In vivo interaction between mutated tryptophan repressors of Escherichia coli

Journal of Molecular Biology

Volumn 256, Issue 5, 1996, Pages 889-896

  Storbakk, Norunn ^a   Fenton, Chris ^a   Riise, Hilde M F ^a   Nilsen, Inge W ^a   El Gewely, M Raafat ^a  

^a UNIVERSITY OF TROMSØ   (Norway)

Author keywords

Corepressor binding pocket; In vivo properties of amino acids; Intragenic complementation; Site directed mutagenesis; Tryptophan analogues

Indexed keywords

AMINO ACID; REPRESSOR PROTEIN; TRYPTOPHAN;

AMINO ACID SUBSTITUTION; ARTICLE; ESCHERICHIA COLI; GENE MUTATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; REPRESSOR GENE;

ESCHERICHIA COLI;

EID: 0029983551     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0135     Document Type: Article

References (50)

1. Arrowsmith, C., Pachter, R., Altman, R. & Jardetzky, O. (1991). The solution structures of Escherichia coli trp repressor and trp aporepressor at an intermediate resolution. Eur. J. Biochem. 202, 53-66.
2. Arvidson, D. N., Shapiro, M. & Youderian, P. (1991). Mutant tryptophan aporepressors with altered specificities of corepressor recognition. Genetics, 128, 29-35.
3. Arvidson, D. N., Pfau, J., Hatt, J. K., Shapiro, M., Pecoraro, F. S. & Youderian, P. (1993). Tryptophan super-repressors with alanine 77 changes. J. Biol. Chem. 268, 4362-4369.
4. Arvidson, D. N., Arvidson, C. G., Lawson, C. L., Miner, J., Adams, C. & Youderian, P (1994). The tryptophan repressor sequence is highly conserved among the Enterobacteriaceae. Nucl. Acids Res. 22, 1821-1829.
5. Bass, S., Sorrells, V. & Youderian, P (1988). Mutant trp repressors with new DNA-binding specificities. Science, 242, 240-245.
6. Blaber, M., Zhang, X.-J. & Matthews, B. W. (1993). Structural basis of amino acid α helix propensity. Science, 260, 1637-1640.
7. Boehringer Mannheim (1994). Biochemical Catalogue. pp. 205 and 453, Boehringer Mannheim, Germany.
8. Bolivar, F., Rodriguez, R. L., Greene, P. J., Betlach, M. C., Heynecker, H. L., Boyer, H. W., Crosa, J. H. & Falkow, S. (1977). Construction and characterization of new cloning vehicles II. A multipurpose cloning system. Gene, 2, 95-113.
9. Borden, K. L. B., Beckman, P. & Lane, A. N. (1991). Determination of the orientations of tryptophan analogues bound to the trp repressor and the relationship to activation. (1991). Eur. J. Biochem. 202, 459-470.
10. Chang, A. C. Y. & Cohen, S. N. (1978). Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134, 1141-1166.
11. Chou, W.-Y. & Matthews, K. S. (1989). Serine to cysteine mutations in Trp repressor protein alter tryptophan and operator binding. J. Biol. Chem. 264, 18314-18319.
12. Ebright, R. H., Ebright, Y. W., Pendergast, P. S. & Gunasekera, A. (1990). Conversion of a helix-turn-helix motif sequence-specific DNA binding into a site-specific DNA cleavage agent. Proc. Natl Acad. Sci. USA, 87, 2882-2886.
13. Gibco BRL (1992). Product Catalog, p. 62, European Division, Glasgow, Scotland.
14. Graddis, T. J., Klig, L. S., Yanofsky, C. & Oxender, D. L. (1988). Formation of heterodimers between wild-type and mutant trp aporepressor polypeptides of Escherichia coli. Proteins: Struct. Funct. Genet. 4, 173-181.
15. Grove, C. L. & Gunsalus, R. P. (1987). Regulation of the aroH operon of Escherichia coli by tryptophan repressor. J. Bacteriol. 169, 2158-2164.
16. Gunsalus, R. P. & Yanofsky, C. (1980). Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. Proc. Natl Acad. Sci. USA, 77, 7117-7121.
17. He, J.-J. & Matthews, K. S. (1990). Effect of amino acid alterations in the tryptophan-binding site of the Trp repressor. J. Biol. Chem. 265, 731-737.
18. Heatwole, V. M. & Somerville, R. L. (1991). Cloning, nucleotide sequence and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K12. J. Bacteriol. 173, 108-115.
19. Heatwole, V. M. & Somerville, R. L. (1992). Synergism between the Trp repressor and Tyr repressor in repression of the aroL promoter of Escherichia coli K-12. J. Bacteriol. 174, 331-335.
20. Hurlburt, B. K. & Yanofsky, C. (1993). Analysis of heterodimer formation by the Eschericha coli trp repressor. J. Biol. Chem. 268, 14794-14798.
21. Inoue, H., Nojima, H. & Okayama, H. (1990). High efficiency transformation of E. coli with plasmids. Gene, 26, 23-28.
22. Joachimiak, A., Haran, T. E. & Sigler, P. B. (1994). Mutagenesis supports water mediated recognition in the trp repressor-operator system. EMBO J. 13, 367-372.
23. Kelley, R. L. & Yanofsky, C. (1982). Trp aporepressor production is controlled by autogenous regulation and inefficient translation. Proc. Natl Acad. Sci. USA, 79, 3120-3124.
24. Kelley, R. L. & Yanofsky, C. (1985). Mutational studies with the trp repressor Escherichia coli support the helix-turn-helix model of repressor recognition of operator DNA. Proc. Natl Acad. Sci. USA, 82, 483-487.
25. Klig, L. S., Oxender, D. L. & Yanofsky, C. (1988). Second site revertants of Escherichia coli Trp repressor mutants. Genetics, 120, 651-655.
26. Komeiji, K., Izumi, F., Honda, N., Tsutsui, M., Tamura, T. & Yamato, I. (1994). Glycine 85 of the trp-repressor of E. coli is important in forming the hydrophobic tryptophan binding pocket: experimental and computational approaches. Protein Eng. 7, 1239-1247.
27. Kunkel, T. A., Roberts, J. D. & Zakour, R. A. (1987). Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154, 367-382.
28. Lawson, C. L. & Carey, J. (1993). Tandem binding in crystals of a trp repressor/operator half-site complex. Nature, 366, 178-182.
29. Lawson, C. L. & Sigler, P. B. (1988). The structure of trp pseudorepressor at 1.65 A shows why indole propionate act as a trp inducer. Nature, 333, 869-871.
30. Lehringer, L., Nelson, D. & Micheal, C. (1992). Principles of Biochemistry, 2nd edition, pp. 113, Worth Publisher Inc., USA.
31. Luisi, B. F. & Sigler, P. B. (1990). The stereochemistry and biochemistry of the trp repressor-operator complex. Biochim. Biophys. Acta, 1048, 113-126.
32. Marmorstein, R. Q. & Sigler, P. B. (1989). Stereochemical effects of L-tryptophan and its analogues on trp repressors affinity for operator-DNA. J. Biol. Chem. 264, 9149-9154.
33. Marmorstein, R. Q., Joachimiak, A., Sprinzl, M. & Sigler, P. B. (1987). The structural basis for the interaction between L-tryptophan and the Escherichia coli Trp aporepressor. J. Biol. Chem. 262, 4922-4927.
34. Miller, J. H. (1972). Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
35. Otwinowski, Z., Schevitz, R. W., Zhang, R.-G., Lawson, C. L., Joachimiak, A., Marmorstein, R. Q., Luisi, B. F. & Sigler, P. B. (1988). Crystal structure of the trp repressor/operator complex at atomic resolution. Nature, 335, 321-329.
36. Pabo, C. O. & Sauer, R. T. (1984). Protein-DNA recognition. Annu. Rev. Biochem. 53, 293-321.
37. Pabo, C. O. & Sauer, R. T. (1992). Transcription factors: structural families and principles of DNA recognition. Annu. Rev. Biochem. 61, 1053-1095.
38. Sambrook, J., Fritsh, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, vols 1, 2 and 3. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
39. Sandberg, W. S. & Terwilliger, T. C. (1993). Engineering multiple properties of a protein by combinatorial mutagenesis. Proc. Natl Acad. Sci. USA, 90, 8367-8371.
40. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA-sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5467.
41. Sarsero, J. P., Wookey, P. J. & Pittard, A. J. (1991). Regulation of expression of the E. coli K-12 mtr gene by TyrR Protein and Trp repressor. J. Bacteriol. 173, 4133-4143.
42. Schevitz, R. W., Otwinowski, Z., Joachimiak, A., Lawson, C. L. & Sigler, P. B. (1985). The three-dimensional structure of Trp-repressor. Nature, 317, 782-786.
43. Storbakk, N., Oxender, D. L. & El-Gewely, M. R. (1992). Intragenic complementation between Escherichia coli Trp-repressors with different defects in the tryptophan-binding pocket. Gene, 117, 23-29.
44. Su, T.-Z. & El-Gewely, M. R. (1988). A multisite mutagenesis using T7 DNA polymerase: application for reconstructing a mammalian gene. Gene, 69, 81-91.
45. Sutton, C. L., Mazumder, A., Chen, C. B. & Sigman, D. S. (1993). Transforming the Escherichia coli Trp repressor into a site-specific nuclease. Biochemistry, 32, 4225-4230.
46. Vogel, H. J. & Bonner, D. H. (1956). Acetylornithinase of Escherichia coli: partial purification and some properties. J. Biol. Chem. 218, 97-106.
47. Weichenhan, D. (1991). Fast recovery of DNA from agarose gels by centrifugation through blotting paper. Trends Genet. 7, 109.
48. Yanofsky, C. & Horn, V. (1981). Rifampicin resistance mutations that alter the efficiency of transcription termination at the tryptophan attenuator. J. Bacteriol. 145, 1334-1341.
49. Zhang, R.-G., Joachimiak, A., Lawson, C. L., Schevitz, R. W., Otwinowski, Z. & Sigler, P. B. (1987). The crystal structure of Trp-aporepressor at 1.8 A shows how binding tryptophan enhances DNA affinity Nature, 327, 591-597.
50. Zhao, D., Arrowsmith, C. H., Jia, X. & Jardetzky, O. (1993). Refined solution structures of the Escherichia coli trp holo- and aporepressor. J. Mol. Biol. 229, 735-746.