Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli

Microbiology

Volumn 142, Issue 4, 1996, Pages 1013-1023

  Reverbel Leroy, Corinne ^a   Belaich, Anne ^a   Bernadac, Alain ^a   Gaudin, Christian ^a   Belaich, Jean Pierre ^a,b   Tardif, Chantal ^a,b  

^a CNRS   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Cellulase; Clostridium cellulolyticum; Overproduction; Periplasmic inclusion bodies

Indexed keywords

BACTERIAL DNA; CELLULASE; CELLULOSE; GLUCAN SYNTHASE; MICROCRYSTALLINE CELLULOSE; XYLAN;

AMINO ACID SEQUENCE; ARTICLE; CELL DENSITY; CELL INCLUSION; CLOSTRIDIUM; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOPLASM; DEGRADATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; OSMOTIC STRESS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TEMPERATURE; TIME;

ANGUILLIFORMES; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM CELLULOLYTICUM; ESCHERICHIA COLI;

EID: 0029983647     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-142-4-1013     Document Type: Article

References (49)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol 215, 403-410.
2. Bagnara-Tardif, C., Gaudin, C., Bélaich, A., Hoest, P., Citard, T. & Bélaich, J. P. (1992). Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Gene 119, 17-28.
3. Bélaich, A., Fierobe, H. P., Baty, D., Busetta, B., Bagnara-Tardif, C., Gaudin, C. & Bélaich, J. P. (1992). The catalytic domain of endoglucanase A from Clostridium cellulolyticum, effects of arginine 79 and histidine 122 mutations on catalysis. J Bacteriol 174, 4677-4682.
4. Bernadac, A., Bolla, J. M., Lazdunski, C., Inouye, M. & Pages, J. M. (1987). Precise localization of an overproduced periplasmic protein in Escherichia coli, use of double immuno-gold labelling. Biol Cell 61, 141-147.
5. Bronnenmeier, K., Rucknagel, K. P. & Staudenbauer, W. L. (1991). Purification and properties of a novel type of exo-1,4-β-glucanase (Avicelase II) from the cellulolytic thermophile Clostridium stercorarium. Eur J Biochem 200, 379-385.
6. Creuzet, N., Berenger, J. F. & Frixon, C. (1983). Characterization of exoglucanase and synergistic hydrolysis of cellulose in Clostridium stercorarium. FEMS Microbiol Lett 20, 347-350.
7. Ducros, V., Czjzek, M., Bélaich, A., Gaudin, C., Fierobe, H. P., Bélaich, J. P., Davies, G. J. & Haser, R. (1995). Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5. Structure 3, 939-949.
8. Engebrecht, J., Brent, R. & Kaderbhai, M. A. (1991). In Current Protocols in Molecular Biology, pp. 1·6·1-1·6·10. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. USA: Greene Publishing Associates & John Wiley.
9. Faure, E., Bélaich, A., Bagnara, C., Gaudin, C. & Bélaich, J. P. (1987). Characterization of the cellulolytic system of Clostridium cellulolyticum. In Biomass for Energy and Industry (4th European Communities Conference), pp. 717-721. Edited by G. Grassi, B. Delmon, J. F. Molle & H. Zibetta. London & New York: Elsevier Applied Science.
10. Faure, E., Bélaich A., Bagnara C., Gaudin C. & Bélaich J. P. (1989). Sequence analysis of the Clostridium cellulolyticum endoglucanase-A-encoding gene, celCCA. Gens 84, 39-46.
11. Fierobe, H. P., Gaudin, C., Bélaich, A., Loutfi, M., Faure, E., Bagnara, C., Baty, D. & Bélaich, J. P. (1991). Characterization of endoglucanase A from Clostridium cellulolyticum. J Bacteriol 173, 7956-7962.
12. Fierobe, H. P., Bagnara-Tardif, C., Gaudin, C., Guerlesquin, F., Sauve, P., Bélaich, A. & Bélaich, J. P. (1993). Purification and characterization of endoglucanase C from Clostridium cellulolyticum - catalytic comparison with endoglucanase A. Eur J Biochem 217, 557-565.
13. Fujino, T., Béguin, P. & Aubert, J. P. (1993a). Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein GpA and a protein possibly involved in the attachment of the cellulosome to the cell surface. J Bacteriol 175, 1891-1899.
14. Fujino, T., Karita, S. & Ohmiya, K. (1993b). Nucleotide sequences of the celB gene encoding endo-1,4-β-glucanase-2, ORF1 and ORF2 forming a putative cellulase gene cluster of Clostridium josui. J Ferment Bioeng 76, 243-250.
15. Gerngross, U. T., Romaniec, M. P. M., Kobayashi, T., Huskisson, N. S. & Demain, A. L. (1993). Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. Mol Microbiol 8, 325-334.
16. Giallo, J., Gaudin, C., Bélaich, J. P., Petitdemange, E. & Caillet, F. (1983). Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp. strain H10. Appl Environ Microbiol 45, 843-849.
17. Giallo, J., Gaudin, C. & Bélaich, J. P. (1985). Metabolism and solubilization of cellulose by Clostridium cellulolyticum H10. Appl Environ Microbiol 40, 1216-1221.
18. Grépinet, O., Chebrou, M. C. & Béguin, P. (1988). Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. J Bacteriol 170, 4582-4588.
19. Henrissat, B. & Bairoch, A. (1993). New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293, 781-788.
20. Kruus, K., Lua, A. C., Demain, A. L. & Wu, J. H. D. (1995a). The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. Proc Natl Acad Sci USA 92, 9254-9258.
21. Kruus, K., Wang, W. K., Ching, J. & Wu, J. H. D. (1995b). Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component. J Bacteriol 177, 1641-1644.
22. Lamed, R., Setter, E. & Bayer, E. A. (1983a). Characterization of a cellulose-binding cellulase-containing complex in Clostridium thermocellum. J Bacteriol 156, 828-836.
23. Lamed, R., Setter, E., Kenig, R. & Bayer, E. A. (1983b). The cellulosome, a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities. Biotechnol Bioeng Symp 13, 163-181.
24. LaVallie, E. R., DiBlasio, E. A., Kovacic, S., Grant, K. L., Schendel, P. F. & McCoy, J. M. (1993). A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11, 187-193.
25. Lüthi, E., Bahna Jasmat, N., Grayling, R. A., Love, D. R. & Bergquist, P. L. (1991). Cloning, sequence analysis and expression in Escherichia coli of a gene coding for a β-mannanase from the extremely thermophilic bacterium 'Caldocellum saccharolyticum'. Appl Environ Microbiol 57, 694-700.
26. Madarro, A., Pena, J. L., Lequerica, J. L., Vallès, S., Gay, R. & Flors, A. (1991). Partial purification and characterization of the cellulases from Clostridium cellulolyticum H10. J Chem Technol Biotechol 52, 393-106.
27. Matsushita, O., Russel, J. B. & Wilson, D. B. (1991). A Bacteroides rumicola 1,4-β-D-endoglucanase is encoded in two reading frames. J Bacteriol 173, 6919-6926.
28. Morag, E., Halevy, I., Bayer, E. A. & Lamed, R. (1991). Isolation and properties of a major cellobiohydrolase from the cellulosome of Clostridium thermocellum. J Bacteriol 173, 4155-4162.
29. s (CelS) is synonymous with the major cellobiohydrolase (subunit S8) from the cellulosome of Clostridium thermocellum. Appl Biochem Biotechnol 43, 147-152.
30. Ochman, H., Medhora, M. M., Garza, D. & Hartl, D. L. (1990). Amplification of flanking sequences by inverse PCR. In PCR Protocols, pp. 219-227. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. White. San Diego: Academic Press.
31. Park, J. T. & Johnson, M. S. (1949). A submicrodetermination of glucose. J Biol Chem 181, 149-151.
32. Perlman, D. & Halvorson, H. O. (1983). A putative signal peptidase recognition site and sequence in eucaryotic and procaryotic signal peptides. J Mol Biol 167, 391-409.
33. Petitdemange, E., Caillet, F., Giallo, J. & Gaudin, C. (1984). Clostridium cellulolyticum sp. nov. a cellulolytic mesophilic species from decayed grass. Int J Syst Bacteriol 34, 155-159.
34. Quiviger, B., Franche, C., Lutfalla, G., Rice, D., Haselkorn, R. & Elmerich, C. (1982). Cloning of nitrogen fixation (nif) gene cluster of Azospirillum brasilense. Biochimie 64, 495-502.
35. Roig, V., Fierobe, H. P., Ducros, V., Czjzek, M., Belaich, A., Gaudin, C., Bélaich, J. P. & Haser, R. (1993). Crystallization and preliminary X-ray analysis of the catalytic domain of endoglucanase A from Clostridium cellulolyticum. J Mol Biol 233, 325-327.
36. Salamitou, S., Tokatlidis, K., Béguin, P. & Aubert, J. P. (1992). Involvement of separate domains of the cellulosomal protein S1 of Clostridium thermocellum in binding to cellulose and in anchoring of catalytic subunits to the cellulosome. FEBS Lett 304, 89-92.
37. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Prof Natl Acad Sci USA 74, 5463-5467.
38. Schimming, S., Schwarz, W. H. & Staudenbauer, W. L. (1991). Properties of a thermoactive β-1,3 glucanase (lichenase) from Clostridium thermocellum expressed in Escherichia coli. Biochem Biophys Res Comm 177, 447-452.
39. Shen, H., Tomme, P., Meinke, A., Gilkes, N. R., Kilburn, D. G., Warren, R. A. J. & Miller R. C., Jr (1994). Stereochemical course of hydrolysis catalysed by Cellulomonas fimi CenE, a member of a new family of β-1,4-glucanases. Biochem Biophys Res Commun 199, 1223-1228.
40. Shen, H., Gilkes, H. R., Kilburn, D. G., Miller R. C., Jr & Warren, R. A. J. (1995). Cellobiohydrolase B, a second exo-cellobiohydrolase from the cellulolytic bacterium Cellulomonas fimi. Biochem J 311, 67-74.
41. Shima, S., Igararashi, Y. & Kodama, T. (1991). Nucleotide sequence analysis of the endoglucanase-encoding gene, celCCD, of Clostridium cellulolyticum. Gene 104, 33-38.
42. Shoseyov, O., Takagi, M., Goldstein, M. A. & Doi, R. H. (1992). Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. Proc Natl Acad Sci USA 89, 3483-3487.
43. Stader, J. A. &. Silhavy, T. J (1990). Engineering Escherichia coli to secrete heterologous gene products. Meth Enzymol 185, 166-187.
44. Tokatlidis, K., Salamitou, S., Béguin, P., Dhurjati, P. & Aubert, J. P. (1991). Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components. FEBS Lett 291, 185-188.
45. Totsuka, A. & Fukazawa, C. (1993). Expression and mutation of soybean β-amylase in Escherichia coli. Eur J Biochem 214, 787-794.
46. Wang, W. K., Kruus, K. & Wu, J. H. D. (1993). Cloning and DNA sequence of the gene coding for Clostridium thermocellum cellulase Ss (CelS), a major cellulosome component. J Bacteriol 175, 1293-1302.
47. Wang, W. K., Kruus, K. & Wu, J. H. D. (1994). Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli. Appl Microbiol Biotechnol 42, 346-352.
48. Wood, T. M. (1988). Preparation of crystalline, amorphous, and dyed cellulase substrates. Meth Enzymol 160, 19-23.
49. Wu, J. H. D. & Demain, A. L. (1988). Proteins of the Clostridium thermocellum cellulase complex responsible for degradation of crystalline cellulose. In Biochemistry and Genetics of Cellulose Degradation (FEMS Symposium no. 43), pp. 117-131. Edited by J. P. Aubert, P. Béguin & J. Millet. London & New York: Academic Press.