Cloning, direct expression, and purification of a snake venom cardiotoxin in Escherichia coli

Biochemical and Biophysical Research Communications

Volumn 219, Issue 2, 1996, Pages 450-456

  Kumar, T K S ^a   Yang, P W ^a   Lin, S H ^a   Wu, C Y ^b   Lei, B ^c   Lo, S J ^b   Tu, S C ^a,c   Yu, C ^a  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

^c UNIVERSITY OF HOUSTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARDIOTOXIN; SNAKE VENOM; SYNTHETIC DNA;

ARTICLE; CELL INCLUSION; CIRCULAR DICHROISM; CONTROLLED STUDY; ESCHERICHIA COLI; HEMOLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SNAKE; TOXIN ANALYSIS;

EID: 0029987127     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.1996.0254     Document Type: Article

References (22)

1. Dufton, M. J., and Hider, R. C. (1991) in Snake Toxins (E. L. Harvey, Ed.) pp. 259-302, Pergamon press, New York.
2. Bhaskaran, R., Yu, C., and Yang, C. C. (1994) J. Protein Chem. 13, 503-504.
3. Yu, C., Bhaskaran, R., and Yang, C. C. (1994) J. Toxin Toxicol. Rev. 13, 291-315.
4. Yu, C., Lee, C. S., Chaung, L. C., Shei, Y. R., and Wang, C. Y. (1990) Eur. J. Biochem. 193, 789-799.
5. Yu, C., Bhaskaran, R., Chaung, L. C., and Yang, C. C. (1993) Biochemistry 32, 2131-2136.
6. Bhaskaran, R., Haung, C. C., Chang, D. K., and Yu, C. (1994) J. Biol Chem. 269, 23500-23508.
7. Kumar, T.K. S., Lee, C. S., and Yu, C. (1996) in Natural Toxins II (Tu, A. T., and Singh, B. R., Eds.), Plenum press, New York.
8. Chein, K. Y., Chaing, W. N., Jean, J. H., and Wu, W. G. (1991) J. Biol. Chem. 266, 3232-3239.
9. Chiou, S. H., Raynor, R. L., Zheng, B., Chambers, T. C., and Kuo, J. F. (1993) Biochemistry 32, 2062-2067.
10. Desormeaux, A., Laroche, G., Bougis, P. E., and Pezolot, M. (1992) Biochemistry 31, 12173-12182.
11. Bilwes, A., Rees, B., Moras, D., Menez, R., and Menez, A. (1994) J. Mol. Biol. 239, 122-136.
12. Chein, K. Y., Chaing, C. M., Hseu, Y. U., Vyas, A. A., Rule, G. S., and Wu, W. G. (1994) J. Biol. Chem., 269, 14473-14483.
13. Chiou, S. H., Hung, C. C., Haung, H. C., Chen, S. T., Wang, K. T., and Yang, C. C. (1995) Biochem. Biophys. Res. Commun. 206, 22-32.
14. Shaper, E. G. (1986) J. Mol. Biol., 188, 555-564.
15. Chang, H. H., Hu, S. T., Haung, T. F., Chen, S. H., Lee, Y.H. W., and Lo, S. J. (1993) Biochem. Biophys. Res. Commun. 190, 242-249.
16. Lei, B., Liu, M., Hung, S., and Tu, S. -C. (1994) J. Bacteriol. 176, 3552-3558.
17. Laemmli, U. K. (1970) Nature, 227, 680-685.
18. Englander, S. W., and Wand, A. J. (1987) Biochemistry, 26, 5953-5958.
19. Kumar, T.K. S., Jayaraman, G., Lee, C. S., Sivaraman, T., Lin, W. Y., and Yu, C. (1995) Biochem. Biophys. Res. Commun., 207, 536-543.
20. Bhaskaran, R., Haung, C. C., Chang, D. K., and Yu, C. (1994) J. Mol. Biol., 235, 1291-1301.
21. Yeo, M.S. L., Jayaseelan, K., Chung, M.C. M., Gopalakrishnakone, P., Tan, C. H., and Wong, H. A. (1993) Toxicon 31, 53-60.
22. Chi, L. M., Vyas, A. A., Rule, G. S., and Wu, W. G. (1995) Toxicon, 32, 1679-1683.