A rapid and sensitive fluorometric screening assay using YO-PRO-1 to quantify tumour cell invasion through Matrigel

Clinical and Experimental Metastasis

Volumn 14, Issue 5, 1996, Pages 451-458

  Gohla, Antje ^a   Eckert, Klaus ^a   Maurer, H Rainer ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

DNA; Fluorometric screening assay; Invasion; Tumour cells; YO PRO 1

Indexed keywords

DNA; DYE; MATRIGEL;

ARTICLE; CANCER INVASION; CONTROLLED STUDY; FLUOROMETRY; HUMAN; HUMAN CELL; NONHUMAN; SCREENING TEST; TUMOR CELL;

BASEMENT MEMBRANE; BENZOXAZOLES; COLLAGEN; DNA, NEOPLASM; DRUG COMBINATIONS; FLUORESCENT DYES; HUMANS; LAMININ; METHODS; NEOPLASM INVASIVENESS; PROTEOGLYCANS; QUINOLINIUM COMPOUNDS; TIME FACTORS; TUMOR CELLS, CULTURED;

EID: 0029972246     PISSN: 02620898     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00128961     Document Type: Article

References (29)

1. Liotta LA, 1984, Tumor invasion and metastases: role of the basement membrane. Am J Pathol, 117, 339-48.
2. Terranova VP, Liotta LA, Russo RG and Martin GR, 1982, Role of laminin in the attachment and metastasis of murine tumor cells. Cancer Res, 42, 2265-9.
3. Jones PA and DeClerck YA, 1980, Destruction of extracellular matrices containing glycoproteins, elastin and collagen by metastatic human tumor cells. Cancer Res, 40, 3222-7.
4. Kramer RH and Vogel KG, 1984, Selective degradation of basement membrane macromolecules by metastatic melanoma cells. J Natl Cancer Inst, 72, 889-97.
5. Turpeenniemi-Hujanen T, Thorgeirsson UP, Rao CN, and Liotta LA, 1986, Laminin increases the release of type IV collagenase from malignant cells. J Biol Chem, 261, 1883-9.
6. Mignatti P, Robbins E and Rifkin DB, 1986, Tumor invasion through the human amniotic membrane: requirement for a proteinase cascade. Cell, 47, 487-98.
7. Hujanen ES and Terranova VP, 1985, Migration of tumor cells to organ-derived chemoattractants, Cancer Res, 45, 3517-21.
8. Poste G, Doll J, Hart IR and Fidler IJ, 1980, In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties. Cancer Res, 40, 1636-44.
9. Liotta LA, Lee WC and Morakis DJ, 1980, New method for preparing large surfaces of intact basement membrane for tumor invasion studies. Cancer Lett, 11, 141-7.
10. Hart IR and Fidler IJ, 1978, An in vitro quantitative assay for tumor cell invasion. Cancer Res, 38, 3218-24.
11. Starkey JR, Hosick HL, Stanford DR and Liggitt HD, 1984, Interaction of metastatic tumor cells with bovine lens capsule basement membrane. Cancer Res, 44, 1585-94.
12. Terranova VP, Hujanene ES, Loeb DM et al. 1986, Use of a reconstituted basement membrane to measure cell invasiveness and select for highly invasive tumor cells. Proc Natl Acad sci USA, 83, 465-9.
13. Albini A, Iwamoto Y, Kleinman HK et al, 1987, A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res, 47, 3239-45.
14. Repesh LA, 1989. A new in vitro assay for quantitating tumor cell invasion. Invasion Metastasis, 9, 192-208.
15. Parish CR, Jacobsen KB, and Coombe DR, 1992, A basement-membrane permeability assay which correlates with the metastatic potential of tumour cells. Int J Cancer, 52, 378-83.
16. Mosmann T, 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth, 65, 55-63.
17. Schlechte W, Brattain M and Boyd D, 1990, Invasion of extracellular matrix by cultured colon cancer cells: dependence on urokinase receptor display. Cancer Commun, 2, 173-9.
18. Imamura H, Takao S and Aikou T, 1994, A modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenylte-trazolium bromide assay for quantitating tumor cell invasion. Cancer Res, 54, 3620-4.
19. Rago R, Mitchen J and Wilding G, 1990, DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water. Anal Biochem, 191, 31-4.
20. Adams CJ and Storrie B, 1981, A simple DNA-dependent fluorescence enhancement assay for cell number. J Histochem Cytochem, 29, 326.
21. Blaheta RA, Franz M, Auth MKH, Wenisch HJC and Markus BH, 1991. A rapid non-radioactive fluorescence assay for the measurement of both cell number and proliferation. J Immunol Meth, 142, 199-206.
22. Wan CP, Sigh RV and Lau BHS, 1994, A simple fluorometric assay for the determination of cell numbers. J Immunol Meth, 173, 265-72.
23. Haughland RP, 1992, Nucleic acid stains. In: Larison KD, ed. Handbook of Fuorescent Probes and Research Chemicals, 5th edition. Eugene, OR, Molecular Probes, pp. 221-9.
24. Grotendorst GR, 1987, Spectrophotometric assay for the quantitation of cell migration in the Boyden chamber chemotaxis assay. Methods Enzvmol, 147, 144-52.
25. Kinsella AR, Lepts GC, Hill CL and Jones M, 1994, Reduced E-cadherin expression correlates with increased invasiveness in colorectal carcinoma cell lines. Clin Exp Metastasis, 12, 335-42.
26. Hollas W, Blasi F and Boyd D, 1991, Role of the urokinase receptor in facilitating extracellular matrix invasion by cultured colon cancer. Cancer Res, 51, 3690-5.
27. Price JE, Polyzos A, Zhang RD and Daniels LM, 1990, Tumorigenicity and metastasis of human breast carcinoma cell lines in nude mice. Cancer Res, 50, 717-21.
28. Fraker LD, Halter SA and Forbes JT, 1984, Growth inhibition by retinol of a human breast carcinoma cell line in vitro and in athymic mice. Cancer Res, 44, 5757-63.
29. de Vries JE, Dinjens WNM, De Bruyne GK, et al. 1995, In vivo and in vitro invasion in relation to phenotypic characteristics of human colorectal carcinoma cells. Br J Cancer, 71, 271-7.