A recombinant Escherichia coli heat-stable enterotoxin b (STb) fusion protein eliciting neutralizing antibodies

FEMS Immunology and Medical Microbiology

Volumn 13, Issue 4, 1996, Pages 317-323

  Dubreuil, J Daniel ^a   Letellier, Ann ^a   Harel, Josée ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Biological activity; Escherichia coli; Fusion protein; Neutralizing antibody; STb enterotoxin

Indexed keywords

BACTERIAL VACCINE; ESCHERICHIA COLI ENTEROTOXIN; HYBRID PROTEIN; NEUTRALIZING ANTIBODY;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DRUG ACTIVITY; ESCHERICHIA COLI; IMMUNIZATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; RABBIT; TOXICITY TESTING;

EID: 0029979120     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/0928-8244(96)00009-0     Document Type: Article

References (25)

1. Söderlind, O., Thafvelin, B. and Möllby, R. (1988) Virulence factors in Escherichia coli strains isolated from Swedish piglets with diarrhoea. J. Clin. Microbiol. 26, 879-884.
2. Staples, S.J., Asher, S.E. and Giannella, R.A. (1980) Purification and characterization of heat-stable enterotoxin produced by a strain of E. coli pathogenic for man. J. Biol. Chem. 255, 4716-4721.
3. Lallier, R., Bernard, F., Gendreau, M., Lazure, C., Seidah, N.G. Chrétien, M. and St-Pierre, S.A. (1982) Isolation and purification of Escherichia coli heat-stable enterotoxin of porcine origin. Ann. Biochem. 127, 267-275.
4. Fujii, Y., Hayashi, M., Hitotsubashi, S., Fuke, Y., Yamanaka, M. and Okamoto, K. (1991) Purification and characterization of Escherichia coli heat-stable enterotoxin II. J. Bacteriol. 173, 5516-5522.
5. Handl, C.E., Harel, J., Flock, J.-I. and Dubreuil, J.D. (1993) High yield of active STb enterotoxin from a fusion protein (MBP-STb) expressed in Escherichia coli. Prot. Express. Purific. 4, 275-281.
6. Whipp, S.C. (1987) Protease degradation of Escherichia coli heat-stable, mouse-negative, pig-positive enterotoxin. Infect. Immun. 55, 2057-2060.
7. Fairbrother, J.M. (1992) Enteric colibacillosis. In: Diseases of Swine (Leman, A.D., Straw, B.E., Mengeling, W.L., D'Allaire, S. and Taylor, D.J., Eds.), Iowa State University Press, Ames.
8. Harel, J., Lapointe, H., Fallara, A., Lortie, L.A., Bigras-Poulin, M., Larivière, S. and Fairbrother, J.M. (1991) Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhoea. J. Clin. Microbiol. 29, 745-752.
9. Moon, H.W. and Bunn, T.O. (1993) Vaccines for preventing enterotoxigenic Escherichia coli infections in farms animals. Vaccine 11, 213-220.
10. Dubreuil, J.D., Fairbrother, J.M., Lallier, R. and Larivière, S. (1991) Production and purification of heat-stable enterotoxin b from a porcine Escherichia coli strain. Infect. Immun. 58, 198-203.
11. Handl, C.E. and Flock, J.-I. (1990) Studies on heat-stable enterotoxin type II (STII or STb) from Escherichia coli. In: Molecular Pathogenesis of Gastrointestinal Infections (Wadström, T., Mäkelä, P.H., Svennerholm, A.-M. and Wolf-Watz, H., Eds.), pp. 307-311, Plenum, New York.
12. Busque, P., Letellier, A. and Dubreuil, J.D. (1995) Production of Escherichia coli STb enterotoxin is subject to catabolite repression. Microbiology. 141, 1621-1627.
13. Lawrence, R.M., Huang, P.T., Glick, J., Oppenheim, J.D. and Maas, W.K. (1990) Expression of the cloned gene for enterotoxin STb of Escherichia coli. Infect. Immun. 58, 970-977.
14. Urban, R.G., Piper, E.M., Dreyfus, L.A. and Whipp, S. (1990) High-level production of Escherichia coli STb heat-stable enterotoxin and quantification by a direct enzyme-linked immunoabsorbent assay. J. Clin. Microbiol. 28, 2383-2388.
15. Whipp, S.C. Moseley, S.L. and Moon, H.W. (1986) Microscopic alterations in jejunal epithelium of 3-week-old pigs induced by pig-specific, mouse-negative, heat-stable Escherichia coli enterotoxin. Am. J. Vet. Res. 47, 615-618.
16. Maina, C.V., Riggs, P.D., III Grandea, A.G., Slatko, B.E., Moran, L.S., Tagliamonte, J.A., McReynolds, L.A. and Guan, C. (1988) A vector to express and purify foreign proteins in Escherichia coli by fusion to, and separation from maltose binding protein. Gene 74, 365-373.
17. Duplay, P., Bédouelle, H. B., Fowler, A., Zabin, I., Saurin, W. and Hofnung, M. (1984) Sequences of the mal E gene and of its product, the maltose-binding protein of Escherichia coli K12. J. Biol. Chem. 259, 10606-10613.
18. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982). In: Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
19. Bossé, M., Handl, C.E., Lortie, L.-A., Harel, J. and Dubreuil, J.D. (1993) Fusion of the genes encoding Escherichia coli heat-stable enterotoxin b (STb) and the maltose-binding protein to obtain mature STb enterotoxin. J. Gen. Microbiol. 139, 631-638.
20. Lee, C.H., Moseley, S.L., Moon, H.W., Whipp, S.C., Gyles, C.L. and So, M. (1983) Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enteropathogenic Escherichia coli heat-stable toxin II producers. Infect. Immun. 42, 264-268.
21. 4. Nature (Lond.) 227, 680-685.
22. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 74, 4350-4354.
23. Handl, C., Rönnberg, B., Nilsson, B., Olsson, E., Jonsson, H., and Flock, J.I. (1988) Enzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin type II. J. Clin. Microbiol. 26, 1555-1560.
24. Clements, J.D. (1990) Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heat-labile and heat-stable enterotoxins. Infect. Immun. 58, 1159-1166.
25. Löwenadler, B., Lake, M., Elmblad, A., Holmgren, E., Holmgren, J., Karlstrom, A. and Svennerholm, A.-M. (1991) A recombinant Escherichia coli heat-stable enterotoxin (STa) fusion protein eliciting anti-STa neutralizing antibodies. FEMS Microbiol. Lett. 82, 271-278.