Differential regulation of mRNA specific for β1- and β2-adrenergic receptors in human failing hearts. Evaluation of the absolute cardiac mRNA levels by two independent methods

Journal of Molecular and Cellular Cardiology

Volumn 28, Issue 1, 1996, Pages 1-10

  Ihl Vahl, Renate ^a   Eschenhagen, T ^b   Kubler W ^b   Marquetant, R ^b   Nose, M ^b   Schmitz, W ^b   Scholz, H ^b   Strasser, R H ^b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b NONE

Author keywords

Adenylyl cyclase system; Heart failure; mRNA; RNase protection; RT PCR; adrenergic receptors

Indexed keywords

BETA 1 ADRENERGIC RECEPTOR; BETA 2 ADRENERGIC RECEPTOR; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; MESSENGER RNA; RIBONUCLEASE; RNA;

ARTICLE; CONTROLLED STUDY; HEART DILATATION; HEART FAILURE; HEART LEFT VENTRICLE MUSCLE; HEART MUSCLE ISCHEMIA; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR DOWN REGULATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; BLOTTING, SOUTHERN; GENE EXPRESSION REGULATION; HEART FAILURE, CONGESTIVE; HEART TRANSPLANTATION; HUMANS; MIDDLE AGED; MYOCARDIUM; POLYMERASE CHAIN REACTION; RECEPTORS, ADRENERGIC, BETA-1; RECEPTORS, ADRENERGIC, BETA-2; REFERENCE VALUES; RIBONUCLEASES; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 0029988754     PISSN: 00222828     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmcc.1996.0001     Document Type: Article

References (22)

1. AUFFRAY C, ROUGEON F, 1980. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem 17: 303-314.
2. 1-adrenergic receptor gene in cultured ventricular myocytes. J Biol Chem 266: 15863-15869.
3. iα in human hearts with dilated but not ischemic cardiomyopathy. Circulation 82: 1249-1265.
4. BRISTOW MR, GINSBURG R, MINOBE W, CUBICCIOTTI RS, SAGEMAN WS, LURIE K, BILLIGHAM ME, HARRISON DC, STINSON EB, 1982. Decreased catecholamine sensitivity and β-adrenergic-receptor density in failing human hearts. N Engl J Med 307: 205-211.
5. 1-receptor down-regulation in heart failure. Circ Res 59:297-309.
6. 2-adrenergic receptor-mediated adenylate cyclase stimulation in nonfailing and failing human ventricular myocardium. Mol Pharmacol 35:295-303.
7. BRISTOW MR, MINOBE W, RASMUSSEN R, LARRABEE P, SKERL L, KLEIN JW, ANDERSON FL, MURRAY J, MESTRONI L, KARWANDE SV, FOWLER M, GINSBURG R, 1992. β-Adrenergic neuroeffector abnormalities in the failing human heart are produced by local rather than systemic mechanisms. J Clin Invest 89: 803-815.
8. BRISTOW MR, 1993. Changes in myocardial and vascular receptors in heart failure. J Am Coll Cardiol 22: 61A-71A.
9. 1-receptor messenger RNA abundance in the failing human heart. J Clin Invest 92: 2737-2745.
10. 2-Adrenoceptors in the human heart: Properties, function, and alterations in chronic heart failure. Pharmacol Rev 43: 203-242.
11. iα-2 in human endstage heart failure. Circ Res 70: 688-696.
12. FELDMAN AM, CATES AE, VEAZEY WB, HERSHBERGER RE, BRISTOW MR, BAUGHMAN KL, BAUMGARTNER W, VAN DOP C, 1988. Increase of the 40 000-mol wt pertussis toxin substrate (G protein) in the failing human heart. J Clin Invest 82: 189-197.
13. FOWLER MB, LASER JA, HOPKINS GL MINOBE W, BRISTOW MR, 1986. Assessment of the β-adrenergic receptor pathway in the intact failing human heart: Progressive receptor down-regulation and subsensitivity to agonist response. Circulation 74: 1290-1302.
14. 1-adrenergic receptors. J Mol Cell Cardiol 27: 437-452.
15. JONES DT, REED RR, 1987. Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J Biol Chem 262: 14241-14249.
16. KAWASAKI ES, CLARK SS, COYNE MY, SMITH SD, CHAMPLIN R, WITTE ON, MCCORMICK FP, 1988. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc Natl Acad Sci USA 85: 5698-5702.
17. NEUMANN J, SCHOLZ H, DÖRING V, SCHMITZ W, VON MEYERINCK L, KALMAR P, 1988. Increase in myocardial G-proteins in heart failure. Lancet 2: 936-937.
18. 3-adrenergic receptor mRNA in the human brain. Mol Brain Res 29: 369-375.
19. SACHS L, 1978. Angewandte Statistik. Springer-Verlag: Berlin, Heidelberg, New York.
20. STILES GL TAYLOR S, LEFKOWITZ RJ, 1983. Human cardiac β-adrenergic receptors: Subtype heterogeneity delineated by direct radioligand binding. Life Sci 33: 467-473.
21. 2-adrenoceptor mRNA expression in normal and volume overloaded human heart. Cardiovasc Res 25: 737-741.
22. 1-adrenergic receptors in the failing human heart. Circulation 87: 454-463.