Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding

Biochemistry

Volumn 35, Issue 22, 1996, Pages 7150-7158

  Gokhale, Rajesh S ^a   Agarwalla, Sanjay ^a   Santi, Daniel V ^b   Balaram, Padmanabhan ^a  

^a INDIAN INSTITUTE OF SCIENCE   (India)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANIDINE; THYMIDYLATE SYNTHASE; UREA;

ARTICLE; CIRCULAR DICHROISM; COVALENT BOND; ENZYME ACTIVE SITE; ENZYME DEGRADATION; ENZYME STABILITY; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN FOLDING;

BINDING SITES; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; FLUORESCENCE; GUANIDINE; GUANIDINES; LACTOBACILLUS CASEI; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR PROBES; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; THYMIDYLATE SYNTHASE; TRYPTOPHAN; UREA;

EID: 0029982719     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi952890e     Document Type: Article

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