High-resolution vertical PAGE: An alternative electrophoretic system with multiple forensic applications

International Journal of Legal Medicine

Volumn 108, Issue 5, 1996, Pages 276-279

  Schneider, Harald R ^a   Rand, Steven ^b  

^a Hessisches Landeskriminalamt   (Germany)

^b UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

AmpFLP STR analysis; Electrophoretic resolution; Singleplex multiple; Vertical PAGE; VNTR polymorphisms

Indexed keywords

DNA;

ARTICLE; DNA SEQUENCE; ELECTROPHORESIS; PRIORITY JOURNAL; TECHNIQUE;

ALLELES; APOLIPOPROTEINS B; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GENE FREQUENCY; HUMANS; MALE; MINISATELLITE REPEATS; PATERNITY; POLYMERASE CHAIN REACTION; RAPE; VAGINAL SMEARS;

EID: 0029982065     PISSN: 09379827     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01369827     Document Type: Article

References (19)

1. Allen RC, Graves G, Budowle B (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques 7:736-744
2. Allen RC, Budowle B, Reeder DJ (1993) Resolution of DNA in the presence of mobility modifying polar and nonpolar compounds by discontinuous electrophoresis on rehydratable polyacrylamide gels. Appl Theor Electrophor 3:173-181
3. Boerwinkle E, Xiong W, Fourest E, Chan L (1989) Rapid typing of repeated hypervanable loci by the polymerase chain reaction: application to the apolipoprotein B 3′ hypervariable region. Proc Natl Acad Sci USA 86:212-216
4. Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen RC (1991) Analysis of the variable number of tandem repeats locus D1S80 by the polymerase chain reaction followed by high resolution polyacrylamide gel electrophoresis. Am J Hum Genet 48:137-144
5. ISFH (1994) Report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphisms in STR (short tandem repeats) systems. Int J Legal Med 107:159-160
6. Kimpton CP, Walton A, Gill P (1992) A further tetranucleotide repeat polymorphism in the vWF gene. Hum Mol Genet 1:287
7. Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods Appl 8(3): 13-22
8. Lorente M, Lorente JA, Wilson MR, Budowle B, Villanueva E (1993) Composite PAGE: an alternate method for increased separation of amplified short tandem repeat alleles. Int J Legal Med 106:69-73
9. Mannucci A, Sullivan KM, Ivanov PL, Gill P (1994) Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin. Int J Legal Med 106:190-193
10. Polymeropoulos MH, Rath DS, Xiao H, Merril CR (1991) Tetranucleotide repeat polymorphism at the human c-fes/fps proto-oncogene (FES). Nucleic Acids Res 19:4018
11. Rand S, Puers C, Skowasch K, Wiegand P, Budowle B, Brinkmann B (1992) Population genetics and forensic efficiency data of four AMPFLPs. Int J Legal Med 104:329-333
12. Sajantila A, Lukka M (1993) Improved separation of PCR amplified VNTR alleles by a vertical polyacrylamide gel electrophoresis. Int J Legal Med 105:355-359
13. Schneider HR (1993) Development of a novel gel system for forensic use. In: Proceedings of the Second International Symposium on the Forensic Aspects of DNA Analysis. FBI Forensic Science Research and Training Center. Quantico, Va, p67
14. Schumm JW, Linns A, Puers C, Sprecher C (1994) Development of nonisotopic multiplex amplification sets for analysis of polymorphic STR loci. In: Proceedings from the fourth International Symposium of Human Identification. Promega. Madison, pp 177-187
15. Sullivan KM, Mannucci A, Kimpton CP, GUI P (1993) A rapid and quantitative DNA sex test: fluorescence-based PCR analysis of x-y homologous gene amelogenin. Biotechniques 15:636-641
16. Urquhart A, Kimpton CP, Downess TJ, Gill P (1994) Variation in short tandem repeat sequences: a survey of 12 microsatellite loci for use as forensic identification markers. Int J Legal Med 107:13-20
17. Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506-513
18. Wiegand P, Schürenkamp M, Schütte U (1992) DNA extraction from mixtures of body fluid using mild preferential lysis. Int J Legal Med 104:359-360
19. Wiegand P, Budowle B, Rand S, Brinkmann B (1993) Forensic validation of the STR systems SE33 and TC11. Int J Legal Med 105:315-320