Gram staining applied to human spermatozoa: A simple method for studying chromatin condensation status

Biotechnic and Histochemistry

Volumn 71, Issue 2, 1996, Pages 61-65

  Radicioni, A ^a   Morgia, A M ^a   Paris, E ^a   Dondero, F ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

chromatin; Gram staining; nuclear condensation; spermatozoa

Indexed keywords

ANILINE DERIVATIVE;

ANALYTIC METHOD; ARTICLE; CELL POPULATION; CHROMATIN CONDENSATION; GRAM STAINING; HUMAN; HUMAN CELL; LABORATORY TEST; MALE FERTILITY; PROTEIN ANALYSIS; SPERMATOZOON;

NEGIBACTERIA; POSIBACTERIA;

EID: 0029969380     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520299609117134     Document Type: Article

References (16)

1. Armitage, P. 1971. Statistical Methods in Medical Research. Blackwell, Oxford. pp. 147-268.
2. Auger, J., Mesbah, M., Huber, C. and Dadoune, J. P. 1990. Aniline blue staining as a marker of sperm chromatin defects associated with different semen characteristics discriminates between proven fertile and suspected infertile men. Int. J. Androl. 13: 452-462.
3. Brachet, J. 1946. Embryologie Chimique. Desoer, Liège. p. 37.
4. Dadoune, J. T., Mayaux, M. J. and Guihard-Moscato, M. L. 1988. Correlation between defects in chromatin condensation of human spermatozoa stained by aniline blue and semen characteristics. Andrologia 20: 211-217.
5. Eliasson, R. and Treichl, L. 1971. Supravital staining of human spermatozoa. Fertil. Steril. 22: 134-137.
6. Foster, C. L. and Wilson, R. R. 1973. Gram-positive cells of the human anterior pituitary. Nature, London 167: 528-529.
7. Gopalkrishnan, K., Hinduja, I. N. and Anand Kumar, T. C. 1991. In vitro decondensation of nuclear chromatin of human spermatozoa: assessing fertilizing potential. Arch. Androl. 27: 43-50.
8. Gusse, M., Sautière, P., Bèlaiche, D., Martinage, A., Roux, C., Dadoune, J. P. and Chevaillier, P. 1986. Purification and characterization of nuclear basic protein of human sperm. Biochem. Biophys. Acta. 884: 124-127.
9. Huret, J. L. 1986. Nuclear chromatin decondensation of human sperm: a review. Arch. Androl. 16: 97-109.
10. Jeyendran, R. S., Van der Ven, H. H., Perez-Pealez, M., Crabo, B. G. and Zaneveld, L. J. D. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70: 219-228.
11. Kvist, U., Bjorndahl, L. and Kjellberg, S. 1987. Sperm nuclear zinc, chromatin stability, and male fertility. Scanning Microsc. 1: 1241-1247.
12. Monné, L. 1955. On the nature of the Gram basophilia. Ark. Zool. 26: 559-572.
13. Panijel, J. 1950. Recherches sur la nature et la signification de la protéine Gram du gamète mâle d'Ascaris megalocephala. Biochem. Biophys. Acta. 6: 79-93.
14. Pearse, A. G. E. 1960. Histochemistry of Gram staining. In: Histochemistry, Theoretical and Applied. 2nd ed., Little, Brown, Boston. p. 215.
15. Terquem, A. and Dadoune, J. P. 1983. Aniline blue staining of human spermatozoon chromatin. Evaluation of nuclear maturation. The Sperm Cell André, J., ed. Martinus Nijoff Publishers, The Hague. pp. 249-252.
16. World Health Organization. 1992. WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press, Cambridge. p. 6.