Improved retrograde axonal transport and subsequent visualization of tetramethylrhodamine (TMR) -dextran amine by means of an acidic injection vehicle and antibodies against TMR

Journal of Neuroscience Methods

Volumn 65, Issue 2, 1996, Pages 157-165

  Kaneko, Takeshi ^a   Saeki, Kazuhiko ^a   Lee, Teffy ^a   Mizuno, Noboru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

(Rat); Antibody; Cortical projection neuron; Dendrite configuration; Dextran amine; Retrograde axonal transport; Tetramethylrhodamine

Indexed keywords

FLUORESCENT DYE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; BRAIN REGION; CONTROLLED STUDY; DENDRITE; HISTOCHEMISTRY; MORPHOMETRICS; NONHUMAN; PRIORITY JOURNAL; RAT; RETROGRADE NERVE FIBER TRANSPORT;

ANIMALS; AXONAL TRANSPORT; BRAIN CHEMISTRY; CEREBRAL CORTEX; DENDRITES; DEXTRANS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FLUORESCENT DYES; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MICROINJECTIONS; NEURAL PATHWAYS; NEURONS; RATS; RATS, WISTAR; RHODAMINES; SPINAL CORD; THALAMUS; VEHICLES;

EID: 0029988068     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(95)00162-X     Document Type: Article

References (22)

1. Behrend, K. and Donicht, M. (1990) Descending connections from the brainstem to the spinal cord in the electric fish Eigemannia: quantitative description based on retrograde horseradish peroxidase and fluorescent-dye transport, Brain Behav. Evol., 35: 227-239.
2. Brandt, H.M. and Apkarian, A.V. (1992) Biotin-dextran: a sensitive anterograde tracer for neuroanatomic studies in rat and monkey, J. Neurosci. Methods, 45: 35-40.
3. Chang, H.T. (1993) Immunoperoxidase labeling of the anterograde tracer Fluoro-Ruby (tetramethylrhodamine-dextran amine conjugate), Brain Res. Bull., 30: 115-118.
4. Dolleman-Van der Weel, M.J., Wouterlood, F.G. and Witter, M.P. (1994) Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine, J. Neurosci. Methods, 51: 9-21.
5. Fritzsch, B. (1993) Fast axonal diffusion of 3000 molecular weight dextran amines, J. Neurosci. Methods, 50: 95-103.
6. Fritzsch, B., Dubuc, R., Ohta, Y. and Grillner, S. (1989) Efferents to the labyrinth of the river lamprey (Lampetra fluviatilis) as revealed with retrograde tracing techniques, Neurosci. Lett., 96: 241-246.
7. Fritzsch, B., Sonntag, R., Dubuc, R., Ohta, Y. and Grillner, S. (1990) Organization of the six motor nuclei innervating the ocular muscles in lamprey, J. Comp. Neurol., 294: 491-506.
8. Gimlich, R.L. and Braun, J. (1985) Improved fluorescent compounds for tracing cell lineage, Dev. Biol., 109: 509-514.
9. Glover, J.C., Petursdottir, G. and Jansen, K.S. (1986) Fluorescent dextran-amines used as axonal tracers in the nervous system of the chicken embryo, J. Neurosci. Methods, 18: 243-254.
10. Haugland, R.P. (1992) Handbook of Fluorescent Probes and Research Chemicals, 5th edn., Molecular Probes, Eugene, OR, 390 pp.
11. Jiang, X.P., Johnson, R.R. and Burkhalter, A. (1993) Visualization of dendritic morphology of cortical projection neurons by retrograde axonal tracing, J. Neurosci. Methods, 50: 45-60.
12. Johnstone, A. and Thorpe, R. (1987) Immunochemistry in Practice, 2nd edn., Blackwell Scientific Publ., Oxford, 306 pp.
13. Kaneko, T. and Mizuno, N. (1988) Immunohistochemical study of glutaminase-containing neurons in the cerebral cortex and thalamus of the rat, J. Comp. Neurol., 267: 590-602.
14. Kaneko, T., Urade, Y. and Mizuno, N. (1988) Correlation between immunochemical characteristics and immunohistochemical applicability of nine lines of monoclonal antibodies against rat brain glutaminase, J. Histochem. Cytochem., 36: 997-1004.
15. Lapper, S.R. and Bolam, J.P. (1991) The anterograde and retrograde transport of neurobiotin in the central nervous system of the rat: comparison with biocytin, J. Neurosci. Methods, 39: 163-174.
16. Nance, D.M. and Burns, J. (1990) Fluorescent dextrans as sensitive anterograde neuroanatomical tracers: applications and pitfalls, Brain Res. Bull., 25: 139-145.
17. Paxinos, G. and Watson, C. (1986) The Rat Brain in Stereotaxic Coordinates, 2nd edn., Academic Press, Sydney, 236 pp.
18. Peters, A. and Kara, D.A. (1985) The neuronal composition of area 17 of rat visual cortex. I. The pyramidal cells, J. Comp. Neurol., 234: 218-241.
19. Schmued, L., Kyriakidis, K. and Heimer, L. (1990) In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextranamine, Fluoro-Ruby, within the CNS, Brain Res., 526: 127-134.
20. Veenman, C.L., Reiner, A. and Honig, M.G. (1992) Biotinylated dextran amine as an anterograde tracer for single- and double-labeling studies, J. Neurosci. Methods, 41: 239-254.
21. Viana, F., Gibbs, L. and Berger, A.J. (1990) Double- and triple-labeling of functionally characterized central neurons projecting to peripheral targets studied in vitro, Neuroscience, 38: 829-841.
22. Wouterlood, F.G. and Jorritsma-Byham, B. (1993) The anterograde neuroanatomical tracer biotinylated dextran-amine: comparison with the tracer Phaseolus uulgaris-leucoagglutinin in preparations for electron microscopy, J. Neurosci. Methods, 48: 75-87.