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LBL appears to be expressed by all motoneurons whose axons exit in the two major motor nerves (the intersegmental and segmental nerves) and innervate somatic body wall muscles; LBL is probably not expressed by motor axons that exit other nerves and innervate visceral and respiratory muscles.
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7
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13344287835
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note
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The small amount of lbl expression in peripheral sensory neurons begins at stage 14 and disappears by stage early 17.
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8
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13344268378
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note
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Y13 embryos
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9
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13344252612
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note
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A 1.2-kb insert in cDNA clone p3B6 6 was subcloned as six smaller fragments into Bluescript SK+ (Stratagene). Double-stranded template DNA was sequenced on both strands by means of the Autoread sequencing kit (Pharmacia) and ALE automated sequencer (Pharmacia).
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10
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13344255874
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note
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CD37 and CD53, two tetraspanins most closely related to LBL, share 29% amino acid identity with one another but only 19% and 16% amino acid identity, respectively, with LBL. Sequence similarity among tetraspanins is highest in and between TM2 and TM3, in this region, CD37 and CD53 share 39% amino acid identity, whereas LBL shares 27% amino acid identity with CD37 and 25% identity with CD53.
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17
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0027232876
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The 1D4 mAb recognizes Fasciclin II, a marker for motor axons, growth cones, and terminal arbors [D. Van Vactor et al., Cell 73, 1137 (1993)].
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19
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13344291097
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note
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1118) embryos, the RP3 terminal arbor was absent in only 12% of stage late 16 hemisegments (n = 128) and in only 2% of stage early 17 hemisegments (n = 118). Ectopic TN arborizations were observed in 11 % of stage late 16 control embryos (n = 114) and in 3% of stage early 17 control embryos (n = 127).
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20
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0027207209
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This tendency of the TN to sprout onto muscles 6 and 7 in the absence of a functional RP3 synapse has been observed previously [H Keshishian et al., J. Neurobiol. 24, 757 (1993); J. Jarecki and H. Keshishian, J. Neurosci. 15, 8177 (1995).
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This tendency of the TN to sprout onto muscles 6 and 7 in the absence of a functional RP3 synapse has been observed previously [H Keshishian et al., J. Neurobiol. 24, 757 (1993); J. Jarecki and H. Keshishian, J. Neurosci. 15, 8177 (1995).
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(1995)
Blood
, vol.86
, pp. 1348
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Fitter, S.1
Tetaz, T.A.2
Berndt, M.C.3
Ashman, L.K.4
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51
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13344254162
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note
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We thank the Berkeley Drosophila Genome Center for providing mapped P-elements and J. P. Forjanic for suggesting the name late bloomer. C.C.K was supported as a Jane Coffin Childs Postdoctoral Fellow and a Howard Hughes Medical Institute Postdoctoral Associate, G W D. is an American Cancer Society Postdoctoral Fellow, and C.S.G. is an Investigator with HHMI.
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