4-Pentafluoroethylumbelliferyl-β-D-glucoside as a new fluorogenic substrate for acid β-D-glucosidase

Clinica Chimica Acta

Volumn 248, Issue 2, 1996, Pages 125-133

  Tsvetkova, Inna V ^a   Karpova, Elena A ^a   Dudukina, Tatiana V ^a   Voznyi, Yacov V ^b  

^a INSTITUTE OF BIOMEDICAL CHEMISTRY   (Russian Federation)

^b RUSSIAN ACADEMY OF SCIENCES   (Russian Federation)

Author keywords

fluorigenic substrate; Gaucher's disease; glucosidase

Indexed keywords

4 PENTAFLUOROETHYLUMBELLIFERYL BETA D GLUCOSIDE; BETA GLUCOSIDASE; GLUCOSIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME ACTIVITY; ENZYME SUBSTRATE; FLUOROMETRY; GAUCHER DISEASE; HUMAN; HUMAN CELL; LEUKOCYTE; PRIORITY JOURNAL;

EID: 0029984367     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/0009-8981(95)06246-7     Document Type: Article

References (9)

1. Peters SP, Lee RE, Glew RH. A microassay for Gaucher's disease. Clin Chim Acta 1975;60:391-396.
2. Daniels LB, Glew RH, Diven WF, Lee RE, Radin NS. An improved fluorimetric leukocyte β-glucosidase assay for Gaucher's disease. Clin Chim Acta 1981;115:369-375.
3. Legler G, Liedtke H. Glucosylceramidase from spleen: characterization of its active site with 4-n-alrylumbelliferyl-β-glucosides and N-alkyl derivatives of 1-deoxynojirimycyn. Biol Chem Hoppe-Seyler 1985;366:1113-1122.
4. Voznyi Ya V, Kalicheva IC, Galoyan AA. The synthesis of 4-trifluoromethylumbelliferone glycosides. Bioorg Khim 1989;15:1110-1112.
5. Karpova EA, Voznyi Ya V, Dudukina TV, Tsvetkova IV. 4-Trifluoromethylumbelliferyl glycosides as new substrates for revealing diseases connected with hereditary deficiency of lysosome glycosidases. Biochem Int 1991;24:1135-1144.
6. Bayer V, Pastor R, Cambon AP. Synthesis and spectral study of 4-(perfluoroalkyl)-coumarins. J Fluor Chem 1982;20:187-202.
7. Kolodny EH. General principles and techniques of case identification, carrier testing and prenatal diagnosis. In: Glew RH, Peters SR, eds. Practical enzymology of the sphingolipidoses. New York: AR Liss, 1977;1-38.
8. Goodman C, O'Brein JS. A modified method for the identification of heterozygotes for Gaucher's disease using differential thermal inactivation. Clin Genet 1980;18:226-231.
9. Butcher BA, Gopalan V, Lee RE, Richards TC, Waggoner AS, Glew RH. Use of 4-heptylumbelliferyl-β-D-glucoside to identify Gaucher's disease heterozygotes. Clin Chim Acta 1989;184:235-242.