Rapid and highly sensitive colloidal silver staining on cellulose acetate membrane for analysis of urinary proteins

Journal of Clinical Laboratory Analysis

Volumn 10, Issue 6, 1996, Pages 403-406

  Hiratsuka, Nobuo ^a   Shiba, Kiyoko ^a   Shinomura, Katsumi ^a   Hosaki, Seijin ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

cellulose acetate membrane; colloidal silver staining; healthy subject; human globulin; human albumin; urinary protein

Indexed keywords

CELLULOSE ACETATE; IMMUNOGLOBULIN; PROTEIN; SERUM ALBUMIN;

ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; ELECTROPHORESIS; HUMAN; PROTEIN ANALYSIS; PROTEIN URINE LEVEL; REPRODUCIBILITY;

ALBUMINURIA; ELECTROPHORESIS, CELLULOSE ACETATE; GAMMA-GLOBULINS; HUMANS; PROTEINS; PROTEINURIA; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN; SILVER STAINING;

EID: 0029979720     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1996)10:6<403::AID-JCLA15>3.0.CO;2-0     Document Type: Article

References (5)

1. Shiba KS, Cho H, Nakao M, Soon PL, Shiba A: An improved method of high-voltage isoelectric focusing on cellulose acetate membranes. Clin Chem 32:2209, 1986.
2. Hiratsuka N, Shiba K, Shinomura K, Hosaki S, Cho H, Nagasaki A, Kobayashi S: Urinary protein fractions in healthy subjects using cellulose acetate membrane electrophoresis followed by staining with Acid Violet17. Biol Pharm Bull 17:1355-1357, 1994.
3. Kovarik A, Ondrkalova M, Prachar J, Stofko J: Rapid and sensitive colloidal silver staining on cellulose acetate membranes. Clin Chem Acta 208:137-139, 1992.
4. Smith EL, Hill RI, Lehman IR, Lefknowitz RJ, Handler P, White A: Principles of Biochemistry New York, McGraw-Hill, 1983, p 50-51.
5. Murata H: Kagaku, Kenshu Shoin, Tokyo, 1994, p 78-79.