The expressed protein in glyphosate-tolerant soybean, 5-enolypyruvylshikimate-3-phosphate synthase from Agrobacterium sp. strain CP4, is rapidly digested in vitro and is not toxic to acutely gavaged mice

Journal of Nutrition

Volumn 126, Issue 3, 1996, Pages 728-740

  Harrison, Leslie A ^a   Bailey, Michele R ^a   Naylor, Mark W ^a   Ream, Joel E ^a   Hammond, Bruce G ^a   Nida, Debbie L ^a   Burnette, Barry L ^a   Nickson, Thomas E ^a   Mitsky, Timothy A ^a   Taylor, Mary L ^a   Fuchs, Roy L ^a   Padgette, Stephen R ^a  

^a MONSANTO COMPANY   (United States)

Author keywords

5 enolypyruvylshikimate 3 phosphate synthase; Glyphosate; Mice; Soybean

Indexed keywords

AGROBACTERIUM SP.; ANIMALIA; GLYCINE MAX; MAMMALIA;

3 PHOSPHOSHIKIMATE 1 CARBOXYVINYLTRANSFERASE; GLYPHOSATE; VEGETABLE PROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEEDING; GENETIC ENGINEERING; GENETIC MANIPULATION; MOUSE; NONHUMAN; NUTRITION; RHIZOBIUM; SAFETY; TRANSGENIC PLANT;

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE; ALKYL AND ARYL TRANSFERASES; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; BRASSICA; DIGESTION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, PLANT; GLYCINE; GOSSYPIUM; HERBICIDES; MALE; MICE; MICE, MUTANT STRAINS; MOLECULAR SEQUENCE DATA; RHIZOBIUM; SEQUENCE HOMOLOGY, AMINO ACID; SOYBEANS; TRANSFERASES;

EID: 0029978074     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/126.3.728     Document Type: Article

References (53)

1. Akeson, W. R. & Stahmann, M. A. (1964) A pepsin pancreatin digest index of protein quality evaluation. J. Nutr. 83: 257-261.
2. Alam, A. S., Hagerman, L. M. & Imondi, A. R. (1980) Bioavailability of sulpiride tablet and capsule in dogs. Arch. Int. Pharmodyn. Ther. 247: 180-189.
3. Bairoch, A. & Boeckmann B. (1994) The SWISS-PROT protein sequence data bank: current status. Nucleic Acids Res. 22: 3578-3580.
4. Barry, G., Kishore, G., Padgette, S., Taylor, M., Kolacz, K., Weldon, M., Re, D., Eichholtz, D., Fincher, K. & Hallas, L. (1992) Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to crop plants. In: Biosynthesis and Molecular Regulation of Amino Acids in Plants (Singh, B. K., Flores, H. E. & Shannon, J. C., ed.), pp. 139-145. American Society of Plant Physiologists, Rockville, MD.
5. Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
6. Breslow, N. (1970) A generalized Kruskal-Wallis test for comparing K-samples subject to unequal patterns of censorship. Biometrika 57: 579-594.
7. Burkes, W. & Fuchs, R. L. (1996) Assessment of the endogenous allergens in glyphosate-tolerant and commercial soybean varieties. J. Allergy Clin. Immunol. (in press).
8. C.A.B. International (1991) In Vitro Digestion for Pigs and Poultry. C.A.B. International, Wallingford, Oxon, U.K.
9. Davenport, H. W. (1971) Physiology of the Digestive Tract, 3rd ed. Year Book Medical Publishers, Chicago, IL.
10. Dayhoff, M. O. (1978) Atlas of Protein Sequence and Structure. National Biomedical Research Foundation, Washington, DC.
11. della-Cioppa, G., Bauer, S. C., Klein, B. K., Shah, D. M., Fraley, R. T. & Kishore, G. (1986) Translocation of the precursor of 5-enolpyruvylshikimate-3-phosphate synthase into chloroplasts of higher plants in vitro. Proc. Natl. Acad. Sci. U.S.A. 83: 6873-6877.
12. Dixon, W. J. & Massey, F. J., Jr. (1969) Introduction to Statistical Analysis, 3rd ed. McGraw-Hill, New York, NY.
13. Doherty, A. M., Kaltenbronn, J. S., Hudspeth, J. P., Repine, J. T., Roark, W. H., Sircar, I., Tinney, F. J., Connolly, C. J., Hodges, J. C., Taylor, M. D., Batley, B. L., Ryan, M. J., Essenburg, A. D., Rapundalo, S. T., Weishaar, R. E., Humblet, C. & Lunney, E. A. (1991) New inhibitors of human renin that contain novel replacements at the P2 site. J. Med. Chem. 34: 1258-1271.
14. Draper, N. R. & Smith, H. (1966) Applied Regression Analysis. Wiley, New York, NY.
15. Dunnett, C. W. (1994) A multiple comparison procedure for comparing several treatments with a control. J. Am. Stat. Assoc. 50: 1096-1121.
16. Fuchs, R. L., Ream, J. E., Hammond, B. G., Naylor, M. W., Leimgruber, R. M. & Berberich, S. A. (1993) Safety assessment of the neomycin phosphotransferase II (NPTII) protein. BioTechnology 11: 1543-1547.
17. George, D. G., Barker, W. C. & Hunt, L. T. (1990) Mutation data matrix and its uses. Methods Enzymol. 183: 333-351.
18. Gibskov, M. & Devereux, J. (1992) Sequence Analysis Primer. W. H. Freeman and Co., New York, NY.
19. Hammond, B. G., Vicini, J. L., Hartnell, G. F., Naylor, M. W., Knight, C. D., Robinson, E. H., Fuchs, R. L. & Padgette, S. R. (1996) The feeding value of soybeans fed to rats, chickens, catfish and dairy cattle is not altered by incorporation of glyphosate tolerance. J. Nutr. 126: 717-727.
20. Haslam, E. (1993) Shikimic Acid: Metabolism and Metabolites. John Wiley and Sons, Chichester, England.
21. Health Science Computing Facility (1977) BMDP Biomedical Computer Programs P Series Manual. University of California Press, Los Angeles, CA.
22. Hollander, M. & Wolfe, D. A. (1973) Nonparametric Statistical Methods. Wiley, New York, NY.
23. Hunkapiller, M. W., Hewick, R. M., Dreyer, R. J. & Hood, L. E. (1983) High-sensitivity sequencing with a gas-phase sequenator. Methods Enzymol. 91: 399-413.
24. Jones, D. D. & Maryanski, J. H. (1991) Safety considerations in the evaluation of transgenic plants for human foods. In: Risk Assessment in Genetic Engineering (Levin, M. A. & Strauss, H. S., ed.) pp. 64-82. McGraw-Hill, New York, NY.
25. Klee, H. J., Muskopf, Y. M. & Gasser, C. S. (1987) Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants. Mol. & Gen. Genet. 210: 437-442.
26. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.) 227: 680-685.
27. Mann, H. B. & Whitney, D. R. (1947) On a test of whether one of two random variables is stochastically larger than the other. Ann. Math. Stat. 18: 50-60.
28. Marquez, U.M.L. & Lajolo, F. M. (1981) Composition and digestibility of albumins, globulins, and glutelins from Phaseolus vulgaris. J. Agric. Food. Chem. 29: 1068-1074.
29. McCallum, R. W. (1989) Motor function of the stomach in health and disease. In: Gastrointestinal Disease; Pathophysiology Diagnosis Management vol. 1, 4th ed. (Sleisenger, M. H. and Fordtran, J. S., eds.), pp. 685-689. W. B. Saunders, Philadelphia, PA.
30. National Research Council (1985) Guide for the Care and Use of Laboratory Animals. Publication no. 85-23 (rev.), National Institutes of Health, Bethesda, MD.
31. Needleman, S. B. & Wunsch, C. P. (1970) A general method applicable to the search for similarities in the amino acid sequence of two proteins. J. Mol. Biol. 48: 443-453.
32. Nielson, S. S. (1988) Degradation of bean proteins by endogenous and exogenous proteases-a review. Cereal Chem. 65: 435-442.
33. Padgette, S. R., Huynh, Q. K., Aykent, S., Sammons, R. D., Sikorski, J. A. & Kishore, G. M. (1988) Identification of the reactive cysteines of Escherichia coli 5-enolypyruvylshikimate-3-phosphate synthase and their nonessentiality for enzymatic catalysis. J. Biol. Chem. 263: 1798-1802.
34. Padgette, S. R., Huynh, Q. K., Borgmeyer, J., Shah, D. M., Brand, L. A., Re, D. B., Bishop, B. F., Rogers, S. G., Fraley, R. T. & Kishore, G. M. (1987) Bacterial expression and isolation of Petunia hybrida 5-enolpyruvyl-shikimate-3-phosphate synthase. Arch. Biochem. Biophys. 258: 564-573.
35. Padgette, S. R., Kolacz, K. H., Delannay, X., Re, D. B., LaVallee, B. J., Tinius, C. N., Rhodes, W. K., Otero, Y. I., Barry, G. F., Eichholtz, D. A., Peschke, V. M., Nida, D. L., Taylor, N. B. & Kishore, G. M. (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci. 35: 1451-1461.
36. Padgette, S. R., Re, D. B., Barry, G. F., Eichholtz, D. E., Delannay, X., Fuchs, R. L., Kishore, G. M. & Fraley, R. T. (1996a) New weed control opportunities: development of soybeans with a Roundup Ready™ gene. In: Herbicide Resistant Crops (Duke, S.O., ed.), pp. 53-84. CRC Press, Boca Raton, FL.
37. Padgette, S. R., Taylor, N. B., Nida, D. L., Bailey, M. R., MacDonald, J., Holden, L. R. & Fuchs, R. L. (1996b) The composition of glyphosate-tolerant soybeans is equivalent to that of conventional soybeans. J. Nutr. 126: 702-716.
38. Pariza, M. W. & Foster, E. M. (1983) Determining the safety of enzymes used in food processing. J. Food Prot. 46: 453-468.
39. Pearson, W. R. (1990) Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 183: 63-98.
40. Pearson, W. & Lipman, D. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. U.S.A. 85: 2444-2448.
41. Sjoblad, R. D., McClintock, J. T. & Engler, R. (1992) Toxicological considerations for protein components of biological pesticide products. Regul. Toxicol. Pharmacol. 15: 3-9.
42. Slater, R. J. (1988) The expression of foreign DNA in Escherichia coli. In: Molecular Biology and Biotechnology (Walker, J. M. & Gingold, E. B., ed.), pp. 47-67. Burlington House, London, England.
43. Snedecor, G. W. & Cochran, W. G. (1967) Statistical Methods. Iowa State University Press, Ames, IA.
44. Steinrucken, H. C. & Amrhein, N. (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl shikimic acid-3-phosphate synthase. Biochem. Biophys. Res. Comm. 94: 1207-1212.
45. Steinrucken, H. C. & Amrhein, N. (1984) 5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae: inhibition by glyphosate [N-(phosphonomethyl)glycine]. Eur. J. Biochem. 143: 351-357.
46. Taylor, S. L., Lemanske, R. F., Jr., Bush, R. K. & Busse, W. W. (1987) Food allergens: structure and immunologie properties. Ann. Allergy 59: 93-99.
47. Taylor, S. L., Nordlee, J. A. & Bush, R. K. (1992) Food allergies. In: Food Safety Assessment, ACS Symposium Series 484 (Finley, J. W., Robinson, S. F. & Armstrong, D. J., ed.), pp. 316-329. American Chemical Society, Washington, DC.
48. Technical Assessment Systems (1993) Exposure 1, Chronic Dietary Exposure Analysis Program (1987-88 or 1977-78 USDA surveys). Technical Assessment Systems, Inc., Washington, DC.
49. U.S. Food and Drug Administration (1992) Foods derived from new plant varieties. Federal Register 57 (104): 22984-23005.
50. USP (1990) The United States Pharmacopeia, Vol. XXII, NFXVII. United States Pharmacopeial Convention, Inc., Rockville, MD.
51. University of Wisconsin Genetics Computer Group (1991) Genetics Computer Group, Program Manual for the GCG Package, Version 7, April 1991. University of Wisconsin Genetics Computer Group, Madison, WI.
52. Wilbur, W. J. & Lipman, D. J. (1983) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. U.S.A. 80: 726-730.
53. Zikakis, J. P., Rzucidlo, S. J. & Biasotto, N. O. (1977] Persistance of bovine milk xanthine oxidase activity after gastric digestion in vivo and in vitro. J. Dairy Sci. 60: 533-541.