A double fluorescence staining protocol to determine the cross-sectional area of myofibers using image analysis

Biotechnic and Histochemistry

Volumn 71, Issue 2, 1996, Pages 102-107

  Mozdziak, P E ^a,b   Fassel, T A ^a   Schultz, E ^a   Greaser, M L ^a   Cassens, R G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Muscle Biology Laboratory   (United States)

Author keywords

concanavalin A; cross sectional area; image analysis; myofiber; skeletal muscle; turkey

Indexed keywords

CONCANAVALIN A;

ANIMAL TISSUE; ARTICLE; FLUORESCENCE; IMAGE ANALYSIS; MUSCLE BIOPSY; MUSCLE FIBRIL; NONHUMAN; STAINING; TECHNIQUE; TURKEY (BIRD);

ANIMALIA; AVES; MELEAGRIS GALLOPAVO;

EID: 0029986909     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299609117143     Document Type: Article

References (15)

1. Bader, D., Masaki, T. and Fischman, D. A. 1982. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. J. Cell Biol. 95: 763-770.
2. Graham, S. C., Roy, R. R., West, S. P., Thomason, D. and Baldwin, K. M. 1989. Exercise effects on the size and metabolic properties of soleus fibers in hindlimb-suspended rats. Aviat. Space Environ. Med. 60: 226-234.
3. Jähne, B. 1993. Digital Image Processing Concepts, Algorithms and Scientific Applications. 2nd ed., Springer-Verlag, New York.
4. Luna, L. G. 1968. Manual of Histological Staining Methods of the Armed Forces Insitute of Pathology. 3rd ed. McGraw Hill Book Company, New York, pp. 32-46.
5. Miller, G. R. and Stauber, W. T. 1994. Use of computer-assisted analysis for myofiber size measurements of rat soleus muscles from photographed images. J. Histochem. Cytochem. 42: 377-382.
6. Ott, L. 1988. An Introduction to Statistical Methods and Data Analysis. 3rd Ed. PWS-Kent Publishing Co., Boston, MA.
7. Pena, S. D. J., Gordon, B. B., Karpati, G. and Carpenter, S. 1981. Lectin histochemistry of human skeletal muscle. J. Histochem. Cytochem. 29: 542-546.
8. Rosenblatt, J. D. and Parry, D. J. 1992. Gamma irradiation prevents compensatory hypertrophy of overloaded mouse extensor digitorum longus muscle. J. Appl. Physiol. 73: 2538-2543.
9. Rosenblatt, J. D. and Parry, D. J. 1993. Adaptation of rat extensor digitorum longus muscle to gamma irradiation and overload. Pflüger's Arch. J. Physiol. Europ. J. 423: 255-264.
10. Rosenblatt, J. D., Yong, D and Parry, D. J. 1994. Satellite cell activity is required for hypertrophy of overloaded adult rat muscle. Muscle Nerve 17: 608-613.
11. SAS® Institute 1985. SAS® User's Guide: Statistics. Version 5 edition. SAS Institute Inc., Cary, NC.
12. Stauber, W. T., Fritz, V. K., Burkovskaya, T. E. and Ilyina-Kakueva, E. I. 1992. Effect of spaceflight on the extracellular matrix of skeletal muscle after a crush injury. J. Appl. Physiol. 73 (Suppl.): 74S-81S.
13. Swatland, H. J. 1984. Structure and Development of Meat Animals. Prentice-Hall, Inc., Englewood Cliffs, NJ. pp. 255-311.
14. Swatland, H. J. 1990. A note on the growth of connective tissues binding turkey muscle fibers together. Can. Inst. Food Sci. Technol. J. 23: 239-241.
15. Zukowski, F. and Roels, F. 1994. Automated cytometry of fibre size and spatial distribution in the superficial masseter muscle of the rat at three ages. Histochem. J. 26: 460-467.