Mapping of an autoreactive epitope within glutamate decarboxylase using a diabetes-associated human monoclonal autoantibody and an epitope cDNA library

Hybridoma

Volumn 15, Issue 2, 1996, Pages 103-108

  Richter, Wiltrud ^a   Northemann, Wolfgang ^b   Müller, Marissa ^a   Böhm, Bernhard O ^a  

^a UNIVERSITY OF ULM   (Germany)

^b ELIAS Entwicklungslabor ^*  (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; AUTOANTIBODY; AUTOANTIGEN; COMPLEMENTARY DNA; EPITOPE; GLUTAMATE DECARBOXYLASE; HEAT SHOCK PROTEIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; SYNTHETIC PEPTIDE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CROSS REACTION; DNA LIBRARY; EPITOPE MAPPING; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IMMUNOBLOTTING; INSULIN DEPENDENT DIABETES MELLITUS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STIFF MAN SYNDROME;

EID: 0029979621     PISSN: 0272457X     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.1996.15.103     Document Type: Article

References (17)

1. Meinck HM, Ricker K, Huelser PJ, Schmid E, Pfeiffer J, and Solimena M: Stiff man syndrome: Clinical and laboratory findings in eight patients. J Neurol 1994;241:157-166.
2. Solimena M, Folli F, Aparisi R, Pozza G, and DcCamilli P: Autoantibodies to GABA-ergic neurons and pancreatic beta cells in stiff man syndrome. N Engl J Med 1990;322:1555-1560.
3. Baekkeskov S, Aanstoot HJ, Christgau S, Reetz A, Solimena M, Cascalho M, Folli F, Richter-Olesen H, and DeCamilli P: Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature (London) 1990;347:151-156.
4. Seissler J, Amann J, Mauch L, Haubruck H, Wolfahrt S, Bieg S, Richter W, Holl H, Heinze E, Northemann W, and Scherbaum WA: Prevalence of autoantibodies to the Mr 65,000 and 67,000 isoforms of glutamate decarboxylase (GAD) in insulin-dependent diabetes mellitus. J Clin Invest 1993;92:1394-1399.
5. Richter W, Seissler J, Northemann W, Wolfahrt S, Meinck HM, and Scherbaum WA: Cytoplasmic islet cell antibodies recognize distinct islet antigens in IDDM but not in stiff man syndrome. Diabetes 1993;42:1642-1648.
6. Butler MH, Solimena M, Dirkxx R, Hayday A, and DeCamilli P: Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome. J Exp Med 1993;178:2097-2106.
7. Kim J, Namchuk M, Bugawan T, Fu Q, Jaffe M, Shi Y, Aanstoot HJ, Turck CW, Erlich H, Lennon V, and Baekkeskov S: Higher autoantibody levels and recognition of a linear NH2-terminal epitope in the autoantigen GAD65, distinguish stiff-man-syndrome from insulin-dependent diabetes mellitus. J Exp Med 1994; 180:595-606.
8. Richter W, Endl J, Eiermann TH, Brandt M, Kientsch-Engel R, Thivolet C, Jungfer H, and Scherbaum WA: Human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen. Proc Natl Acad Sci USA 1992;89:8467-8471.
9. Richter W, Shi Y, and Baekkeskov S: Autoreactive epitopes defined by diabetes associated human monoclonal antibodies are localized in the middle and C-terminal domains of the smaller form of glutamate decarboxylase. Proc Natl Acad Sci USA 1993; 90:2832-2836.
10. Berthold H, Frorath B, Scanarini M, Abney CC, Ernst B, and Northemann W: Plasmid pGEX-5T: An alternative system for expression and purification of recombinant proteins. Biotechnol Lett 1992;14:245-250.
11. Frorath B, Abney CC, Scanarini M, Hunt N, and Northemann W: Mapping of a linear autoantigenic epitope within the human thyroid peroxidase using recombinant DNA techniques. J Biochem 1992;111:633-637.
12. School B, and Kaufmann HE: Hydrophobic interaction chromatography for the purification of a mycobacterial heat shock protein of relative molecular mass 60000. J Chromatogr 1991;587:19-23.
13. Siemoneit K, Da Silva Cardoso M, Woelpl A, Koerner K, and Kubanek B: Isolation and epitope characterization of human mon-oclonal antibodies to hepatitis C virus core antigen. Hybridoma 1994;13:9-13.
14. Cianfriglia M, Willingham MC, Tombesi M, Scagliotti GV, Frasca G, and Chersi A: P-glycoprotein epitope mapping. I. Identification of a linear human-specific epitope in the fourth loop of the P-glycoprotein extracellular domain by MM4.17 murine monoclonal antibody to human multi-drug-resistant cells. Int J Cancer 1994; 56:153-160.
15. Jones DB, Coulson AFW, and Duff GW: Sequence homologies between hsp60 and autoantigens. Immunol Today 1993;14:115-118.
16. Schmitt HD, Wagner P, Pfaff E, and Gallwitz D: The ras related YPTI gene product in yeast: A GTP binding protein that might be involved in microtubule organization. Cell 1986;47:401-412.
17. Li L, Hagopian WA, Brashear HR, Daniels T, and Lernmark A: Identification of autoantibody epitopes of glutamic acid decarboxylase in stiff-man syndrome patients. J Immunol 1994; 152:930-934.