Protein kinase C mediates up-regulation of urokinase and its receptor in the migrating keratinocytes of wounded cultures, but urokinase is not required for movement across a substratum in vitro

Journal of Cellular Physiology

Volumn 167, Issue 3, 1996, Pages 500-511

  Ando, Yoshihiro ^a   Jensen, Pamela J ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 [1 (3 AMIDINOTHIOPROPYL) 1H INDOL 3 YL] 3 (1 METHYL 1H INDOL 3 YL)MALEIMIDE; AMPHIREGULIN; BASIC FIBROBLAST GROWTH FACTOR; BRYOSTATIN; CELL SURFACE RECEPTOR; CYCLOHEXIMIDE; DACTINOMYCIN; EPIDERMAL GROWTH FACTOR; PROTEIN KINASE C; STAUROSPORINE; TRANSFORMING GROWTH FACTOR; TUMOR NECROSIS FACTOR ALPHA; UROKINASE;

ARTICLE; CELL CULTURE; CELL MIGRATION; CYTOSKELETON; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; NEWBORN; PRIORITY JOURNAL; PROTEIN EXPRESSION; WOUND;

ANTIBODIES; CELL MOVEMENT; CELLS, CULTURED; CYCLOHEXIMIDE; DACTINOMYCIN; ENZYME ACTIVATION; HUMANS; IMMUNOHISTOCHEMISTRY; KERATINOCYTES; LACTONES; MACROLIDES; MITOGENS; PLASMINOGEN; PROTEIN KINASE C; RECEPTORS, CELL SURFACE; TRYPSIN INHIBITOR, KUNITZ SOYBEAN; UP-REGULATION; URINARY PLASMINOGEN ACTIVATOR;

EID: 0029970958     PISSN: 00219541     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4652(199606)167:3<500::AID-JCP14>3.0.CO;2-7     Document Type: Article

References (77)

1. Albrecht-Buehler, G. (1977) The phagokinetic tracks of 3T3 cells. Cell, 11:395-404.
2. Ando, Y., and Jensen, P. J. (1993) Epidermal growth factor and insulin-like growth factor I enhance keratinocyte migration. J. Invest. Dermatol., 100:633-639.
3. Ando, Y., Lazarus, G.S., and Jensen, P.J. (1993) Activation of protein kinase C inhibits human keratinocyte migration. J. Cell. Physiol., 156:487-496.
4. Azzi, A., Boscoboinik, D., and Hensey, C. (1992) The protein kinase C family. Eur. J. Biochem., 208:547-557.
5. Blasi, F. (1993) Urokinase and urokinase receptor: A paracrine/autocrine system regulating cell migration and invasiveness. BioEssays. 15:105-111.
6. Blasi, F., Vassalli, J.D., and Danø, K. (1987) Urokinase-type plasminogen activator: Proenzyme, receptor, and inhibitors. J. Cell Biol., 104:801-804.
7. Botteri, F.M., Ballmer-Hofer, K., Rajput, B., and Nagamine, Y. (1990) Disruption of cytoskeletal structures results in the induction of the urokinase-type plasminogen activator gene expression. J. Biol. Chem., 265:13327-13334.
8. Boyce, S.T., and Ham, R.G. (1983) Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J. Invest. Dermatol., 81:33s-40s.
9. Brown, J.M., and Chambers, D.A. (1984) Effects of biological response modifiers on plasminogen activator activity in epidermal cell culture. Br. J. Dermatol. Suppl., 27:252-256.
10. Bürk, R.R. (1973) A factor from a transformed cell line that affects cell migration. Proc. Natl. Acad. Sci. U.S.A., 70:369-372.
11. Camiolo, S.M., Siuta, M.R., and Madeja, J.M. (1982) Improved medium for extraction of plasminogen activator from tissue. Prep. Biochem., 12:297-305.
12. Coffey, R.J., Derynck, R., Wilcox, J.N., Bringman, T.S., Goustin, A.S., Moses, H.L., and Pittelkow, MR. (1987) Production and auto-induction of transforming growth factor-α in human keratinocytes. Nature, 325:817-820.
13. Coleman, P.L., and Green, G.D.J. (1981) A sensitive, coupled assay for plasminogen activator using a thiol ester substrate for plasmin. Ann. N.Y. Acad. Sci., 370:617-626.
14. Conese, M., and Blasi, F. (1995) Urokinase/urokinase receptor system. Internalization/degradation of urokinase-serpin complexes: Mechanism and regulation. Biol. Chem. Hoppe-Seyler, 376:143-155.
15. Cook, P.W., Mattox, P.A., Keeble, W.W., Pittelkow, M.R., Plowman, G.D., Shoyab, M., Adelman, J.P., and Shipley, G.D. (1991) A heparin sulfate-regulated human keratinocyte autocrine factor is similar or identical to amphiregulin. Mol. Cell Biol., 11:2547-2557.
16. Danø, K., Andreasen, P.A., Grøndahl-Hansen, J., Kristensen, P., Nielsen, L.S., and Skriver, L. (1985) Plasminogen activators, tissue degradation, and cancer. Adv. Cancer Res., 44:139-266.
17. Danø, K., Behrendt, N., Rønne, E., Ellis, V., and Blasi, F. (1990) The urokinase receptor and regulation of pericellular plasminogen activation. In: Molecular Biology of the Cardiovascular System. Alan R. Liss, Inc., New York, pp. 173-186.
18. Degen, J.L., Estensen, R.D., Nagamine, Y., and Reich, E. (1985) Induction and desensitization of plasminogen activator gene expression by tumor promoters. J. Biol. Chem., 260:12426-12433.
19. Del Rosso, M., Fibbi, G., Dini, G., Grappone, C., Pucci, M., Caldini, R., Magnelli, L., Fimiani, M., Lotti, T., and Panconesi, E. (1990) Role of specific membrane receptors in urokinase-dependent migration of human keratinocytes. J. Invest. Dermatol., 94:310-316.
20. Del Rosso, M., Anichini, E., Pedersen, N., Blasi, F., Fibbi, G., Pucci, M., and Ruggiero, M. (1993) Urokinase-urokinase receptor interaction: Non-mitogenic signal transduction in human epidermal cells. Biochem. Biophys. Res. Commun., 190:347-352.
21. Deutsch, D.G., and Mertz, E.T. (1970) Plasminogen: Purification from human plasma by affinity chromatography. Science, 170:1095-1096.
22. Dignass, A.U., and Podolsky, D.K. (1993) Cytokine modulation of intestinal epithelial cell restitution: Central role of transforming growth factor β. Gastroenterology, 105:1323-1332.
23. Dignass, A.U., Tsunekawa, S., and Podolsky, D.K. (1994) Fibroblast growth factors modulate intestinal epithelial cell growth and migration. Gastroenterology, 106:1254-1262.
24. Gabbiani, G., Gabbiani, F., Heimark, R.L., and Schwartz, S.M. (1984) Organization of actin cytoskeleton during early endothelial regeneration in vitro. J. Cell Sci., 66:39-50.
25. Gospodarowicz, D., Cheng, J., Lui, G.M., Baird, A., and Böhlent, P. (1984) Isolation of fibroblast growth factor by heparin-sepharose affinity chromatography: Identity with pituitary fibroblast growth factor. Proc. Natl. Acad. Sci. U.S.A. 87:6963-6967.
26. Gottlieb, A.I., May, L.M., Subrahmanyan, L., and Kalnins, V.I. (1981) Disruption of microtubule organizing centers in migrating sheets of endothelial cells. J. Cell Biol., 91:589-594.
27. Grinnell, F. (1992) Wound repair, keratinocyte activation, and integrin modulation. J. Cell Sci., 101:1-5.
28. Grøndahl-Hansen, J., Lund, L.R., Ralfkicer, E., Ottevanger, V., and Danø, K. (1988) Urokinase- and tissue-type plasminogen activators in keratinocytes during wound reepithelialization in vivo. J. Invest. Dermatol., 90:790-795.
29. Halaban, R., Langdon, R., Birchall, N., Cuono, C., Baird, A., Scott, G., Moellmann, G., and McGuire, J. (1988) Basic fibroblast factor from human keratinocytes is a natural mitogen for melanocytes. J. Cell Biol., 107:1611-1619.
30. Hug, H., and Sarre, T.F. (1993) Protein kinase C isozymes: Divergence in signal transduction. Biochem. J., 291:329-343.
31. Jensen, P.J., and Wheelock, M.J. (1992) Regulation of urokinase plasminogen activator localization in keratinocytes by calcium iron and E-cadherin. Exp. Cell Res., 202:190-198.
32. Jensen, P.J., Baird, J., Morioka, S., Lessin, S., and Lazarus, G.S. (1988) Epidermal plasminogen activator is abnormal in cutaneous lesions. J. Invest. Dermatol., 90:777-782.
33. Jensen, P.J., John, M., and Baird, J. (1990) Urokinase and tissue type plasminogen activators in human keratinocyte culture. Exp. Cell Res., 187:162-169.
34. Jensen, P.J., Wu, Q., Janowitz, P., Ando, Y., and Schechter, N.M. (1995) Plasminogen activator inhibitor type 2: An intracellular keratinocyte differentiation product that is incorporated into the cornified envelope. Exp. Cell Res., 217:65-71.
35. Jones, K.T., and Sharpe, G.R. (1994) Staurosporine, a non-specific PKC inhibitor, induces keratinocyte differentiation and raises intracellular calcium, but Ro31-8220, a specific inhibitor, does not. J. Cell. Physiol., 159:324-330.
36. Kariko, K., Kuo, A., Boyd, D., Okada, S., Cines, D.B., and Barnathan, E.S. (1993) Overexpression of urokinase receptor increases matrix invasion without altering cell migration in a human osteosarcoma cell line. Cancer Res., 53:3109-3117.
37. Kawada, M., and Umezawa, K. (1995) Suppression of in vitro invasion of human fibrosarcoma cells by a leupeptin analogue inhibiting the urokinase-plasmin system. Biochem. Biophys. Res. Commun., 209:25-30.
38. Klein, S.B., Fisher, G.J., Jensen, T.C., Mendelsohn, J., Voorhees, J.J., and Elder, J.T. (1992) Regulation of TGF-α expression in human keratinocytes: PKC-dependent and -independent pathways. J. Cell. Physiol., 151:326-336.
39. Kock, A., Schwartz, T., Kirnbauer, R., Urbanski, A., Perry, P., Ansel, J.C., and Luger, T.A. (1990) Human keratinocytes are a source for tumor necrosis factor α: Evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. J. Exp. Med., 172:1609-1614.
40. Kramer, M.D., Schaefer, B., and Reinartz, J. (1995) Plasminogen activation by human keratinocytes: Molecular pathways and cell-biological consequences. Biol. Chem. Hoppe-Seyler, 376:131-141.
41. Krawczyk, W.S. (1971) A pattern of epidermal cell migration during wound healing. J. Cell Biol., 49:257-263.
42. Langer, D.L., Kuo, A., Kariko, K., Ahuja, M., Klugherz, B.D., Ivanics, K.M., Hoxie, J.A., Williams, W.V., Liang, B.T., Cines, D.B., and Barnathan, E.S. (1993) Regulation of the endothelial cell urokinase-type plasminogen activator receptor. Evidence for cyclic AMP-dependent and protein kinase C-dependent pathways. Circ. Res., 72:330-340.
43. Liu, G., Shuman, M.A., and Cohen, R.L. (1995) Co-expression of urokinase, urokinase receptor and PAI-1 is necessary for optimum invasiveness of cultured lung cancer cells. Int. J. Cancer, 60:501-506.
44. Lund, L.R., Ellis, V., Rønne, E., Pyke, C., and Danø, K. (1995) Transcriptional and post-translational regulation of the receptor for urokinase-type plasminogen activator by cytokines and tumour promoters in the human lung carcinoma cell line A549. Biochem. J., 310:345-352.
45. Mansbridge, J.N., and Knapp, A.M. (1987) Changes in keratinocyte maturation during wound healing. J. Invest. Dermatol., 89:253-263.
46. Mawatari, M., Okamura, K., Matsuda, T., Hamanaka, R., Mizoguchi, H., Higashio, K., Kohno, K., and Kuwano, M. (1991) Tumor necrosis factor and epidermal growth factor modulate migration of human microvascular endothelial cells and production of tissue-type plasminogen activator and its inhibitor. Exp. Cell Res., 192:574-580.
47. McNeill, H., and Jensen, P. J. (1990) A high-affinity receptor for urokinase plasminogen activator on human keratinocytes: Characterization and potential modulation during migration. Cell Regul., 1:843-852.
48. Meissauer, A., Kramer, M.D., Hofmann, M., Erkell, L.J., Jocob, E., Schirrmacher, V., and Brunner, G. (1991) Urokinase-type and tissue-type plasminogen activators are essential for in vitro invasion of human melanoma cells. Exp. Cell Res., 192:453-459.
49. Mignatti, P. (1995) Extracellular matrix remodeling by metalloproteinases and plasminogen activators. Kidney Int., 47(Suppl. 49):S12-S14.
50. Mira-Y-Lopez, R. (1991) Retinoic acid priming potentiates the induction of urokinase-type plasminogen activator by cyclic adenosine monophosphate in mouse mammary carcinoma cells. J. Cell. Physiol., 147:46-54.
51. Morioka, S., Jensen, P.J., and Lazarus, G.S. (1985) Human epidermal plasminogen activator: Characterization, localization, and modulation. Exp. Cell Res., 161:364-372.
52. Morioka, S., Lazarus, G.S., Baird, J.L., and Jensen, P.J. (1987) Migrating keratinocytes express urokinase-type plasminogen activator. J. Invest. Dermatol., 88:418-423.
53. Murthy, U., Basu, A., Rodeck, U., Herlyn, M., Ross, A.H., and Das, M. (1987) Binding of an antagonistic monoclonal antibody to an intact and fragmented EGF-receptor polypeptide. Arch. Biochem. Biophys., 252:549-560.
54. Murthy, U., Rieman, D.J., and Rodeck, U. (1990) Inhibition of TGF-α induced signal transduction and cell proliferation by anti-EGF receptor antibody 425. Biochem. Biophys. Res. Commun., 172:471-476.
55. Niedbala, M.J., and Stein-Picarella, M. (1993) Role of protein kinase C in tumor necrosis factor induction of endothelial cell urokinase-type plasminogen activator. Blood, 81:2608-2617.
56. Nixon, J.S., Bishop, J., Bradshaw, D., Davis, P.D., Hill, C.H., Elliot, L.H., Kumar, H., Lawton, G., Lewis, E.J., Mulqueen, M., Westmacott, D., Wadsworth, J., and Wilkinson, S.E. (1992) The design and biological properties of potent and selective inhibitors of protein kinase C. Biochem. Soc. Trans., 20:419-425.
57. Odekon, L.E., Sato, Y., and Rifkin, D.B. (1992) Urokinase-type plasminogen activator mediates basic fibroblast growth factor-induced bovine endothelial cell migration independent of its proteolytic activity. J. Cell. Physiol., 150:258-263.
58. Pepper, M.S., Vassalli, J.-D., Montesano, R., and Orci, L. (1987) Urokinase-type plasminogen activator is induced in migrating capillary endothelial cells. J. Cell Biol., 105:2535-2541.
59. Pettit, G.R., Kamano, Y., Herald, C.L., and Tozawa, M. (1985) Isolation and structure of bryostatins 5-7. Can. J. Chem., 63:1204-1208.
60. Pollanen, J., Stephens, R. W., and Vaheri, R. (1991) Directed plasminogen activation at the surface of normal and malignant cells. Adv. Cancer Res., 57:273-238.
61. Reinartz, J., Link, J., Todd, R.F., and Kramer, M.D. (1994) The receptor for urokinase-type plasminogen activator of a human keratinocyte cell line (HaCaT). Exp. Cell Res., 214:486-498.
62. Rømer, J., Lund, L.R., Eriksen, J., Pyke, C., Kristensen, P., and Danø, K. (1994) The receptor for urokinase-type plasminogen activator is expressed by keratinocytes at the leading edge during re-epithelialization of mouse skin wounds. J. Invest. Dermatol., 102:519-522.
63. Rørth, P., Nerlov, C., Blasi, F., and Johnsen, M. (1990) Transcription factor PEA3 participates in the induction of urokinase plasminogen activatory transcription in murine keratinocytes simulated with epidermal growth factor or phorbol-ester. Nucleic Acids Res., 18:5009-5017.
64. Ruegg, U.T., and Burgess, G.M. (1989) Staurosporine, K-252, and UCN-01: Potent but non-specific inhibitors of protein kinases. Trends Pharm. Sci., 10:218-220.
65. Saarialho-Kere, U.K., Vaalamo, M., Airola, K., Mieme, K.-M., Oikarinen, A.I., and Parks, W.C. (1995) Interstitial collagenase is expressed by keratinocytes that are actively involved in reepithelialization in blistering skin diseases. J. Invest. Dermatol., 104:982-988.
66. Sato, Y., and Rifkin, D.B. (1988) Autocrine activities of basic fibroblast growth factor: Regulation of endothelial cell movement, plasminogen activatory synthesis, and DNA synthesis. J. Cell Biol., 107:1199-1205.
67. Shipley, G.D., and Pittelkow, M.R. (1987) Control of growth and differentiation in vitro of human keratinocytes cultured in serum-free medium. Arch. Dermatol., 123:1541a-1544a.
68. Shipley, G.D., Keeble, W.W., Hendrickson, J.E., Coffey, R.J., Jr., and Pittelkow, M.R. (1989) Growth of normal human keratinocytes and fibroblasts in serum-free medium is stimulated by acidic and basic fibroblast growth factor. J. Cell Physiol., 138:511-518.
69. Snyder, R.W., Lenburg, M.E., Seebaum, A.T., and Grabel, L.B. (1992) Disruption of the cytoskeleton-extracellular matrix linkage promotes the accumulation of plasminogen activators in F9 derived parietal endoderm. Differentiation, 50:153-162.
70. Stahl, A., and Mueller, B.M. (1994) Binding of urokinase to its receptor promotes migration and invasion of human melanoma cells in vitro. Cancer Res., 54:3066-3071.
71. Stricklin, G.P., and Nanney, L.B. (1994) Immunolocalization of collagenase and TIMP in healing human burn wounds. J. Invest. Dermatol., 103:488-492.
72. Stricklin, G.P., Li, L., Jancic, V., Wenczak, B.A., and Nanney, L.B. (1993) Localization of mRNAs representing collagenase and TIMP in sections of healing human burn wounds. Am. J. Pathol., 143:1657-1666.
73. ++ dependent protein kinase C. Biochem. Biophys. Res. Commun., 135:397-402.
74. Tanaka, C., and Nishizuka, Y. (1994) The protein kinase C family for neuronal signaling. Annu. Rev. Neurosci., 17:551-567.
75. Vaheri, A., Stephens, R.W., Salonen, E.-M., Pollanen, J., and Tapiovaara, H. (1990) Plasminogen activation at the cell surface-matrix interface. Cell Differ. Dev., 32:255-262.
76. Viziam, C.B., Matoltsy, A.G., and Mescon, H. (1964) Epithelialization of small wounds. J. Invest. Dermatol., 43:499-507.
77. Young, W.C., and Herman, I.M. (1985) Extracellular matrix modulation of endothelial cell shape and motility following injury in vitro. J. Cell Sci., 73:19-32.