Effect of alkalizing agents on the processing of the β-amyloid precursor protein

Brain Research

Volumn 716, Issue 1-2, 1996, Pages 91-100

  Schrader Fischer, Gesine ^a   Paganetti, Paolo A ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

alkalizing agent; Alzheimer's disease; amyloid precursor protein; endocytosis; intracellular compartment; processing; amyloid

Indexed keywords

AMMONIUM CHLORIDE; AMYLOID BETA PROTEIN; BAFILOMYCIN; CHLOROQUINE;

ALZHEIMER DISEASE; ARTICLE; CELL LINE; CONTROLLED STUDY; ENDOCYTOSIS; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PH; PRIORITY JOURNAL; PROTEIN PROCESSING;

EID: 0029972129     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/0006-8993(96)00002-9     Document Type: Article

References (44)

1. Bowman, E.J., Siebers, A. and Altendorf, K., Bafilomycins: A class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells, Proc. Natl. Acad. Sci. USA, 85 (1988) 7972-7976.
2. Busciglio, J., Gabuzda, D.H., Matsudaira, P. and Yankner, B.A., Generation of β-amyloid in the secretory pathway in neuronal and nonneuronal cells. Proc. Natl. Acad. Sci. USA, 90 (1993) 2092-2096.
3. Cai, X.-D., Golde, T.E. and Younkin, S.G., Release of excess amyloid β protein from a mutant amyloid β protein precursor, Science, 259 (1993) 514-516.
4. Chen, C. and Okayama, H., High-efficiency transformation of mammalian cells by plasmid DNA, Mol. Cell Biol., 7 (1987) 2745-2752.
5. Citron, M., Oltersdorf, T., Haass, C., McConlogue, L., Hung, A.Y., Seubert, P., Vigo-Pelfrey, C., Lieberburg, I. and Selkoe, D.J., Mutation of the β-amyloid precursor protein in familial Alzheimer's disease increases β-protein production. Nature, 360 (1992) 672-674.
6. Citron, M., Vigo-Pelfrey, C., Teplow, D.B., Miller, C., Schenk, D., Johnston, J., Winblad, B., Venizelos, N., Lannfelt, L. and Selkoe, D.J., Excessive production of amyloid β-protein by peripheral cells of symptomatic and presymptomatic patients carrying the Swedish familial Alzheimer disease mutation, Proc. Natl. Acad. Sci. USA, 91 (1994) 11993-11997.
7. Citron, M., Teplow, D.B. and Selkoe, D.J., Generation of amyloid β-protein from its precursor is sequence specific, Neuron, 14 (1995) 661-670.
8. De Strooper, B., Umans, L., Van Leuven, F. and Van den Berghe, H., Study of the synthesis and secretion of normal and artificial mutants of murine amyloid precursor protein (APP): Cleavage of APP occurs in a late compartment of the default secretion pathway, J. Cell Biol., 121 (1993) 295-304.
9. De Duve, C., De Barsy, T., Poole, B., Trouet, A., Tulkens, P. and Van Hoof, F., Lysosomotropic agents, Biochem. Pharmacol., 23 (1974) 2495-2531.
10. Dyrks, T., Dyrks, E., Mönning, U., Urmoneit, B., Turner, J. and Beyreuther, K., Generation of βA4 from the amyloid protein precursor and fragments thereof, FEBS Lett., 335 (1993) 89-93.
11. Esch, F.S., Keim, P.S., Beattle, E.C., Blacher, R.W., Culwell, A.R., Oltersdorf, T., McClure, D. and Ward, P.J., Cleavage of amyloid β peptide during constitutive processing of its precursor, Science, 248 (1990) 1122-1124.
12. Fuller, S.J., Storey, E., Li, Q.-X., Smith, A.I., Beyreuther, K. and Masters, C.L., Intracellular production of βA4 amyloid of Alzheimer's disease: Modulation by phosphoramidon and lack of coupling to the secretion of the amyloid precursor protein. Biochemistry; 34 (1995) 8091-8098.
13. m of albumin receptor-mediated endocytosis in OK cells, Am. J. Physiol. Renal, Fluid Electrolyte Physiol., 268 (1995) F899-F906.
14. Golde, T.E., Estus, S., Younkin, L.H., Selkoe, D.J. and Younkin, S.G., Processing of the amyloid protein precursor to potentially amyloidogenic derivatives, Science, 255 (1992) 728-730.
15. Haass, C., Schlossmacher, M.G., Hung, A.Y., Vigo-Pelfrey, C., Mellon, A., Ostaszewski, B.L., Lieberburg, I., Koo, E.H., Schenk, D., Teplow, D.B. and Selkoe, D.J., Amyloid β-peptide is produced by cultured cells during normal metabolism, Nature, 359 (1992) 322-325.
16. Haass, C., Koo, E.H., Mellon, A., Hung, A.Y. and Selkoe, D.J., Targeting of cell-surface β-amyloid precursor protein to lysosomes: Alternative processing into amyloid-bearing fragments, Nature, 357 (1992) 500-503.
17. Haass, C., Hung, A.Y., Schlossmacher, M.G., Teplow, D.B. and Selkoe, D.J., β-amyloid peptide and a 3-kDa fragment are derived by distinct cellular mechanisms, J. Biol. Chem., 268 (1993) 3021-3024.
18. --ATPase inhibitor bafilomycin A1 differentially affects proteolytic processing of mutant and wild-type β-amyloid precursor protein, J. Biol. Chem., 270 (1995) 6186-6192.
19. Haass, C., Hung, A.Y., Citron, M., Teplow, D.B. and Selkoe, D.J., β-amyloid, protein processing and Alzheimer's disease. Drug Res., 45 (1995) 398-402.
20. Hardy, J. and Allsop, D., Amyloid deposition as the central event in the aetiology of Alzheimer's disease, Trends Pharmacol. Sci., 12 (1991) 383-388.
21. Johnston, J.A., Cowburn, R.F., Norgren, S., Wiehager, B., Venizelos, N., Winblad, B., Vigo-Pelfrey, C., Schenk, D., Lannfelt, L. and O'Neill, C., Increased β-amyloid release and levels of amyloid precursor protein (APP) in fibroblast cell lines from family members with the Swedish Alzheimer's disease APP670/671 mutation, FEBS Lett., 354 (1994) 274-278.
22. Kang, J., Lemaire, H.G., Unterbeck, A., Salbaum, J.M., Masters, C.L., Grzeschik, K.H., Multhaup, G., Beyreuther, K. and Mueller-Hill, B., The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature, 325 (1987) 733-736.
23. Knops, J., Suomensaari, S., Lee, M., McConlogue, L., Seubert, P. and Sinha, S., Cell-type and amyloid precursor protein-specific inhibition of A β release by bafilomycin A1, a selective inhibitor of vacuolar ATPases, J. Biol. Chem., 270 (1995) 2419-2422.
24. Koo, E.H. and Squazzo, S.L., Evidence that production and release of amyloid β-protein involves the endocytic pathway, J. Biol. Chem., 269 (1994) 17386-17389.
25. Lai, A., Sisodia, S.S. and Trowbridge, I.S., Characterization of sorting signals in the β-amyloid precursor protein cytoplasmic domain, J. Biol. Chem., 270 (1995) 3565-3573.
26. Martin, B.L., Schrader-Fischer, G., Busciglio, J., Duke, M., Paganetti, P. and Yankner, B.A., Intracellular accumulation of β amyloid in cells expressing the Swedish mutant amyloid precursor protein, submitted.
27. Nordstedt, C., Caporaso, G.L., Thyberg, J., Gandy, S.E. and Greengard, P., Identification of the Alzheimer β/A4 amyloid precursor protein in clathrin-coated vesicles purified from PC12 cells, J. Biol. Chem., 268 (1993) 608-612.
28. Paganetti, P. and Scheller, R.H., Proteolytic processing of the Aplysia A peptide precursor in AtT-20 cells, Brain Res., 633 (1994) 53-62.
29. Palacios, G., Palacios, J.M., Mengod, G. and Frey, P., β-amyloid precursor protein localization in the Golgi apparatus in neurons and oligodendrocytes. An immunocytochemical structural and ultrastructural study in normal and axotomized neurons, Mol. Brain Res., 15 (1992) 195-206.
30. Price, K.M., Cuthbertson, A.S., Varndell, I.M. and Sheppard, P.W., The production and characterization of monoclonal antibodies to myc, c-erbB-2 and EFG-receptor using a synthetic peptide approach, Dev. Biol. Stand., 71 (1990) 23-31.
31. Sapirstein, V.S., Durrie, R., Berg, M.J. and Marks, N., Amyloid precursor protein is enriched in axolemma and periaxolemmal-myelin and clathrin-coated vesicles, J. Neurosci. Res. (1994) 348-358.
32. Schaegger, H. and von Jagow, G., Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa, Anal. Biochem., 166 (1987), 368-379.
33. Selkoe, D.J., Normal and abnormal biology of the β-amyloid precursor protein, Annu. Rev. Neurosci., 17 (1994) 489-517.
34. Seubert, P., Vigo-Pelfrey, C., Esch, F., Lee, M., Dovey, H., Davis, D., Sinha, S., Schlossmacher, M., Whaley, J., Swindlehurst, C., McCormack, R., Wolfert, R., Selkoe, D.J., Lieberburg, I. and Schenk, D., Isolation and quantification of soluble Alzheimer's β-peptide from biological fluids, Nature, 359 (1992) 325-327.
35. Shoji, M., Golde, T.E., Ghiso, J., Cheung, T.T., Estus, S., Shaffer, L.M., Cai, X.D., McKay, D.M., Tintner, R., Frangione, B. and Younkin, S.G., Production of the Alzheimer amyloid β protein by normal proteolytic processing, Science, 258 (1992) 126-129.
36. Siman, R., Mistretta, S., Durkin, J.T., Savage, M.J., Loh, T., Trusko, S. and Scott, R.W., Processing of the β-amyloid precursor. Multiple proteases generate and degrade potentially amyloidogenic fragments, J. Biol. Chem., 268 (1993) 16602-16609.
37. Tagawa, K., Yazaki, M., Kinouchi, T., Maruyama, K., Sorimachi, H., Tsuchiya, T., Suzuki, K. and Ishiura, S., Amyloid precursor protein is found in lysosomes, Gerontology, 39 (suppl.) (1993) 24-29.
38. Tsuzuki, K., Fukatsu, R., Takamaru, Y., Fujii, N. and Takahata, N., Potentially amyloidogenic fragment of 50 kDa and intracellular processing of amyloid precursor protein in cells cultured under leupeptin, Brain Res., 659 (1994) 213-220.
39. +-ATPase, J. Biol. Chem., 265 (1990) 21940-21945.
40. Van Weert, A.W.M., Dunn, K.W., Geuze, H.J., Maxfield, F.R., and Stoorvogel, W., Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump, J. Cell Biol. , 130 (1995) 821-834.
41. Wertkin, A.M., Turner, R.S., Pleasure, S.J., Golde, T.E., Younkin, S.G., Trojanowski, J.Q., Lee, V.M.-Y., Human neurons derived from a teratocarcinoma cell line express solely the 695-amino acid amyloid precursor protein and produce intracellular β-amyloid or A4 peptides, Proc. Natl. Acad. Sci. USA, 90 (1993) 9513-9517.
42. Wiltfang, J., Arold, N. and Neuhoft, V., A new multiphasic buffer system for sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins and peptides with molecular masses 100000-1000, and their detection with picomolar sensitivity, Electrophoresis, 12 (1991) 352-366.
43. Xu, H. and Shields, D., Prosomatostatin processing in permeabilized cells, J. Biol. Chem., 36 (1994) 22875-22881.
44. +-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells, J. Biol. Chem., 266 (1991) 17707-17712.