Isolation of an IHF-deficient mutant of a Pseudomonas aeruginosa mucoid isolate and evaluation of the role of IHF in algD gene expression

Microbiology

Volumn 142, Issue 10, 1996, Pages 2785-2793

  Delic Attree, Ina ^a   Toussaint, Bertrand ^a   Froger, Alexandra ^a   Willison, John C ^a   Vignais, Paulette M ^a  

^a CEA GRENOBLE   (France)

Author keywords

Alginate; Cystic fibrosis; Integration host factor; Pseudomonas aeruginosa

Indexed keywords

ALGINIC ACID; INTEGRATION HOST FACTOR;

ARTICLE; BINDING SITE; CYSTIC FIBROSIS; ESCHERICHIA COLI; GENE; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; REPORTER GENE; STRUCTURAL GENE;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0029987492     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-142-10-2785     Document Type: Article

References (42)

1. Aviv, M., Giladi, H., Schreiber, G., Oppenheim, A. B. & Glaser, G. (1994). Expression of the genes coding for the Escherichia coli integration host factor are controlled by growth phase, rpoS, ppGpp and by autoregulation. Mol Microbiol 14, 1021-1031.
2. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
3. Chitnis, C. E. & Ohman, D. E. (1993). Genetic analysis of the alginate biosynthetic cluster of Pseudomonas aeruginosa shows evidence of an operonic structure. Mol Microbiol 8, 583-590.
4. Delic-Attree, I., Toussaint, B. & Vignais, P. M. (1995). Cloning and sequence analysis of the genes coding for the integration host factor (IHF) and HU and proteins of Pseudomonas aeruginosa. Gene 154, 61-64.
5. Deretic, V. & Konyecsni, W. M. (1990). A procaryotic regulatory factor with a histone H1-like carboxy-terminal domain: clonal variation of repeats within algP, a gene involved in regulation of mucoidy in Pseudomonas aeruginosa. J Bacteriol 172, 5544-5554.
6. Deretic, V., Gill, J. F. & Chakrabarty, A. M. (1987). Gene algD coding for GDPmannose dehydrogenase is transcriptionally activated in mucoid Pseudomonas aeruginosa. J Bacteriol 169, 351-358.
7. Deretic, V., Dikshit, R., Konyecsni, W. M., Chakrabarty, A. M. & Misra, T. K. (1989). The algR gene, which regulates mucoidy in Pseudomonas aeruginosa, belongs to a class of environmentally responsive genes. J Bacteriol 171, 1278-1283.
8. p1), a histonelike element participating in control of mucoidy in Pseudomonas aeruginosa. J Bacteriol 174, 824-831.
9. Deretic, V., Schurr, M. J., Boucher, J. C. & Martin, D. W. (1994). Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J Bacteriol 176, 2773-2780.
10. DeVault, J. D., Berry, A., Misra, T. K., Darzins, A. & Chakrabarty, A. M. (1989). Environmental sensory signals and microbial pathogenesis: Pseudomonas aeruginosa infection in cystic fibrosis. Bio/ Technology 7, 352-357.
11. DeVault, J. D., Hendrickson, W., Kato, J. & Chakrabarty, A. M. (1991). Environmentally regulated algD promoter is responsive to the cAMP receptor protein in Escherichia coli. Mol Microbiol 5, 2503-2509.
12. Ditton, M. D., Roberts, D. & Weisberg, R. A. (1994). Growth phase variation of integration host factor level in Escherichia coli. J Bacteriol 176, 3738-3748.
13. Freundlich, M., Ramani, N., Mathew, E., Sirko, A. & Tsui, P. (1992). The role of integration host factor in gene expression in Escherichia coli. Mol Microbiol 6, 2557-2563.
14. Gober, J. W. & Shapiro, L. (1992). A developmentally regulated Caulobacter flagellar promoter is activated by 3′ enhancer and IHF binding elements. Mol Biol Cell 3, 913-926.
15. Goldberg, J. B. & Dahnke, T. (1992). Pseudomonas aeruginosa AlgB, which modulates the expression of alginate, is a member of the NtrC subclass of prokaryotic regulators. Mol Microbiol 6, 59-66.
16. E). Proc Natl Acad Sci USA 92, 7941-7945.
17. Higgins, C. F., Hinton, J. C. D., Hulton, C. S. J., Owen-Hughes, T., Pavitt, G. D. & Seirafi, A. (1990). Protein H1: a role for chromatin structure in the regulation of bacterial gene expression and virulence? Mol Microbiol 4, 2007-2012.
18. Hoover, T. R., Santero, E., Porter, S. & Kustu, S. (1990). The integration host factor stimulates interaction of RNA polymerase with NIFA, the transcriptional activator for nitrogen fixation operons. Cell 63, 11-22.
19. Kato, J., Misra, T. K. & Chakrabarty, A. M. (1990). AlgR3, a protein resembling eukaryotic histone H1, regulates alginate synthesis in Pseudomonas aeruginosa. Proc Natl Acad Sci USA 87, 2887-2891.
20. Knutson, C. A. & Jeanes, A. (1968). A new modification of the carbazole analysis: application to heteropolysaccharides. Anal Biochem 24, 470-481.
21. Konyecsni, W. M. & Deretic, V. (1988). Broad-host-range plasmid and M13 bacteriophage-derived vectors for promoter analysis in Escherichia coli and Pseudomonas aeruginosa. Gene 74, 375-386.
22. Krieg, D. P., Helmke, R. J., German, R. F. & Mangos, J. A. (1988). Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophages in vitro. Infect Immun 28, 546-556.
23. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
24. Leitão, J. H. & Sá-Correia, I. (1995). Growth-phase-dependent alginate synthesis, activity of biosynthetic enzymes and transcription of alginate genes in Pseudomonas aeruginosa. Arch Microbiol 163, 217-222.
25. May, T. B. & Chakrabarty, A. M. (1994a). Pseudomonas aeruginosa: genes and enzymes of alginate synthesis. Trends Microbiol 2, 151-157.
26. May, T. B. & Chakrabarty, A. M. (1994b). Isolation and assay of Pseudomonas aeruginosa alginate. Methods Enzymol 235, 295-304.
27. May, T. B., Shinabarger, D., Maharaj, R., Kato, J., Chu, L., DeVault, J. D., Roychoudhury, S., Zielinski, N., Berry, A., Rothmel, R. K., Misra, T. K. & Chakrabarty, A. M. (1991). Alginate synthesis in Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin Microbiol Rev 4, 191-206.
28. Miller, J. H. (1972). In Experiments in Molecular Genetics, pp. 4549-4557. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
29. Mohr, C. D., Martin, D. W., Konyecsni, W. M., Govan, J. R. W., Lory, S. and Deretic, V. (1990). Role of the far-upstream sites of the algD promoter and the algR. and rpoN genes in environmental modulation of mucoidy in Pseudomonas aeruginosa. J Bacteriol 172, 6576-6580.
30. Nash, H. A. (1996). The E. coli HU and IHF proteins: accessory factors for complex protein-DNA assemblies. In Regulation of Gene Expression in Escherichia coli. Edited by E. C. C. Lin & A. S. Lynch. Austin, TX: R. G. Landes (in press).
31. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
32. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
33. Slack, M. P. E. & Nichols, W. W. (1981). The penetration of antibiotics through sodium alginate and through the exopolysaccharide of a mucoid strain of Pseudomonas aeruginosa. Lancet ii, 502-503.
34. Schweizer, H. P. (1992). Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker. Mol Microbiol 6, 1195-1204.
35. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185, 60-89.
36. Toussaint, B., Delic-Attree, I. & Vignais, P. M. (1993a). Pseudomonas aeruginosa contains an IHF-like protein that binds to the algD promoter. Biochem Biophys Res Commun 196, 416-421.
37. Toussaint B., Delic-Attree, I., de Sury d'Aspremont R., David, L., Vinçon, M. & Vignais, P. M. (1993b). Purification of the Integration Host Factor homolog of Rhobacter capsulatus: cloning and sequencing of the hip gene, which encodes the β subunit. J Bacteriol 175, 6499-6504.
38. Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem 218, 97-106.
39. West, S. E. H., Schweizer, H. P., Dall, C., Sample, A. K. & Runyen-Janecky, L. J. (1994). Construction of improved EscherichiaPseudomonas shuttle vectors derived from pUC18/19 and sequence of the region required for their replication in Pseudomonas aeruginosa. Gene 128, 81-86.
40. Wozniak, D. J. (1994). Integration host factor and sequences downstream of the Pseudomonas aeruginosa algD transcription start site are required for expression. J Bacteriol 176, 5068-5076.
41. Wozniak, D. J. & Ohman, D. E. (1991). Pseudomonas aeruginosa AlgB, a two-component response regulator of the NtrC family, is required for algD transcription. J Bacteriol 173, 1406-1413.
42. Wozniak, D. J. & Ohman, D. E. (1993). Involvement of the alginate algT gene and integration host factor in the regulation of the Pseudomonas aeruginosa algB gene. J Bacteriol 175, 4145-4153.