Kinetic analysis of rat steroid 5α-reductase activity in prostate and epididymis homogenates at neutral pH: Evidence for type I activity in epididymis

Journal of Steroid Biochemistry and Molecular Biology

Volumn 57, Issue 1-2, 1996, Pages 95-101

  Span, P N ^a   Benraad, Th J ^a   Sweep, C G J ^a   Smals, A G H ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ISOENZYME; STEROID 5ALPHA REDUCTASE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; EPIDIDYMIS; IMMUNOCYTOCHEMISTRY; MALE; NONHUMAN; PROSTATE; RAT;

ANIMALIA;

EID: 0029970283     PISSN: 09600760     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-0760(95)00239-1     Document Type: Article

References (25)

1. Russell D. W. and Wilson J. D.: Steroid 5α-reductase: two genes/two enzymes. A. Rev. Biochem. 63 (1994) 25-61.
2. Farkash Y., Soreq H. and Orly J.: Biosynthesis of catalytically active rat testosterone 5α-reductase in microinjected Xenopus oocytes: evidence for tissue specific differences in translatable mRNA. Proc. Natn. Acad. Sci. 85 (1988) 5824-5828.
3. Andersson S., Bishop R. W. and Russell D. W.: Expression cloning and regulation of steroid 5α-reductase, an enzyme essential for male sexual differentiation. J. Biol. Chem. 264 (1989) 16,249-16,255.
4. Andersson S. and Russell D. W.: Structural and biochemical properties of cloned and expressed human and rat steroid 5α-reductases. Proc. Natn. Acad. Sci. 87 (1990) 3640-3644.
5. Andersson S., Berman D. M., Jenkins E. P. and Russell D. W.: Deletion of steroid 5α-reductase II gene in male pseudohermaphroditism. Nature 354 (1991) 159-161.
6. Jenkins E. P., Andersson S., Imperato-McGinley J., Wilson J. D. and Russell D. W.: Genetic and pharmacological evidence for more than one human steroid 5α-reductase. J. Clin Invest. 89 (1992) 292-300.
7. Normington K. and Russell D. W.: Tissue distribution and kinetic characteristics of rat steroid 5α-reductase isozymes. J. Biol. Chem. 267 (1992) 19,548-19,554.
8. Thigpen A. E., Cala K. M. and Russell D. W.: Characterization of Chinese hamster ovary cell lines expressing human steroid 5α-reductase isozymes. J. Biol. Chem. 268 (1993) 17,404-17,412.
9. Viger R. S. and Robaire B.: Immunocytochemical localization of 4-ene steroid 5α-reductase type I along with rat epididymis during postnatal development. Endocrinology 134 (1994) 2298-2306.
10. Berman D. M. and Russell D. W.: Cell-type-specific expression of rat steroid 5α-reductase isozymes. Proc. Natn. Acad. Sci. 90 (1993) 9359-9363.
11. Viger R. S. and Robaire B.: Expression of 4-ene steroid 5α-reductase messenger ribonuclear acid in the rat epididymis during postnatal development. Endocrinology 131 (1992) 1534-1540.
12. Lowry O. H., Rosebrough N. J., Farr A. L. and Randall R. J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275.
13. Span P. N., Smals A. G. H., Sweep C. G. J. and Benraad Th. J.: Rat steroid 5α-reductase kinetic characteristics: extreme pH-dependency of the type II isozyme in prostate and epididymis homogenates. J. Steroid Biochem. Molec. Biol. 54 (1995) 185-192.
14. Segel I. H.: Enzyme Kinetics. John Wiley & Sons, NY (1975) pp. 214-218.
15. Krieg M., Bartsch W., Thomsen M. and Voigt K. D.: Androgens and estrogens: their interaction with stroma and epithelium of human benign prostatic hyperplasia and normal prostate. J. Steroid Biochem. 19 (1983) 155-161.
16. LeGoff J. M., Martin P. M., Ojasoo T. and Raynaud J. P.: Non-Michaelian behavior of 5α-reductase in human prostate. J. Steroid Biochem. 33 (1989) 155-163.
17. Thigpen A. E., Silver R. I., Guileyardo J. M., Casey M. L., McConnell J. D. and Russell D. W.: Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. J. Clin. Invest. 92 (1993) 903-910.
18. 4-3-ketosteroid 5α-oxidoreductase in the nuclear membrane of the rat ventral prostate. J. Biol. Chem. 247 (1972) 958-967.
19. Enderle-Schmitt U., Völck-Badouin E., Schmitt J. and Aumüller G.: Functional characteristics of nuclear 5α-reductase from rat ventral prostate. J. Steroid Biochem. 25 (1986) 209-217.
20. Enderle-Schmitt U., Neuhaus C. and Aumüller G.: Solubilization of nuclear steroid 5α-reductase from rat ventral prostate. Biochim. Biophys. Acta 987 (1989) 21-28.
21. Fang S., Anderson K. M. and Liao S.: Receptor proteins for androgens: on the role of specific proteins in selective retention of 17/β-hydroxy-5α-androstan-3-one by rat ventral prostate in vivo and in vitro. J. Biol. Chem. 244 (1969) 6584-6595.
22. Mainwaring W. I. P.: A soluble androgen receptor in the cytoplasm of rat prostate. J. Endocr. 45 (1969) 531-541.
23. Monsalve A. and Blaquier J. A.: Partial characterization of epididymal 5α reductase in the rat. Steroids 30 (1977) 41-51.
24. 4-5α-reductase and 3α-hydroxysteroid dehydrogenase in the rat epididymis during sexual maturation. Biol. Reprod. 29 (1983) 1-10.
25. Kawai C. and Ichihara K.: Phospholipid requirement of epididymal testosterone 5α-reductase and phospholipid composition of epididymal microsomes. Steroids 58 (1993) 472-477.