Localization of ribosomal RNA and Pbs21-mRNA in the sexual stages of Plasmodium berghei using electron microscope in situ hybridization

European Journal of Cell Biology

Volumn 71, Issue 3, 1996, Pages 270-276

  Shaw, Michael K ^a   Thompson, Joanne ^a   Sinden, Robert E ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Electron microscopy; In situ hybridization; Pbs21 messenger RNA; Plasmodium berghei; Ribosomal RNA; Sexual stages

Indexed keywords

MESSENGER RNA; PROTEIN; RIBOSOME RNA;

ANIMAL TISSUE; ARTICLE; CELL NUCLEUS INCLUSION BODY; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; CYTOPLASM; ELECTRON MICROSCOPY; GAMETOCYTE; IN SITU HYBRIDIZATION; NONHUMAN; PLASMODIUM BERGHEI; PRIORITY JOURNAL; RIBOSOME; RNA ANALYSIS; RNA PROBE; SEXUAL DEVELOPMENT; ZYGOTE;

ANIMALIA; PLASMODIUM BERGHEI;

EID: 0029973964     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Aikawa, M., C. G. Huff, H. Sprinz: Comparative fine structure study of the gametocytes of avian, reptilian and mammalian malaria. J. Ultrastruct. Res. 26, 316-331 (1969).
2. Alano, P., R. Carter: Sexual differentiation in malaria parasites. Annu. Rev. Microbiol. 44, 429-449 (1990).
3. Bienz, K., D. Egger: Immunocytochemistry and in situ hybridization in the electron microscope: combined application in the study of virus-infected cells. Histochemistry 103, 325-338 (1995).
4. Dame, T. B., T. F. McCutchan: Identification of the 5S and 5.8S ribosomal RNA molecules and their genes in Plasmodium berghei. Mol. Biochem. Parasitol. 11, 301-307 (1984).
5. Hozak, P., P. R. Cook: Replication factories. Trends Cell Biol. 4, 48-52 (1994).
6. Hozak, P., P. R. Cook, C. Schofer, W. Mosgöller, F. Wachtler: Sites of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J. Cell Sci. 107, 639-648 (1994).
7. Hozak, P., D. A. Jackson, P. R. Cook: Replication factories and nuclear bodies: the ultrastructural characterization of replication sites during the cell cycle. J. Cell Sci. 107, 2191-2202 (1994).
8. Hughes, T. A., A. Pombo, J. McManos, P. Hozak, D. A. Jackson, P. R. Cook: On the structure of replication and transcription factories. J. Cell Sci. Supp. 19, 59-65 (1995).
9. McCutchan, T. F., G. A. McConkey, M. J. Rogers, A. P. Waters: RNAs of Plasmodium spp. Parasitol. Today 11, 134-138.
10. McFadden, G. I.: In situ hybridization techniques: Molecular cytology goes ultrastructural. In J. L. Hall, C. Hawes (eds.): Electron Microscopy of Plant Cells. pp. 219-255. Academic Press Ltd. London 1991.
11. Morel, G.: Hybridization Techniques for Electron Microscopy. CRC Press Inc. Boca Raton/USA 1993.
12. Paton, M. G., G. C. Baker, H. Matsuoka, J. Ramesar, C. J. Janse, A. P. Waters, R. E. Sinden: Structure and expression of a conserved and post-transcriptionally regulated gene encoding a surface protein of the sexual stages from the malaria parasite Plasmodium berghei. Mol. Biochem. Parasitol. 59, 263-276 (1993).
13. Raska, I., M. Dundr, K. Koberna, I. Melcak, M.-C. Risueño, I. Torok: Does the synthesis of ribosomal RNA take place within nucleolar fibrillar centers or dense fibrillar components? A critical appraisal. J. Structural Biol. 114, 1-22 (1995).
14. St. Johnston, D.: The intracellular localization of messenger RNAs. Cell 81, 161-170 (1995).
15. Sinden, R. E.: Cell biology. In: R. Killick-Kendrick, W. Peters (eds.): Rodent Malaria. pp. 85-168. Academic Press. London 1978.
16. Sinden, R. E.: Sexual development of malarial parasites. Adv. Parasitol. 22, 153-216 (1983).
17. Sinden, R. E., R. H. Hartley, L. Winger: Plasmodium ookinetes in vitro: an ultrastructural study including a description of meiotic division. Parasitology 91, 227-244 (1985).
18. Sinden, R. E., L. Winger, R. H. Carter, R. H. Hartley, N. Tirawanchai, C. S. Davies, J. Moore, J. F. Sluiters: Ookinete antigens of Plasmodium berghei: a light and electron microscope immunogold study of expression of the 21 kDa determinant recognized by a transmission-blocking antibody. Proc. Roy. Soc. London B. 230, 443-458 (1987).
19. Thompson, J., R. E. Sinden: In situ detection of Pbs21 mRNA during sexual development of Plasmodium berghei. Mol. Biochem. Parasitol. 68, 189-196 (1994).
20. Vervenne, R. A. W., R. W. Dirks, J. Ramesar, A. P. Waters, C. J. Janse: Differential expression in blood stages of the gene coding for the 21-kilodalton surface protein of ookinetes of Plasmodium berghei as detected by RNA in situ hybridization. Mol. Biochem. Parasitol. 68, 259-266 (1994).
21. Waters, A. P., R. M. L. Spaendonk, J. Ramesar, J. Thompson, R. A. W. Vervenne, R. W. Dirks, C. J. Janse: Species specific regulation and switching of expression between stage specific ribosomal RNA genes in Plasmodium berghei. J. Biol. Chem., in press (1996).
22. Wilhelm, J. E., R. D. Vale: RNA on the move: the mRNA localization pathway. J. Cell Biol. 123, 269-274 (1993).
23. Winger, L. A., N. Tirawanchai, J. Nicholas, H. E. Carter, J. E. Smith, R. E. Sinden: Ookinete antigens of Plasmodium berghei: Appearance on the zygote surface of an Mr 21Kd determinant identified by transmission-blocking monoclonal antibodies. Parasite Immunol. 10, 193-207 (1988).