Enhanced expression of endochitinase in Trichoderma harzianum with the cbh1 promoter of Trichoderma reesei

Applied and Environmental Microbiology

Volumn 62, Issue 6, 1996, Pages 2152-2155

  Margolles Clark, Emilio ^a   Harman, Gary E ^b   Penttilä, Merja ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

^b Department of Obstetrics Gynecology   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CHITINASE; FUNGAL PROTEIN;

ARTICLE; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE EXPRESSION REGULATION; HYPHOMYCETES; NONHUMAN; PROMOTER REGION; TRANSCRIPTION REGULATION;

EID: 0029971109     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.6.2152-2155.1996     Document Type: Article

References (18)

1. Carsolio, C., A. Gutiérrez. B. Jiménez, M. Van Montagu, and A. Herrera-Estrella. 1994. Characterization of ech-42, a Trichoderma harzianum endochitinase gene expressed during mycoparasitism. Proc. Natl. Acad. Sci. USA 91:10903-10907.
2. Chet, J. 1987. Trichoderma - application, mode of action, and potential as a biocontrol agent of soilborne plant pathogenic fungi, p. 137-160. In I. Chet (ed.), Innovative approaches to plant disease control. John Wiley & Sons, New York.
3. Claydon, N., M. Allan, J. R. Hanson, and A. G. Avent. 1987. Antifungal alkyl pyrones of Trichoderma harzianum. Trans. Br. Mycol. Soc. 88:503-513.
4. García, I., J. M. Lora, J. de la Cruz, T. Benítez, A. Llobell, and J. A. Pintor-Toro. 1994. Cloning and characterization of a chitinase (CHIT42) cDNA from the mycoparasitic fungus Trichoderma harzianum. Curr. Genet. 27:83-89.
5. Hayes, C. K., S. Klemsdal, M. Lorito, A. Di Pietro, C. Peterbauer, J. P. Nakas, A. Tronsmo, and G. E. Harman. 1994. Isolation and sequence of an endochitinase-encoding gene from a cDNA library of Trichoderma harzianum. Gene 138:143-148.
6. Ilmén, M., A. Saloheimo, M.-L. Onnela, and M. Penttilä. Regulation of cellulase expression in the filamentous fungus Trichoderma reesei. Submitted for publication.
7. Ilmén, M., C. Thrane, and M. Penttilä. The glucose represor gene cre1 of Trichoderma reesei: isolation and expression of a full length and a truncated mutant gene. Mol. Gen. Genet., in press.
8. Inbar, J., and I. Chet. 1992. Biomimics of fungal cell-cell recognition by use of lectin-coated nylon fibers. J. Bacteriol. 174:1055-1059.
9. Keränen, S., and M. Penttilä. 1995. Production of recombinant proteins in the filamentous fungus Trichoderma reesei. Curr. Opin. Biotechnol. 6:534-537.
10. Lorito, M., G. E. Harman, C. K. Hays, R. M. Broadway, A. Tronsmo, S. L. Woo, and A. Di Pietro. 1993. Chitinolytic enzymes produced by Trichoderma harzianum: antifungal activity of purified endochitinase and chitobiosidase. Phytopathology 83:302-307.
11. Lorito, M., C. K. Hayes, A. Di Pietro, S. L. Woo, and G. E. Harman. 1994. Purification, characterization, and synergistic activity of a glucan β1,3-glucusidase and an N-acetyl-β-glucosaminidase from Trichoderma harzianum. Phytopathology 84:398-405.
12. Margolles-Clark, E., C. K. Hayes, G. E. Harman, and M. Penttilä. 1996. Improved production of Trichoderma harzianum endochitinase by expression in Trichoderma reesei. Appl. Environ. Microbiol. 62:2145-2151.
13. Nyyssönen, E., M. Penttilä, A. Harkki, A. Saloheimo, J. K. C. Knowles, and S. Keränen. 1993. Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei. Bio/Technology 11:591-595.
14. Penttilä, M., H. Nevalainen, M. Rättö, E. Salminen, and J. K. C. Knowles. 1987. A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61:155-164.
15. Penttilä, M., A. Saloheimo, M. Ilmén, and M.-L. Onnela. 1993. Regulation of the expression of Trichoderma cellulases at mRNA and promoter level, p. 189-197. In P. Suominen and T. Reinikainen (ed.), Proceedings of the 2nd TRICEL Symposium on Trichoderma reesei Cellulases and Other Hydrolases. Fundation for Biotechnical and Industrial Fermentation Research, Espoo, Finland.
16. Schirmböck, M,. M. Lorito, Y. Wang, C. K. Hayes, I. Arisan-Atac, F. Scala, G. E. Harman, and C. P. Kubicek. 1994. Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Appl. Environ. Microbiol. 60:4364-4370.
17. Tronsmo, A., and G. E. Harman. 1992. Coproduction of chitinolytic enzymes and biomass for biological control by Trichoderma harzianum on media containing chitin. Biol. Control 2:272-277.
18. Ulhoa, C. C., and J. F. Peberdy. 1991. Regulation of chitinase synthesis in Trichoderma harzianum. J. Gen. Microbiol. 137:2163-2169.