Luminometry: A novel bioluminescent immunoassay enhances the quantitation of mucosal and systemic antibody responses

Journal of Immunological Methods

Volumn 190, Issue 2, 1996, Pages 189-197

  Jackson, Raymond J ^a   Fujihashi, Kohtaro ^a   Kiyono, Hiroshi ^a,b   McGhee, Jerry R ^a  

^a UNIVERSITY OF ALABAMA AT BIRMINGHAM   (United States)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Aequorin; AquaLite ; Luminometry; Mucosal immunoassay

Indexed keywords

AEQUORIN; IMMUNOGLOBULIN A;

ANIMAL EXPERIMENT; ANTIBODY DETECTION; ANTIBODY RESPONSE; ARTICLE; BIOLUMINESCENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNIZATION; MOUSE; MUCOSA; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; ARMORACIA RUSTICANA;

EID: 0029986760     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00276-6     Document Type: Article

References (15)

1. Campbell, A.K. and Sala-Newby, G. (1993) Bioluminescent and chemiluminescent indicators for molecular signaling and function in living cells. In: W.T. Mason (Ed.), Fluorescent and Luminescent Probes for Biological Activity. Academic Press, New York, p. 58.
2. 2+ activated photoprotein, aequorin. Photochem. Photobiol. 49, 509.
3. Czerkinsky, C.C., Nilsson, L.A., Nygren, H., Ouchterlony, O. and Tarkowski, A. (1983) A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody secreting cells. J. Immunol. Methods 65, 109.
4. Elson, C.O., Ealding, W. and Lefkowitz, J. (1984) A lavage technique allowing repeated measurement of 1gA antibody in mouse intestinal secretions. J. Immunol. Methods 67, 101.
5. 14C]biotin for kinetic studies and for assay. Biochem. J. 89, 593.
6. Hart, R.C., Scheuer, B.L., Stults, N.L., and Gray, J. (1993) A bioluminescent assay capable of detecting < 2 attomoles of hTSH in serum using the recombinant calcium-triggered photoprotein, aequorin. In: A. Szalay, L. Kricka and P. Stanley (Eds.), Bioluminescence and Chemiluminescence: Status Report. Wiley, Chichester, UK, p. 320.
7. Inouye, S., Noguchi, M., Sakaki, Y., Takagi, Y., Miyata, T., Iwanaga, S., Miyata, T. and Tsuji, F.I. (1985) Cloning and sequence analysis of cDNA for the luminescent protein aequorin. Proc. Natl. Acad. Sci. USA 82, 3154.
8. Jackson, R.J., Fujihashi, K., Xu-Amano, J., Kiyono, H., Elson, C.O. and McGhee, J.R. (1993) Optimizing oral vaccines: induction of systemic and mucosal antibody responses to tetanus toxoid by use of cholera toxin as an adjuvant. Infect. Immun. 61, 4272.
9. Kasten, F.H. (1993) Introduction to fluorescent probes: properties, history and applications. In: W.T. Mason (Ed.), Fluorescent and Luminescent Probes for Biological Activity. Academic Press, New York, p. 12.
10. Schaap, A.P., Akhavan, H. and Romano, L.J. (1989) Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes. Clin. Chem. 35, 1863.
11. Sedgwick, J.D. and Holt, P.G. (1983) A solid-phase immunoenzymatic technique for enumeration of specific antibody-secreting cells. J. Immunol. Methods 57, 301.
12. Shimomura, O. and Johnson, F.H. (1972) Structure of light-emitting moiety of aequorin. Biochemistry. 11, 1602.
13. 2+ indicator in biological systems. Biochem. J. 228, 745.
14. Stults, N.L., Stocks, N.F., Rivera, H., Gray, J., McCann, R.O., O'Kane, D., Cummings, R.D., Cormier, M.J. and Smith, D.F. (1992) Use of recombinant biotinylated aequorin in microtiter and membrane-based assays: purification of recombinant apoaequorin from Escherichia coli. Biochemistry 31, 1433.
15. Zatta, P.F., Nyame, K., Cormier, M.J., Mattox, S.A., Prieto, P.A., Smith, D.F. and Cummings, R.D. (1991) A solid-phase assay for β-1, 4-galactosyltransferase activity in human serum using recombinant aequorin. Anal. Biochem. 194, 185.