In vitro UVB irradiation of bovine crystalline lens causes cell damage and reduction in leucine aminopeptidase activity in lens epithelium

Journal of Photochemistry and Photobiology B: Biology

Volumn 32, Issue 1-2, 1996, Pages 81-87

  Stuart, D D ^a,b   Doughty, M J ^a,c  

^a UNIVERSITY OF WATERLOO   (Canada)

^b UNIVERSITY OF ALBERTA   (Canada)

^c GLASGOW CALEDONIAN UNIVERSITY   (United Kingdom)

Author keywords

Bovine lens; Epithelium; Leucine aminopeptidase; UVB

Indexed keywords

CYTOSOL AMINOPEPTIDASE;

ANIMAL TISSUE; ARTICLE; CATTLE; CELL DAMAGE; CONTROLLED STUDY; ENZYME REPRESSION; HISTOLOGY; LENS; LENS EPITHELIUM; NONHUMAN; OPTICS; ORGAN CULTURE; PRIORITY JOURNAL; RADIATION INJURY; ULTRAVIOLET B RADIATION;

EID: 0029978439     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/1011-1344(95)07193-8     Document Type: Article

References (31)

1. F.H. Verhoeff and L. Bell, The pathological effects of radiant energy on the eye, Proc. Am. Acad. Arts Sci., 51 (1916) 628-759.
2. W.S. Duke-Elder and P.M. Duke-Elder, A histological study of the action of short-wave light upon the eye with a note on "inclusion bodies", Br. J. Ophthalmol., 13 (1929) 1-37.
3. J.G. Jose and D.G. Pitts, Wavelength dependency of cataracts in albino mice following chronic exposures, Exp. Eye Res., 41 (1985) 545-563.
4. J. Schmidt, C. Schmitt, A. Wegener and O. Hockwin, Histological changes of rat lenses after UV-irradiation and D-penicillamine treatment, Ophthalmic Res., 25 (1993) 69-76.
5. J. Schmidt, C. Schmitt, M. Kojima and O. Hockwin, Biochemical and morphological changes in rat lenses after long-term UVB irradiation, Ophthalmic Res., 24 (1992) 317-325.
6. P.O. Söderberg, Acute cataract in the rat after exposure to radiation in the 300 nm wavelength region, Acta Ophthalmol., 66 (1988) 141-152.
7. D.G. Pitts, A.P. Cullen and P.D. Hacker, Ocular effects of ultraviolet radiation from 295 to 365 nm, Invest. Ophthalmol. Vis. Sci., 16 (1977) 932-939.
8. A.P. Cullen, Additive effects of ultraviolet radiation, Am. J. Optom. Physiol. Optics, 57 (1980) 808-814.
9. A. Bachem, Ophthalmic ultraviolet action spectra, Am. J. Ophthalmol., 41 (1956) 969-975.
10. F.M. Barker, The transmittance of the electromagnetic spectrum from 200 nm to 2500 nm through the optical tissues of the eye of the pigmented rabbit, MSc Thesis, University of Houston, Texas, 1979.
11. D.G. Pitts, Ocular effects of radiant energy, in Environmental Vision. Interactions of the Eye, Vision and the Environment, Butterworth-Heinemann, Toronto, 1993, pp. 151-220.
12. P.G. Söderberg, J.P.G. Bergmanson, B.T. Phillipson and B.M. Tengroth, A new model for in vitro investigation of acute response in the UV radiation exposed lens, Acta Ophthalmol., 68 (Suppl. 196) (1990) 221-228.
13. D.D. Stuart, J.G. Sivak, A.P. Cullen, J.A. Weerheim and C.A. Monteith, UVB radiation and the optical properties of cultured bovine lenses, Curr. Eye Res., 10 (1991) 177-184.
14. D.D. Stuart, A.P. Cullen, J.G. Sivak and M.J. Doughty, Optical effects of UVA and UVB radiation on the cultured bovine lens, Curr. Eye Res., 13 (1994) 371-376.
15. K. Hightower and J. McCready, Comparative effect of UVA and UVB on cultured rabbit lens, Photochem. Photobiol., 58 (1993) 827-830.
16. D.Y. Li and R.F. Borkman, Photodamage to calf lenses in vitro by excimer laser radiation at 308, 337 and 350 nm, Invest. Ophthalmol. Vis. Sci., 31 (1990) 2180-2184.
17. S.M. Clarke, M.J. Doughty and A.P. Cullen, Acute effects of ultraviolet-B irradiation on the corneal surface of the pigmented rabbit studied by quantitative scanning electron microscopy, Acta Ophthalmol., 68 (1990) 639-650.
18. M. Iwig and D. Glaesser, Investigations on mitotic activity, cell density, and enzyme activities in the bovine eye lens during cell differentiation, Ophthalmic Res., 4 (1972/1973) 328-342.
19. K. Hightower and J. McCready, Physiological effects of UVB irradiation on cultured rabbit lens, Invest. Ophthalmol. Vis. Sci., 33 (1992) 1783-1787.
20. J.B. Wolff and R.A. Resnik, Aminopeptidase of the ocular lens. II. Substrate specificity, Biochim. Biophys. Acta, 73 (1963) 613-622.
21. A. Taylor, M. Daims, J. Lee and T. Surgenor, Identification and quantification of leucine aminopeptidase in aged normal and cataractous human lenses and ability of bovine lens LAP to cleave bovine crystallins, Curr. Eye Res., 2 (1982/1983) 47-56.
22. A. Taylor, M.J. Brown, M.A. Dalms and J. Cohen, Localization of leucine aminopeptidase in normal hog lens by immunofluorescence and activity assays, Invest. Ophthalmol. Vis. Sci., 24 (1983) 1172-1180.
23. J. Blondin and A. Taylor, Measures of leucine aminopeptidase can be used to anticipate UV-induced age-related damage to lens proteins: ascorbate can delay this damage, Mech. Ageing Dev., 41 (1987) 39-46.
24. M.J. Doughty, K.L. Herbert, D.D. Stuart and J.G. Sivak, Use of corneal dimensions to standardise selection of the crystalline lens from bovine slaughterhouse eyes in the lens optical function toxicity test, In Vitro Toxicol., accepted for publication.
25. O.H. Lowry, N.J. Rosebrough, A.L. Farr and R.J. Randall, Protein measurement with the folin phenol reagent, J. Biol. Chem., 193 (1961) 265-275.
26. J.L. Bailey, Estimation of protein, in Techniques in Protein Chemistry, Prentice-Hall, New York, 1967, pp. 340-341.
27. J.G. Sivak, D. Gershon, A. Dovrat and J. Weerheim, Computer assisted scanning laser monitor of optical quality of the excised crystalline lens, Invest. Opthalmol. Vis. Sci., 24 (1986) 1162-1166.
28. N. Ledeme, O. Vincent-Fiquet, G. Hennon and R. Plaquet, Human liver L-leucine aminopeptidase: evidence for two forms compared to pig enzyme, Biochimie, 65 (1983) 397-404.
29. H. Agematu, T. Nakashima, N. Shibamoto, T. Yoshioka, T. Shin and S. Murao, Colorimetric measurement of total activity of leucine aminopeptidase and arylamidase in serum using laccase-catalyzed oxidative decarboxylation, J. Ferment. Bioeng., 77 (1994) 479-482.
30. M.J. Doughty and D.D. Stuart, unpublished, 1995.
31. K.R. Hightower, J.R. Reddan, J.P. McCready and D.C. Dziedzic, Lens epithelium: a primary target of UVB irradiation, Exp. Eye Res., 59 (1994) 557-564.