. Miller, and N. L. Harrison. 1993. Calcium homeostasis in rat septal neurons in tissue culture. Pflügers Arch. Eur. J. Physiol. 600:257-267.
8. 2+],. J. Physiol. 450:217-246.
9. Gorman, A. L. F., S. Levy, E. Nasi, and D. Tillotson. 1984. Intracellular calcium measured with calcium-sensitive micro-electrodes and Arsenazo in in voltage-clamped Aplysia neurons. J Physiol. 353:127-142.
10. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260:3440-3450.
11. Harrison, S. M., and D. M. Bers. 1989. Correction of proton and Ca association constants of EGTA for temperature and ionic strength. Am. J. Physiol. 256:C1250-C1256.
12. Hollingworth, S., A. B. Harkins, N. Kurebayashi, M. Konishi, and S. M. Baylor. 1992. Excitation-contraction coupling in intact frog skeletal muscle fibres injected with mmolar concentrations of fura-2. Biophys. J. 63:224-234.
13. D for Ca. Cell Calcium. 12.415-422.
14. Kennedy, H. J., and R. C. Thomas. 1994. The effects of injecting calcium buffer solutions on intracellular calcium in snail neurons in isolated ganglia. J. Physiol. 479:11P.
15. Kennedy, H. J., and R. C Thomas. 1995. Intracellular calcium and its sodium-independent regulation in voltage-clamped snail neurons. J. Physiol. 484:533-548.
16. Konishi, M., A. Olson, S. Hollingworth, and S. M. Baylor. 1988. Myopiasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements. Biophys. J. 54:1089-1104.
17. Kostyuk, P. G., S. L. Mironov, A. V. Tepikin, and P. V. Belan. 1989. Cytoplasmic free Ca in isolated snail neurons as revealed by the fluorescent probe fura-2: mechanisms of Ca recovery after Ca load and Ca release from intracellular sites. J. Membr. Biol. 110:11-18.
18. 2+ in voltage-clamped Aplysia neurons. Brain Res. 474:332-342.
19. McGuigan, J. A. S., D. Luthi, and A. Buri. 1991. Calcium buffer solutions and how to make and use them: a do it yourself guide. Can. J. Physiol. Pharmacol. 69:1733-1749.
20. Meech, R. W., and N. B. Standen. 1975. Potassium activation in Helix aspersa neurons under voltage-clamp: a component mediated by calcium influx. J. Physiol. 249.211-239.
21. Muller, T. H., L. D. Partridge, and D. Swandulla. 1993. Calcium buffering in bursting Helix pacemaker neurons. Pflügers Arch. Eur. J. Physiol. 425:499-505.
22. Neher, E., and G. J. Augustine. 1992. Calcium gradients and buffers in bovine chromaffin cells. J. Physiol. 450:273-301.
23. 2+ in caffeine-treated bullfrog sympathetic neurons. J. Physiol. 350:513-528.
24. i in voltage-clamped snail Helix aspersa neurons. J. Physiol. 489:19-28.
25. Ou-Yaag, Y., T. Kristian, P. Mellergard, and B. K. Siesjo. 1994. The influence of pH on glutamate and depolarisation-induced increases of intracellular calcium concentration in cortical neuron in primary culture. Brain Res. 646:65-72.
26. Poeme, M. 1990. Alteration of intracellular Fura-2 fluorescence by viscosity: a simple correction. Cell Calcium. 11:85-91.
27. Ross, W. N. 1993. Calcium on the level. Biophys. J. 64:1655-1656.
28. Schwiening, C. J., H. J. Kennedy, and R. C. Thomas. 1993. Calcium-hydrogen exchange by the plasma membrane Ca-ATPase of voltage-clamped snail neurons. Proc. R. Soc. Lond. 253:285-289.
29. 2+] in hypoxia and ischemia in rat brain in vivo. J. Gen. Physiol. 95:837-866.
30. Stuenkel, E. L. 1994. regulation of intracellular calcium and calcium buffering properties of rat isolated neurohypophysial nerve endings. J. Physiol 481:251-271.
31. Tatsumi, H., and Y. Katayama. 1993. Regulation of the intracellular free calcium concentration in acutely dissociated neurons from rat nucleus basalis. J. Physiol. 464:165-181.
32. Taylor, P. S., and R. C. Thomas. 1984. The effect of leakage on microelectrode measurements of intracellular sodium activity in crab muscle fibres. J. Physiol. 352:539-550.
33. Thayer, S. A., and R. J. Miller. 1990. Regulation of the intracellular free calcium concentration in single rat dorsal root ganglion neurons in vitro. J. Physiol. 425:85-115.
34. Thomas, R. C. 1994 Current construction protocol for calcium-sensitive intracellular microelectrodes. J. Physiol. 476:9P.
35. Tsien, R. Y. 1980. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis and properties of prototype structures. Biochemistry. 19:2396-2404.
36. 2+ concentrations. Cell Calcium. 12:29-37.
37. i in intact single fibres from mouse muscle. J. Physiol. 466:611-628.
38. Westerblad, H., and D. G. Allen. 1994. Methods for calibration of fluorescent calcium indicators in skeletal muscle fibres. Biophys. J. 66: 926-927.