Comparative immunogold analysis of tubulin isoforms in the mouse sperm flagellum: Unique distribution of glutamylated tubulin

Molecular Reproduction and Development

Volumn 43, Issue 3, 1996, Pages 358-365

  Fouquet, Jean Pierre ^a   Prigent, Yann ^a   Kann, Marie Louise ^a  

^a UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

Axoneme; Cytoskeleton; Microtubule; Spermatozoa

Indexed keywords

ALPHA TUBULIN; BETA TUBULIN; TUBULIN;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; AXONEME; IMMUNOGOLD STAINING; MALE; MICROTUBULE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN POLYMORPHISM; SPERMATOZOON TAIL;

ANIMALS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; MALE; MICE; SPERM TAIL; TUBULIN;

ANIMALIA; MAMMALIA;

EID: 0029976429     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199603)43:3<358::AID-MRD10>3.0.CO;2-Y     Document Type: Article

References (23)

1. Andreu JM, de Pereda JM (1993): Site-directed antibodies to tubulin. Cell Motil Cytoskel 26:1-6.
2. Avila J (1992): Microtubule functions. Life Sci 50:327-334.
3. Boucher D, Larcher JC, Gros F, Denoulet P (1994): Polyglutamylation of tubulin as a progressive regulator of in vitro interactions between the microtubule-associated protein tau and tubulin. Biochemistry 33:12471-12477.
4. Brown A, Li Y, Slaughter T, Black MM (1993): Composite microtubules of the axon: Quantitative analysis of tyrosinated and acetylated tubulin along individual axonal microtubules. J Cell Sci 104: 339-352.
5. Bulinski JC, Gundersen GG (1991): Stabilization and posttranslational modification of mocrotubules during cellular morphogenesis. Bioessays 13:285-293.
6. Cassimeris L (1993): Regulation of microtubule dynamic instability. Cell Motil Cytoskel 26:275-281.
7. Chapin SJ, Bulinski JC (1992): Microtubule stabilization by assembly-promoting microtubule-associated proteins: A repeat performance. Cell Motil Cytoskel 23:236-243.
8. Eddy EM, O'Brien DA (1994): The spermatozoon. In E Knobil, JD Neil (eds): "The Physiology of Reproduction," Vol. I. New York: Raven Press, pp 29-77.
9. Fouquet JP, Kann ML (1994): The cytoskeleton of mammalian spermatozoa. Biol Cell 81:89-93.
10. Fouquet JP, Eddé B, Kann ML, Wolff A, Desbruyères E, Denoulet P (1994): Differential distribution of glutamylated tubulin during spermatogenesis in mammalian testis. Cell Motil Cytoskel 27:49-58.
11. Gagnon C, White D, Cosson J, Huitorel P, Eddé B, Paturlé L, Multigner L, Job D (1995): New antitubulin antibodies prevent axonemal motility in flagella. J Cell Biochem Suppl 19B: 112.
12. Geuens G, Gundersen GG, Nuydens R, Comelissen F, Bulinski JC, Debradander M (1986): Ultrastructural colocalization of tyrosinated and detyrosinated α-tubulin in interphase and mitotic cells. J Cell Biol 103:1883-1893.
13. Hecht NB, Distel RJ, Yelick PC, Tanhauser SM, Driscoll CE, Goldberg E, Tung KSK (1988): Localization of a highly divergent mammalian testicular α tubulin that is not detectable in brain. Mol Cell Biol 8:996-1000.
14. Hermo L, Oko R, Hecht NB (1991): Differential posttranslational modifications of microtubules in cells of the seminiferous epithelium of the rat: A light and electron microscope immunocytochemical study. Anat Rec 229:31-50.
15. Kann ML, Prigent Y, Fouquet JP (1995): Differential distribution of glutamylated tubulin in the flagellum of mouse spermatozoa. Tissue Cell 27:323-329.
16. 2+: Evidence for a differential response of certain microtubule pairs within the bovine sperm axoneme. Cell Motil Cytoskel 26:66-76.
17. Lewis SA, Cowan NJ (1988). Complex regulation and functional versatility of mammalian α- and β-tubulin isotypes during the differentiation of testis and muscle cells. J Cell Biol 106:2023-2033.
18. Mitchell DR (1994): Cell and molecular biology of flagellar dyneins. Int Rev Cytol 155:141-180.
19. Piperno G, Fuller MT (1985): Monoclonal antibodies specific for an acetylated form of α-tubulin recognize the antigen in cilia and flagella from a variety of organisms. J Cell Biol 101:2085-2094.
20. Simerly CR, Hecht NB. Goldberg E, Schatten G (1993): Tracing the incorporation of the sperm tail in the mouse zygote and early embryo using an anti-testicular α-tubulin antibody. Dev Biol 158:536-548.
21. Smith EF, Sale WS (1992): Regulation of dynein-driven microtubule sliding by the radial spokes in flagella. Science 257:1557-1559.
22. Wolff A, de Néchaud B, Chillet D, Mazarguil H, Desbruyères E, Audebert S, Eddé B, Gros F, Denoulet P (1992): Distribution of glutamylated α and β-tubulin in mouse tissues using a specific monoclonal antibody, GT355, Eur J Cell Biol 59:425-432.
23. Wolff A, Houdayer M, Chillet D, de Néchaud B, Denoulet P (1994): Structure of the polyglutamyl chain of tubulin: Occurrence of alpha and gamma linkages between glutamyl units revealed by monoreactive polyclonal antibodies Biol Cell 81:11-16.