Circadian variation of DNA replication in hamster cheek pouch epithelium analysed by tritiated thymidine labelling, flow sorting and autoradiography: No resting S phase cells in the normal epithelium

Cell Proliferation

Volumn 29, Issue 2, 1996, Pages 61-71

  Larsen, J K ^a   Moller U ^a   Christensen, I J ^a   Christiansen, J ^a  

^a FINSEN LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

THYMIDINE; TRITIUM;

ANIMAL TISSUE; ARTICLE; AUTORADIOGRAPHY; CELL CYCLE S PHASE; CELL PROLIFERATION; CELL SELECTION; CHEEK MUCOSA; CIRCADIAN RHYTHM; DNA REPLICATION; HAMSTER; MALE; NONHUMAN;

EID: 0029972828     PISSN: 09607722     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2184.1996.tb00095.x     Document Type: Article

References (22)

1. CHRISTENSEN I, HARTMANN NR, KEIDING N, LARSEN JK, NOER H, VINDELØV L. (1978) Statistical analysis of DNA distributions from cell populations with partial synchrony. In: Lutz D, ed. Pulse-Cytophotometry III. Ghent: European Press, 71.
2. CLAUSEN OP, ELGJO K, KIRKHUS B, PEDERSEN S, BOLUND L. (1983) DNA synthesis in mouse epidermis: S phase cells that remain unlabeled after pulse labeling with DNA precursors progress slowly through S. J. Invest. Dermatol. 81, 545.
3. CLAUSEN OP, KIRKHUS B, ELGJO K, PEDERSEN S, BOLUND L. (1985) DNA synthesis rate changes during the S phase in mouse epidermis. Cell Tissue Kinet. 18, 445.
4. CLAUSEN OP, KIRKHUS B, THORUD E, SCHJØLBERG A, MOEN E, CROMARTY A. (1986) Evidence of mouse epidermal subpopulations with different cell cycle times. J. Invest. Dermatol. 86, 266.
5. HUME WJ, POTTEN CS. (1982) A long-lived thymidine pool in epithelial stem cells. Cell Tissue Kinet. 15, 49.
6. JENSEN PK, PEDERSEN S, BOLUND L. (1985) Basal-cell subpopulations and cell-cycle kinetics in human epidermal explant cultures. Cell Tissue Kinet. 18, 201.
7. KATO K, RADBRUCH A. (1993) Isolation and characterization of CD34+ hematopoietic stem cells from human peripheral blood by high-gradient magnetic cell sorting. Cytometry 14, 384.
8. KEIDING N, HARTMANN NR, MØLLER U. (1984) Diurnal Variation in influx and transition intensities in the S phase of hamster cheek pouch epithelium cells. In: Edmunds LN, Jr, ed. Cell Cycle Clocks. New York, USA: Marcel Dekker, 135.
9. KIRKHUS B, CLAUSEN OP. (1990) Cell kinetics in mouse epidermis studied by bivariate DNA/bromodeoxyuridine and DNA/keratin flow cytometry. Cytometry 11, 253.
10. KIRKHUS B, CLAUSEN OP. (1992a) Circadian variations in cell cycle phase progression of mouse epidermal cells measured directly by bivariate BrdUrd/DNA flow cytometry. Epithelial Cell. Biol. 1, 32.
11. KIRKHUS B, GLASØ M, CLAUSEN OP. (1992b) Multivariate flow cytometry of epidermal regeneration provoked by a skin irritant and a tumor promoter. Cytometry 13, 267.
12. LARSEN JK, FRENTZ G, MØLLER U, CHRISTENSEN IJ. (1985) A method for flow cytometric cell cycle anaysis of normal and psoriatic human epidermis based on a detergent/citric acid technique for suspension of nuclei. Virchows Arch. B Cell Pathol. 48, 247.
13. LARSEN JK, MUNCH-PETERSEN B, CHRISTIANSEN J, JØRGENSEN K. (1986) Flow cytometric discrimination of mitotic cells: resolution of M, as well as G1, S, and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde. Cytometry 7, 54.
14. LARSEN JK. (1994) Principles, capacity and limitations of immunofluorescent flow-sorting of rare cell species. In: Wunder E, Sovalat H, Henon P, et al., eds. Hematopoietic stem cells: The Mulhouse Manual. Dayton, Ohio, USA: AlphaMed Press, 133.
15. MØLLER U. (1984) Diurnal rhythmicity used in experimental cancer research. In: Edmunds LN, Jr., ed. Cell Cycle Clocks. New York, USA: Marcel Dekker, 501.
16. MØLLER U, KEIDING N. (1982a) Circadian variations in influx and efflux of the S phase in a partially synchronized cell system. Double-labelling with [3H]thymidine in the epithelium of the hamster cheek pouch. Cell Tissue Kinet. 15, 341.
17. MØLLER U, KEIDING N, ENGEL F. (1982b) Subclassification of cells in S phase in a partially synchronized cell system. Cell Tissue Kinet. 15, 157.
18. MØLLER U, KEIDING N, HOUGAARD P. (1985) Circadian variation in mitotic influx in a keratinized epithelium. The stathmokinetic technique used in vivo on the hamster cheek pouch epithelium and analyzed by periodic regression analysis. Virchows Arch. B Cell Pathol. 48, 119.
19. MØLLER U, KEIDING N, LARSEN JK. (1984a) Effect of methotrexate on cells in a keratinized epithelium with an active thymidine salvage pathway assessed by autoradiography. Cell Tissue Kinet. 17, 497.
20. MØLLER U, LARSEN JK. (1978) The circadian variations in the epithelial growth of the hamster cheek pouch: quantitative analysis of DNA distributions. Cell Tissue Kinet. 11, 405.
21. MØLLER U, LARSEN JK. (1979) DNA flow cytometry of isolated keratinized epithelia: a methodological study based on ultrasonic tissue disaggregation. Cell Tissue Kinet. 12, 203.
22. MØLLER U, LARSEN JK, KEIDING N, CHRISTENSEN IJ. (1984b) Circadian-stage dependence of methotrexate in a keratinized epithelium. An in-vivo study using flow cytometry on the hamster cheek pouch epithelium. Cell Tissue Kinet. 17, 483.