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13344273526
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25
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0026084405
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Judy. M. M.; Matthews, J. L.; Newman, J. T.; Skiles. H. L.; Boriack, R. L.; Sessler, J. L.; Cyr. M.; Maiya, B. G.; Nichol, S. T. Photochem. Photobiol. 1991. 53. 101-107.
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0001127763
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and references therein
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max of 1 resembles that previously proposed for acridine orange. In the case of acridine orange, the monomeric form absorbs at a higher wavelength than the dimer which, in turn, absorbs at higher wavelength than an extensively aggregated form. A similar effect is seen on the surface of polyanions. While we maintain that the underlying mode of DNA recognition for sapphyrin differs from that of acridine orange, we nonetheless recognize that the underlying photophysics for these two system are similar. See: Bradley. D. F.; Wolf. M. K. Proc. Nail. Acad. Sci. U.S.A. 1959. 45. 944-952, and references therein.
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28
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13344264486
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note
-
max, of two or more overlapping absorption bands.
-
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-
29
-
-
13344254350
-
-
note
-
The titrations were conducted in 10 mM bis-Tris buffer at pH 6.1. Details of the titration and curve-fitting procedures can be found in the supporting information.
-
-
-
-
30
-
-
13344264490
-
-
note
-
31P NMR studies could not be conducted with nucleic acids.
-
-
-
-
31
-
-
13344256809
-
-
note
-
lt was necessary to use an organic cosolvent in these studies to avoid problems involving the coprecipitation of sapphyrin and these nucleotides in aqueous solution in the millimolar concentration regime needed for the NMR work
-
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32
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0001081166
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13344251529
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The higher apparent affinity for ssDNA (as opposed to simple phosphates) may reflect the fact that once 1 is complexed to the polyanionic backbone of ssDNA. u is bound to a two-dimensional array of phosphodiester anions. Consequently, because of the high effective concentration of anions present. 1 may have a considerably slower off rate than when bound to a nucleotide monomer in solution.
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13344254346
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In some cases, a small absorption band resembling that seen with ssDNA was seen at ca. 450 nm at very high P/S ratios (>300) of dsDNA. The appearance of this band was found to be dependent on the batch of dsDNA used and likely reflects the fact that double-stranded calf thymus DNA inevitably contains trace amounts of unpaired single strands. As a result, there is likely some hydrophobic interaction, as discussed in the case of ssDNA. with exposed nucleobases
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