Immunoreactivity of specific epitopes of PrP(Sc) is enhanced by pretreatment in a hydrated autoclave

Clinical and Diagnostic Laboratory Immunology

Volumn 3, Issue 4, 1996, Pages 470-471

  Yokoyama, Takashi ^a   Momotani, Eiichi ^a   Kimura, Kumiko ^a   Yuasa, Noboru ^a  

^a NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; AUTOCLAVE; EPITOPE MAPPING; HYDRATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEINASE INHIBITION; RNA TRANSLATION;

EID: 0029973206     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.3.4.470-471.1996     Document Type: Article

References (13)

1. Bolton, D. C., M. P. McKinley, and S. B. Prusiner. 1982. Identification of a protein that purifies with the scrapie prion. Science 218:1309-1311.
2. Caughey, B., R. E. Race, D. Ernst, M. J. Buchmeier, and B. Chesebro. 1989. Prion protein biosynthesis in scrapie-infected and uninfected neuroblastoma cells. J. Virol. 63:175-181.
3. Gajdusek, D. C. 1977. Unconventional viruses and the origin and disappearance of Kuru. Science 197:943-960.
4. A. Haritani, M., Y. I. Spencer, and G. A. H. Wells. 1994. Hydratcd autoclave pretreatment enhancement of prion protein immunoreactivity in formalin-fixed bovine spongiform encephalopathy-affected brain. Acta Neuropathol. 87:86-90.
5. Ikegami, Y., M. Ito, H. Isomura, E. Momotani, K. Sasaki, V. Muramatsu, N. Ishiguro, and M. Shinagawa. 1991. Preclinical and clinical diagnosis of scrapie by detection of PrP protein in tissues of sheep. Vet. Rec. 128:271-275.
6. Meyer, R. K., M. P. McKinley, K. A. Bowman, M. B. Braunfeld, R. A. Barry, and S. B. Prusiner. 1986. Separation and properties of cellular and scrapie prion proteins. Proc. Natl. Acad. Sci. USA 83:2310-2314.
7. Oesch, B., D. Westaway, M. Wälchli, M. P. McKinley, S. B. H. Kent, R. Aebersold, R. A. Barry, P. Tempst, D. B. Teplow, L. E. Hood, S. B. Prusiner, and C. Weissmann. 1985. A cellular gene encodes scrapie PrP 27-30 protein. Cell 40:735-746.
8. Prusiner, S. B. 1991. Molecular biology of prion diseases. Science 252:1515-1522.
9. Race, R., D. Ernst, A. Jenny, W. Taylor, D. Sutton, and B. Caughey. 1992. Diagnostic implications of detection of proteinase K-resistant protein in spleen, lymph nodes, and brain of sheep. Am. J. Vet. Res. 53:883-889.
10. Serban, D., A. Taraboulos, S. J. DeArmond, and S. B. Prusiner. 1990. Rapid detection of Creutzfeldt-Jakob disease and scrapie prion proteins. Neurology 40:110-117.
11. Shinagawa, M., K. Takahashi, S. Sasaki, S. Doi, H. Goto, and G. Sato. 1985. Characterization of scrapie agent isolated from sheep in Japan. Microbiol. Immunol. 29:543-551.
12. Yokoyama, T., S. Itohara, and N. Yuasa. 1996. Detection of species specific epitopes of mouse and hamster prion protein (PrPs) by anti-peptide anti-bodies. Arch. Virol. 141:763-769.
13. Yokoyama, T., K. Kimura, Y. Tagawa, and N. Yuasa. 1995. Preparation and characterization of antibodies against mouse prion protein (PrP) peptides. Clin. Diag. Lab. Immunol. 2:172-176.