Interlobe communication in multiple calcium-binding site mutants of Drosophila calmodulin

Protein Science

Volumn 5, Issue 3, 1996, Pages 468-477

  Mukherjea, Poushali ^a,b   Maune, John F ^a   Beckingham, Kathy ^a  

^a RICE UNIVERSITY   (United States)

^b RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

calcium binding proteins; circular dichroism; skeletal muscle myosin light chain kinase; UV difference spectroscopy

Indexed keywords

CALCIUM BINDING PROTEIN; CALMODULIN; MYOSIN LIGHT CHAIN KINASE;

ARTICLE; BINDING SITE; CALCIUM BINDING; CELL COMMUNICATION; CIRCULAR DICHROISM; DELETION MUTANT; DROSOPHILA; ENZYME ACTIVATION; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; SKELETAL MUSCLE;

EID: 0029972702     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050308     Document Type: Article

References (30)

1. Babu YS, Bugg CE, Cook WJ. 1988. Structure of calmodulin refined at 2.2 Å resolution. J Mol Biol 204:191-204.
2. 15N relaxation using inverse detected two-dimensional NMR spectroscopy: The central helix is flexible. Biochemistry 31:5269-5278.
3. Cook WJ, Walter LJ, Walter MR. 1994. Drug binding to calmodulin: Crystal structure of a calmodulin-trifluoperazine complex. Biochemistry 33:15259-15265.
4. Donovan JW. 1973. Ultraviolet difference spectroscopy - New techniques and applications. Biochemistry 33:15259-15265.
5. Forsén S, Vogel HJ, Drakenberg T. 1986. Biophysical studies of calmodulin. In: Cheung WY, ed. Calcium and cell function, vol VI. New York: Academic Press, pp 113-157.
6. 2+ binding sites. J Biol Chem 268:20096-20104.
7. Heidorn DB, Trewhella J. 1988. Comparison of the crystal and solution structures of calmodulin and troponin C. Biochemistry 27:909-915.
8. 13C resonance assignments of calmodulin in solution by heteronuclear multidimensional NMR spectroscopy. Biochemistry 30:9216-9228.
9. Kretsinger RH, Nockolds CE. 1973. Carp muscle calcium-binding protein. J Biol Chem 248:3313-3326.
10. Kuboniwa H, Tjandra N, Grzesiek S, Ren H, Klee CB, Bax A. 1995. Solution structure of calcium-free calmodulin. Nature Struct Biol 2:768-776.
11. Linse S, Helmersson A, Forsen S. 1991. Calcium binding to calmodulin and its globular domains. J Biol Chem 266:8050-8054.
12. 2+ on the secondary and tertiary structure of bovine testis calmodulin. A circular-dichroism study. Biochem J 238:485-490.
13. Martin SR, Maune JF, Beckingham K, Bayley PM. 1992. Stopped-flow studies of calcium dissociation from caicium-binding-site mutants of Drosophila melanogaster Eur J Biochem 205:1107-1114.
14. 2+ binding site mutants of calmodulin. Biochemistry 31:7779-7186.
15. 2+ binding sues of calmodulin. J Biol Chem 267:5286-5295.
16. Mukherjea P. 1995. Studies of the roles of the individual calcium binding sites of Drosophila calmodulin in conformational change and target interaction [thesis]. Houston, Texas: Rice University.
17. Mukherjea P, Beckingham K. 1993. Calcium binding site mutants of calmodulin adopt abnormal conformations in complexes with model target peptides. Biochem Mol Biol Int 29:555-563.
18. Newton DL, Krinks MH, Kaufman JB, Shiloach J, Klee CB. 1988. Large scale preparation of calmodulin. Preparative Biochemistry 18:241-259.
19. Richman PG, Klee CB. 1978. Conformation-dependent nitration of the protein activator of cyclic adenosine 3′,5′-monophosphate phosphodiesterase. Biochemistry 17:928-935.
20. 2+ of tyrosyl residue 138 of calmodulin. J Biol Chem 254:5372-5376.
21. Seamon KB. 1980. Calcium- and magnesium-dependent conformational states of calmodulin as determined by nuclear magnetic resonance. Biochemistry 19:207-215.
22. Seaton BA, Head JF, Engelman DM, Richards FM. 1985. Calcium-induced increase in the radius of gyration and maximum dimension of calmodulin measured by small-angle X-ray scattering. Biochemistry 24:6740-6743.
23. Smith VL, Doyle KE, Maune JF, Munjaal RP, Beckingham K. 1987. Structure and sequence of the Drosophila melanogaster calmodulin gene. J MoI Biol 196:471-485.
24. Starovasnik MA, Su DR, Beckingham K, Klevit RE. 1992. A series of point mutations reveal interactions between the calcium-binding sites of calmodulin. Protein Sci 1:245-253.
25. Strynadka NCJ, James MNG. 1988. Two trifluoperazine-binding sites on calmodulin predicted from comparative molecular modeling with troponin-C. Proteins Struct Fund Genet 3:1-17.
26. Tartof KD, Hobbs CA. 1987. Improved media for growing plasmid and cosmid clones. Bethesda Res Labs Focus 9:12-17.
27. Taylor DA, Sack JS, Maune JF, Beckingham K, Quiocho FA. 1991. Structure of a recombinant calmodulin from Drosophila melanogaster refined at 2.2 Å resolution. J Biol Chem 266:21375-21380.
28. Törok K, Lane AN, Martin SR, Janot JM, Bayley PM. 1992. Effects of calcium binding on the internal dynamic properties of bovine brain calmodulin, studied by NMR and optical spectroscopy. Biochemistry 31: 3452-3462.
29. VanBerkum MRA, Means AR. 1991. Three amino acid substitutions in domain-1 of calmodulin prevent the activation of chicken smooth muscle myosin light chain kinase. J Biol Chem 266:21488-21495.
30. Zhang M, Tanaka T, Ikura M. 1995. Calcium-induced conformational transition revealed by the solution structure of apo calmodulin. Nature Struct Biol 2:758-767.