Subcloning of a DNA fragment encoding a single cohesin domain of the Clostridium thermocellum cellulosome-integrating protein CipA: Purification, crystallization, and preliminary diffraction analysis of the encoded polypeptide

Protein Science

Volumn 5, Issue 6, 1996, Pages 1192-1194

  Béguin, Pierre ^a   Raynaud, Odette ^a   Chaveroche, Marie Kim ^a   Dridi, Abel ^a   Alzari, Pedro M ^b  

^a CNRS   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

Clostridium thermocellum; Cohesin domain; Crystallization; Scaffolding protein; X ray crystallography

Indexed keywords

CLOSTRIDIUM THERMOCELLUM; ESCHERICHIA COLI;

CELLULOSE; DNA FRAGMENT; MACROGOL 8000; PAPAIN; POLYPEPTIDE; PROTEIN;

ARTICLE; CLOSTRIDIUM THERMOCELLUM; CRYSTALLIZATION; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; X RAY CRYSTALLOGRAPHY; X RAY DIFFRACTION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; CLONING, MOLECULAR; CLOSTRIDIUM; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DNA, BACTERIAL; DNA, RECOMBINANT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; MEMBRANE PROTEINS; PLASMIDS; RECOMBINANT PROTEINS;

EID: 0029973832     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050623     Document Type: Article

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