Aspartate 203 of the oxaloacetate decarboxylase β-subunit catalyses both the chemical and vectorial reaction of the Na+ pump

EMBO Journal

Volumn 15, Issue 8, 1996, Pages 1842-1849

  Di Berardino, Marco ^a   Dimroth, Peter ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Bacterial Na+ pump; Coupling; Mutagenesis; Transport

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ASPARTIC ACID; BIOTIN; CARBOXYLYASE; MEMBRANE ENZYME; OXALOACETIC ACID;

ARTICLE; CONTROLLED STUDY; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBUNIT; KLEBSIELLA PNEUMONIAE; MEMBRANE TRANSPORT; NONHUMAN; PH; PRIORITY JOURNAL; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS; SODIUM TRANSPORT;

EID: 0029988458     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1996.tb00534.x     Document Type: Article

References (27)

1. Bradford, M.M. (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-251.
2. + pump and its individual subunits in Escherichia coli and analysis of their function Eur. J. Biochem , 231, 790-801.
3. Dimroth, P. (1982a) The role of biotin and sodium in the decarboxylation of oxaloacetate by the membrane-bound oxaloacetate decarboxylase from Klebseilla aerogenes. Eur. J. Biochem., 121, 435-131.
4. +-activated oxaloacetate decarboxylase from Klebsiella aerogenes. Eur. J Biochem., 121, 443-449.
5. Dimroth, P. (1986) Preparation, characterization, and reconstitution of oxaloacetate decarboxylase from Klebsiella aerogenes, a sodium pump. Methods, Enzymol., 125, 530-540.
6. Dimroth, P (1987) Sodium ion transport decarboxylases and other aspects of sodium ion cycling in bacteria. Microbiol Rev , 51, 320-340.
7. + pumps oxaloacetate decarboxylase and methylmalonyl-CoA decarboxylase Biochemistry 23, 5360-5366
8. Dimroth, P. and Thorner, A. (1983) Subunit composition of oxaloacetate decarboxylase and characterization of the α chain as carboxyltransferase Eur. J. Biochem , 137, 107-112.
9. Dimroth, P. and Thomer, A. (1986a) Citrate transport in K.pneumoniae. Biol. Chem. Hoppe-Sevler, 367, 813-823.
10. Dimroth, P. and Thomer, A. (1986b) Kinetic analysis of the reaction mechanism of oxaloacetate decarboxylase from Klebsiella aerogenes. Eur. J. Biochem , 156, 157-162.
11. Dimroth, P and Thomer, A (1992) The sodium ion pumping oxaloacetate decarboxylase of Klebsiella pneumoniae, metal ion content, inhibitors and proteolytic degradation studies. FEBS Lett., 300, 67-70.
12. Dimroth, P. and Thomer, A. (1993) On the mechanism of sodium ion translocation by oxaloacetate decarboxylase of Klebsiella pneumoniae. Biochemistry, 32, 1734-1739.
13. Dunn, J.J. and Studier, F.W. (1983) Complete nucleotide sequence of bacteriophage T7 DNA and the location of T7 genetic elements. J. Mol Biol., 219, 45-59.
14. Huder, B.J. and Dimroth, P (1993) Sequence of the sodium pump methylmalonyl-CoA decarboxylase from Veillonella parvula. J. Biol. Chem , 268, 24564-24571.
15. Laubinger, W. and Dimroth, P (1988) Characterization of the ATP synthase of Propronigenium modestum as a primary sodium pump. Biochemistry, 27, 7531-7537.
16. Laußermair, E , Schwarz, E , Oesterhelt, D., Reinke, H., Beyreuther, K. and Dimroth, P (1989) The sodium ion translocating oxaloacetate decarboxylase of Klebsiella pneumoniae: sequence of the integral membrane-bound subunits β and γ. J Biol. Chem., 264, 14710-14715.
17. Norrandei, J., Kempe, T and Messing, J. (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene, 26, 101-108.
18. +-coupled reversed electron transfer in Klebsiella pneumoniae. Mol. Microbiol., 6, 1943-1948.
19. +-dependent citrate carrier of Klebsiella pneumoniae: evidence for asymmetric orientation of the carrier protein in proteoliposomes Biochemistry, 35, 1018-1026.
20. Sambrook, J., Fritsch, E.F. and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual. 2nd edn Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Sanger, F. Nicklen, S. and Coulson, A.R (1977) DNA sequencing with chain-termination inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
22. Schwarz, E. and Oesterhelt, D (1985) Cloning and expression of K.pneumoniae genes coding for citrate transport and fermentation. EMBO J , 4, 1599-1603.
23. Schwarz, E., Oesterhelt, D., Reinke, H., Beyreuther, K. and Dimroth, P. (1988) The sodium ion translocating oxaloacetate decarboxylase of Klebsiella pneumoniae: sequence of the biotin-containing α-subunit and relationship to other biotin-containing enzymes. J. Biol. Chem., 263, 9640-9645
24. Studier, F.W. and Moffatt, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned series. J. Mol. Biol., 189, 113-130
25. van der Rest, M., Siewe, R.M., Abee, T., Schwarz, E., Oesterhelt, D. and Konings, W.N. (1992) Nucleotide sequence and functional properties of a sodium-dependent citrate transport system from Klebsiella. pneumoniae. J. Biol. Chem., 267, 8971-8976.
26. Woehlke, G., Wifling, K. and Dimroth, P. (1992a) Sequence of the sodium ion pump oxaloacetate decarboxylase from Salmonella typhimurium. J. Biol. Chem., 267, 22798-22803.
27. Woehlke, G., Laußermair, E., Schwarz, E., Oesterhelt, D., Reinke, H., Beyreuther, K. and Dimroth, P. (1992b) Sequence of the β-subunit of oxaloacetate decarboxylase from Klebsiella pneumoniae: a correction of the C-terminal part. J. Biol. Chem , 267, 22804-22805.