Characterization and biological significance of sialylα2-3galactosylβ1-4xylosylβ1-(4-methylumbelliferone) synthesized in cultured human skin fibroblasts

Journal of Biochemistry

Volumn 119, Issue 4, 1996, Pages 697-702

  Takagaki, Keiichi ^a   Nakamura, Toshiya ^a   Shibata, Shigeru ^a   Higuchi, Tsuyoshi ^a   Endo, Masahiko ^a  

^a HIROSAKI UNIVERSITY   (Japan)

Author keywords

4 Methylumbelliferyl D xyloside; Cultured human skin fibroblast; Glycosaminoglycan; SA 2 3Gal 1 4Xyl 1 (4 methylumbelliferone)

Indexed keywords

CARBOHYDRATE; CYTIDINE DIPHOSPHATE; GLYCOSAMINOGLYCAN; HYMECROMONE; SIALYLTRANSFERASE;

ARTICLE; CARBOHYDRATE ANALYSIS; DEGRADATION; DIALYSIS; FREEZE DRYING; GEL FILTRATION; GLYCOSAMINOGLYCAN METABOLISM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; PROTON NUCLEAR MAGNETIC RESONANCE; SKIN FIBROBLAST;

EID: 0029987869     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021298     Document Type: Article

References (24)

1. Lindahl, U. and Rodén, L. (1972) Glycoproteins (Gottschalk, A., ed.) pp. 491-517, Elsevier Scientific Publishing, Amsterdam
2. Helting, T. and Rodén, L. (1969) Biosynthesis of chondroitin sulfate. I. Galactosyl transfer in the formation of the carbohydrate-protein linkage region. J. Biol. Chem. 244, 2790-2798
3. Helting, T. and Rodén, L. (1969) Biosynthesis of chondroitin sulfate. II. Glucuronosyl transfer in the formation of the carbohydrate-protein linkage region. J. Biol. Chem. 244, 2799-2805
4. Stoolmiller, A.C., Horwitz, A.L., and Dorfman, A. (1972) Biosynthesis of the chondroitin sulfate proteoglycan. J. Biol. Chem. 247, 3525-3532
5. Malmström, A., Fransson, L.-Å., Höök, M., and Lindahl, U. (1975) Biosynthesis of dermatan sulfate. J. Biol. Chem. 250, 3419-3425
6. Okayama, M., Kimata, K., and Suzuki, S. (1973) The influence of p-nitrophenyl β-D-xyloside on the synthesis of proteochondroitin sulfate by slices of embryonic chick cartilage. J. Biochem. 74, 1069-1073
7. Schwartz, N.B. (1977) Regulation of chondroitin sulfate synthesis. J. Biol. Chem. 252, 6316-6321
8. Robinson, H.C., Brett, M.J., Tralaggan, P.J., Lowther, D.A., and Okayama, M. (1975) The effect of D-xylose, β-D-xylosides and β-D-galactoside on chondroitin sulfate biosynthesis in embryonic chicken cartilage. Biochem. J. 148, 25-34
9. Kato, Y., Kimata, K., Ito, K., Karasawa, K., and Suzuki, S. (1978) Effect of β-D-xyloside and cycloheximide on the synthesis of two type of proteochondroitin sulfate in chick embryo cartilage. J. Biol. Chem. 253, 2784-2789
10. Takagaki, K., Nakamura, T., Kon, A., Tamura, S., and Endo, M. (1991) Characterization of β-D-xyloside-induced glycosaminoglycans and oligosaccharides in cultured human skin fibroblasts. J. Biochem. 109, 514-519
11. Nakamura, T., Izumi, J., Takagaki, T., Shibata, S., Kojima, K., Kato, I., and Endo, M. (1994) A novel oligosaccharide, GlcAβ1-4Xylβ1-(4-methylumbelliferone), synthesized by human cultured skin fibroblasts. Biochem. J. 304, 731-736
12. Shibata, S., Takagaki, K., Nakamura, T., Izumi, J., Kojima, K., Kato, I., and Endo, M. (1995) HNK-1-reactive novel oligosaccharide , sulfate- O-3GlcAβ1 -4Xylβ1-(4-methylumbelliferone), synthesized by cultured human skin fibroblasts. J. Biol. Chem. 270, 13794-13798
13. Izumi, J., Takagaki, K., Nakamura, T., Shibata, S., Kojima, K., Kato, I., and Endo, M. (1994) A novel oligosaccharide, xylosylβ1-4xylosylβ1-(4-methylumbelliferone), synthesized by cultured human skin fibroblasts. J. Biochem. 116, 524-529
14. Freeze, H.H., Sampath, D., and Varki, A. (1993) α- and β-Xylosides alter glycolipid synthesis in human melanoma and chinese hamster ovary cells. J. Biol. Chem. 268, 1618-1627
15. Takagaki, K., Nakamura, T., Kawasaki, H., Kon, A., Ohishi, S., and Endo, M. (1990) Determination of the reducing terminal sugars of glycosaminoglycans using 2-aminopyridine. J. Biochem. Biophys. Methods 21, 209-215
16. Hara, S., Yamaguchi, M., Takemori, Y., Furuhata, K., Ogura, H., and Nakamura, M. (1989) Determination of mono-O-acetylated N-acetylneuraminic acids in human and rat sera by fluorometric high-performance liquid chromatography. Anal. Biochem. 179, 162-166
17. Terho, T.T. and Hartiala, K. (1971) Method for determination of the sulfate content of glycosaminoglycans. Anal. Biochem. 41, 471-476
18. Hess, H.H. and Derr, J.E. (1975) Assay of inorganic and organic phosphorus in the 0.1-5 nanomole range. Anal. Biochem. 63, 607-613
19. Cassidy, J.T., Jourdian, G.W., and Roseman, S. (1965) Thesialic acids. VI. Purification and properties of sialidase from Clostridium perfringens. J. Biol. Chem. 240, 3501-3506
20. Bahl, O.P. and Agarwal, K.M.L. (1969) Glycosidasesof Aspergillus niger. I. Purification and characterization of alpha- and betagalactosidases and beta-N-acetylglucosaminidase. J. Biol. Chem. 244, 2970-2978
21. Oegema, T.R., Jr., Kraft, E.L., Jourdian, G.W., and Van Valen, T.R. (1984) Phosphorylation of chondroitin sulfate in proteoglycans from the swarm rat chondrosarcoma. J. Biol. Chem. 259, 1720-1726
22. Takagaki, K., Kojima, K., Majima, M., Nakamura, T., Kato, I., and Endo, M. (1992) Ion-spray mass spectrometric analysis of glycosaminoglycan oligosaccharides. Glycoconjugate J. 9, 174-179
23. Eppler, C.M., Morré, D.J., and Keenan, T.W. (1980) Ganglioside biosynthesis in rat liver. Biochim. Biophys. Acta 619, 332-343
24. Etchison, J.R., Srikrishna, G., and Freeze, H.H. (1995) A novel method to co-localize glycosaminoglycan-core oligosaccharide glycosyltransferases in rat liver Golgi. J. Biol. Chem. 270, 756-764