Generation and characterization of subtype-specific monoclonal antibodies to K+ channel α- and β-subunit polypeptides

Neuropharmacology

Volumn 35, Issue 7, 1996, Pages 851-865

  Bekele Arcuri, Z ^a   Matos, M F ^a   Manganas, L ^a   Strassle, B W ^b   Monaghan, M M ^b   Rhodes, K J ^b   Trimmer, J S ^a  

^a STONY BROOK UNIVERSITY   (United States)

^b PFIZER INC   (United States)

Author keywords

Cortex; Immunoblotting; Immunohistochemistry; Ion channels; Mammalian central nervous system; Transfected cells

Indexed keywords

MONOCLONAL ANTIBODY; POLYPEPTIDE; POTASSIUM CHANNEL;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN REGION; DROSOPHILA; GENE EXPRESSION; GENE LOCATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0029981076     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/0028-3908(96)00128-1     Document Type: Article

References (43)

1. + channel. J. Gen. Physiol. 102:1993;713-28.
2. Chandy K.G. and Gutman G.A. (1995) Voltage-gated potassium channel genes. In Ligand- and Voltage-gated Ion Channels (North R.A., Ed.), pp. 1-71. CRC Press, Boca Raton.
3. Christie M.J., Adelman J.P., Douglass J., North R.A. Expression of a cloned rat brain potassium channel in Xenopus oocytes. Science. 244:1989;221-224.
4. Cook N.S. and Quast U. (1990) Potassium channel pharmacology. In Potassium Channels (Cook N.S. Ed.). Ellis Horwood, Chichester.
5. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucl. Acids Res. 12:1984;387-395.
6. Frech G.C., VanDongen A.M.J., Schuster G., Brown A.M., Joho R.H. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature. 340:1989;642-645.
7. Grupe A., Schroter K.H., Ruppersberg J.P., Stocker M., Drewes T., Beckh S., Pongs O. Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family. Eur. Molec. Biol. Org. J. 9:1990;1749-1756.
8. Hille B. (1992) Ionic Channels of Excitable Membranes. Sinauer Associates, Sunderland, MA.
9. + channels of the Shab subfamily. J. Neurosci. 13:1993;1569-1576.
10. Ikeda S.R., Soler F., Zühlke R.D., Joho R.H., Lewis D.L. Heterologous expression of the human potassium channel Kv2.1 in clonal mammalian cells by direct microinjection of cRNA. Eur. J. Physiol. 422:1992;201-203.
11. Johnson D.A.G., Sportsman J.R., Elder J.H. Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analyt. Tech. 1:1984;3-8.
12. Lipsich L.A., Lewis A.J., Brugge J.S. Isolation of monoclonal antibodies that recognize the transforming proteins of avian sarcoma viruses. J. Virol. 48:1983;352-360.
13. + channel polypeptides in rat hippocampal neurons developing in situ and in vitro. J. Neurosci. 15:1995;3840-3851.
14. + channel β1 gene and functional expression of a β2 gene product. Fedn. Eur. Biochem. Socs. Lett. 370:1995;32-36.
15. McKinnon D. Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family. J. Biol. Chem. 264:1989;8230-8236.
16. + channel containing the alpha subunit Kv1.2 in the basket cell nerve terminals of rat cerebellum. Neuroscience. 57:1993;1039-1045.
17. Mi H., Deerinck T.J., Ellisman M.H., Schwarz T.L. Differential distribution of closely related potassium channels in rat Schwann cells. J. Neurosci. 15:1995;3761-3774.
18. + channel β-subunits with α-subunits. J. Biol. Chem. 271:1996;7084-7089.
19. Paulmichl M., Nasmith P., Hellmiss R., Reed K., Boyle W.A., Nerbonne J.M., Peralta E.G., Clapham D.E. Cloning and expression of a rat cardiac delayed rectifier potassium channel. Proc. Natn. Acad. Sci. U.S.A. 88:1991;7892-7895.
20. Pongs O. Regulation of the activity of voltage-gated potassium channels by β subunits. Sem. Neurosci. 7:1995;137-146.
21. Ramashwami M., Gautam M., Kamb A.A., Rudy B., Tanouye M.A., Mathew M.K. Human potassium channel genes: molecular cloning and functional expression. Molec. Cell. Neurosci. 1:1990;214-223.
22. + channels altered by presence of β-subunit. Nature. 369:1994;289-294.
23. + channel β-subunits: expression and distribution of Kvβ1 and Kvβ2 in adult rat brain. J. Neurosci. 16:1996;4846-4860.
24. + channel α- and β-subunit polypeptides in rat brain. J. Neurosci. 15:1995;5360-5371.
25. + channels conserved in flies, mice and humans. Trends Neurosci. 15:1992;161-166.
26. + channel protein. Evidence from the N-terminal sequence of its larger subunit. J. Biol. Chem. 265:1990;20094-20097.
27. + channels in bovine brain. Biochemistry. 33:1994;1617-1623.
28. + channels from bovine brain. Proc. Natn. Acad. Sci. U.S.A. 91:1994;1637-1641.
29. + channels detected in vivo. Nature. 365:1993;72-75.
30. + channel proteins in rat central neurons. Neuron. 9:1992;271-284.
31. + channel subunits in different neurons of rat brain. J. Neurosci. 14:1994;2408-2417.
32. + channels expressed in transfected mammalian cells. J. Biol. Chem. 269:1994;23204-23211.
33. + channel surface expression through effects early in biosynthesis. Neuron. 16:1996;843-852.
34. Stühmer W., Ruppersberg J.P., Schroter K.H., Sakmann B., Stocker M., Giese K.P., Perschke A., Baumann A., Pongs O. Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain. Eur. Molec. Biol. Org. J. 8:1989;3235-3244.
35. Swanson R., Marshall J., Smith J.S., Williams J.B., Boyle M.B., Folander K., Luneau C.J., Antanavage J., Oliva C., Buhrow S.A., Bennett C., Stein R.B., Kaczmarek L.K. Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain. Neuron. 4:1990;929-939.
36. + channel gene transcription and expression in clonal pituitary cells. Neuron. 11:1993;359-369.
37. + channel cDNAs from human ventricle. Fedn. Am. Soc. Exp. Biol. J. 5:1991;331-337.
38. + channel in rat brain. Proc. Natn. Acad. Sci. U.S.A. 88:1991;10,764-10,768.
39. + fluxes of sea urchin sperm. Cell. 40:1985;697-703.
40. + channels in terminal and juxtaparanodal regions of neurons. Nature. 365:1993;75-79.
41. Wang H., Kunkel D.D., Martin T.M., Schwartzkroin P.A., Tempel B.L. Localization of Kv1.1 and Kv1.2, two K channel proteins, to synaptic terminals, somata, and dendrites in the mouse brain. J. Neurosci. 14:1994;4588-4599.
42. Weiser M., Bueno E., Sekirnjak C., Martone M.E., Baker H., Hillman D., Chen S., Thornhill W., Ellisman M., Rudy B. The potassium channel subunit Kv3.1b is localized to somatic and axonal membranes of specific populations of CNS neurons. J. Neurosci. 15:1995;4298-4314.
43. Yu W., Xu J., Li M. Inactivation of Kv1 potassium channels by the β-subunit, Kvβ1, involves Kvβ1 binding to NAB, a region that mediates subfamily-specific subunit assembly. Neuron. 16:1996;441-453.