Insect cells infected with a recombinant baculovirus express both O- and N-glycosylated forms of the rat glycoprotein hormone α-subunit

Journal of Molecular Endocrinology

Volumn 16, Issue 2, 1996, Pages 141-149

  Delahaye, R ^a   Berreur, P ^a   Salesse, R ^b   Counis, R ^a  

^a SORBONNE UNIVERSITÉ   (France)

^b CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ENDOGENOUS COMPOUND; GLYCOPROTEIN;

ALPHA CHAIN; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; VIRUS INFECTION;

EID: 0029977733     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0160141     Document Type: Article

References (54)

1. Baenziger JU 1990 The asparagine-linked oligosaccharides of the glycoprotein hormones. In Glycoprotein Hormones: Structure, Synthesis and Biologic Function, pp 1-10. Eds WW Chin & I Boime. Norwell: Serono Symposia USA.
2. Bedows E, Huth JR & Ruddon RW 1991 Kinetics and assembly of the human chorionic gonadotropin β subunit in transfected Chinese hamster ovary cells. Journal of Biological Chemistry 267 8880-8886.
3. Begeot M, Hemming FJ, Dubois PM, Combarnous Y, Dubois MP & Aubert ML 1984 Induction of pituitary lactotrope differentiation by luteinizing hormone α-subunit. Science 226 566-568.
4. Bidard JM, Troalen F, Salesse R, Bousfield GR, Bohuon CJ & Bellet DH 1987 Topographic antigenic determinants recognized by monoclonal antibodies on human choriogonadotropin β-subunit. Journal of Biological Chemistry 262 8551-8556.
5. Bielinska M & Boime I 1979 Glycosylation of human chorionic gonadotropin in mRNA-dependent cell-free extracts: posttranslational processing of an asparagine-linked mannose-rich oligosaccharide. Proceedings of the National Academy of Sciences of the USA 76 1208-1212.
6. Birken S & Canfield RE 1977 Isolation and amino acid sequence of COOH-terminal fragments from the β subunit of human choriogonadotropin. Journal of Biological Chemistry 252 5386-5392.
7. Blithe DL & Iles RK 1995 The role of glycosylation in regulating the glycoprotein hormone free α-subunit and free β-subunit combination in the extraembryonic coelomic fluid of early pregnancy. Endocrinology 136 903-910.
8. Blithe DL, Richards RG & Skarulis MC 1991 Free α-molecules from pregnancy stimulate secretion of prolactin from human decidual cells: a novel function for free α in pregnancy. Endocrinology 129 2257-2259.
9. Boothby M, Kukowska J & Boime I 1983 Imbalanced synthesis of human choriogonadotropin α- and β-subunits reflects the steady-state levels of the corresponding mRNAs. Journal of Biological Chemistry 258 9250-9253.
10. Burnside J, Buckland PR & Chin WW 1988 Isolation and characterization of the gene encoding the α-subunit of the rat pituitary glycoprotein hormones. Gene 70 67-74.
11. Campbell RK, Erfle H, Barnett RW & Moyle WR 1992 Assembly and expression of a synthetic gene encoding the bovine glycoprotein hormone α-subunit. Molecular and Cellular Endocrinology 83 195-200.
12. Christophe S, Robert P, Maugain S, Bellet D, Koman A & Bidard JM 1993 Expression of the human follicle-stimulating hormone receptor in the baculovirus system. Biochemical and Biophysical Research Communications 196 402-408.
13. Combarnous Y 1992 Molecular basis of the specificity of binding of glycoprotein hormones to their receptors. Endocrine Reviews 13 670-691.
14. Counis R & Jutisz M 1991 Regulation of pituitary gonadotropin gene expression. Outline of intracellular signaling pathways. Trends in Endocrinology and Metabolism 2 181-187.
15. Eckhardt AE, Timpte CS, Abernethy JL, Toumadje A, Johnson WC & Hill RL 1987 Structural properties of porcine submaxillary gland apomucin. Journal of Biological Chemistry 262 11339-11344.
16. Feng W, Huth JR, Norton SE & Ruddon RW 1995 Asparagine-linked oligosaccharides facilitate human chorionic gonadotropin β-subunit folding but not assembly of prefolded β with α Endocrinology 136 52-61.
17. Franchimont P & Pasteels JL 1972 Sécrétion indépendante des hormones gonadotropes et de leurs sous-unités. Comptes Rendus de l'Académie des Sciences de Paris Série D 275 1799-1802.
18. Gharib SD, Wierman ME, Shupnik MA & Chin WW 1990 Molecular biology of the pituitary gonadotropins. Endocrine Reviews 11 177-199.
19. Gooley AA & Williams KL 1994 Towards characterizing O-glycans: the relative merits of in vivo and in vitro approaches in seeking peptide motifs specifying O-glycosylation sites. Glycobiology 4 413-417.
20. Grotjan HEJ, Leveque NW, Berkowitch AS & Keel BA 1984 Quantitation of LH subunits released by rat anterior pituitary cells in primary culture. Molecular and Cellular Endocrinology 35 121-129.
21. Hagen C & McNeilly AS 1975 Identification of human luteinizing hormone, follicle-stimulating hormone, luteinizing hormone β-subunit and gonadotrophin α-subunit in foetal and adult pituitary glands. Journal of Endocrinology 67 49-57.
22. Helenius A 1994 How N-linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum. Molecular Biology of the Cell 5 253-265.
23. Hoshina H & Boime I 1982 Combination of rat lutropin subunits occurs early in the secretory pathway. Proceedings of the National Academy of Sciences of the USA 79 7649-7653.
24. Huang CJ, Huang FL, Chang GD, Chang YS, Lo CF, Fraser MJ & Lo TB 1991 Expression of two forms of carp gonadotropin α-subunit in insect cells by recombinant baculovirus. Proceedings of the National Academy of Sciences of the USA 88 7486-7490.
25. Jha PK, Pal R, Nakhai B, Sridhar P & Hasnain SE 1992 Simultaneous synthesis of enzymatically active luciferase and biologically active beta-subunit of human chorionic gonadotropin in caterpillars infected with a recombinant baculovirus. FEBS Letters 310 148-152.
26. Kaetzel DM, Virgin JB, Clay CM & Nilson JH 1989 Disruption of N-linked glycosylation of bovine luteinizing hormone β-subunit by site-directed mutagenesis dramatically increases its intracellular stability but does not affect biological activity of the secreted heterodimer. Molecular Endocrinology 3 1765-1774.
27. Keutmann HT & Williams RM 1977 Human chorionic gonadotropin: amino acid sequence of the hormone-specific COOH-terminal region. Journal of Biological Chemistry 252 5393-5397.
28. Laburthe MC, Dolais JR & Rosselin GE 1973 Evidence for circulating α-subunits of pituitary gonadotropins in plasma. Journal of Clinical Endocrinology and Metabolism 37 156-159.
29. Laemmli UK 1970 Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227 680-688.
30. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ & Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369 455-461.
31. Maruniak JE & Summers MD 1981 Autographs californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. Virology 109 25-34.
32. Matzuk MM & Boime I 1988 The role of the asparagine-linked oligosaccharides of the a subunit in the secretion and assembly of human chorionic gonadotropin. Journal of Cell Biology 106 1049-1059
33. Matzuk MM, Keene JL & Boime I 1989 Site specificity of the chorionic gonadotropin N-linked oligosaccharides in signal transduction. Journal of Biological Chemistry 264 2409-2414.
34. Misrahi M, Ghinea N, Sar S, Saunier B, Jolivet A, Loosfek H, Cerutti M, Devauchelle G & Milgrom E 1994 Processing of the precursors of the human thyroid-stimulating hormone-receptor in various eukaryotic cells (human thyrocytes, transfected cells and baculovirus-infected cells). European Journal of Biochemistry 222 711-719.
35. Nakhai B, Pal R, Sridhar P, Talvsar GP & Hasnain SE 1991 The α-subunit of human chorionic gonadotropin hormone synthesized in insect cells using a baculovirus vector is biologically active. FEBS Letters 283 104-108.
36. O'Connell B, Tabak LA & Ramasubbu N 1991 The influence of flanking sequences on O-glycosylation. Biochemical and Biophysical Research Communications 180 1024-1030.
37. O'Reilly DR, Miller LK & Luckow VA 1992 Baculovirus Expression Vectors - A Laboratory Manual. New York: WH Freeman & Company.
38. Pajot-Augy E, Couture L, Bozon V, Remy J-J, Biache G, Severini M, Huet J-C, Pernollet J-C & Salesse R 1995 High-level expression of recombinant porcine LH receptor in baculovirus-infected insect cells or caterpillars. Journal of Molecular Endocrinology 14 51-66.
39. Parsons TF & Pierce JG 1984 Free α-like material from bovine pituitaries. Removal of its O-linked oligosacharide permits combination with lutropin-β. Journal of Biological Chemistry 259 2662-2666.
40. Pierce JG & Parsons TF 1981 Glycoprotein hormones : structure and function. Annual Review of Biochemistry 50 465-495.
41. Prentice LG & Ryan RJ 1975 LH and its subunits in human pituitary, serum and urine. Journal of Clinical Endocrinology and Metabolism 40 303-312.
42. Rafelson ME Jr, Clauser H & Legault-Demare J 1961 Removal of sialic acid from serum gonadotropin by acidic and enzymic hydrolysis. Biochimica et Biophysica Acta 47 406-407.
43. Salesse R, Bidard JM, Troalen F, Bellet D & Garnier J 1990 Peptide mapping of intersubunit and receptor interactions of human choriogonadotropin. Molecular and Cellular Endocrinology 68 113-119.
44. Sambrook J, Fritsch EF & Maniatis T 1989 Molecular Cloning - A Laboratory Manual, edn 2. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
45. Shogren R, Gerken TA & Jentoft N 1989 Role of glycosylation on the conformation and chain dimensions of O-linked glycoproteins: light-scattering studies of ovine submaxillary mucin. Biochemistry 28 5525-5536.
46. Smith PL & Baenziger JU 1988 A pituitary N-acetylgalactosamme transferase that specifically recognizes glycoprotein hormones. Science 242 930-932.
47. Sridhar P & Hasnain SE 1993 Differential secretion and glycosylation of recombinant human chorionic gonadotropin (beta-hCG) synthesized using different promoters in the baculovirus expression vector system. Gene 131 261-264.
48. Starzec A, Counis R & Jutisz M 1986 Gonadotropin-releasing hormone stimulates the synthesis of the polypeptide chains of luteinizing hormone. Endocrinology 119 561-565.
49. Starzec A, Counis R & Jutisz M 1988 Several intermediate forms in the processing of rat lutropin subunits as shown by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Journal of Chromatography 440 353-360.
50. Stewart EA, Rein MS, Friedman AJ, Zuchowski L & Nowak RA 1994 Glycoprotein hormones and their common α-subunit stimulate prolactin production by explant cultures of human leiomyoma and myometrum. American Journal of Obstetrics and Gynecology 170 677-683.
51. Summers MD & Smith GE 1987 A manual of methods for baculovirus vectors and insect cell culture procedure. Texas Agricultural Experiment Station Bulletin 1555 3-55.
52. Vlak JM, Schlaeger EJ & Bernard AR 1992 Workshop on Baculovirus and Recombinant Protein Production Processes. Basel: Roche.
53. Wilson IBH, Gavel Y & von Heijne G 1991 Amino acid distributions around O-linked glycosylation sites. Biochemical Journal 275 529-534.
54. Wu H, Lustbader JW, Liu Y, Canfield RE & Hendrickson WA 1994 Structure of human chorionic gonadotropin at 2.6 Å resolution from MAD analysis of the selenomethionyl protein. Structure 2 545-558.