Circadian rhythms in cultured mammalian retina

Science

Volumn 272, Issue 5260, 1996, Pages 419-421

  Tosini, Gianluca ^a   Menaker, Michael ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MELATONIN;

ANIMAL TISSUE; ARTICLE; CIRCADIAN RHYTHM; CONTROLLED STUDY; GENE MUTATION; HAMSTER; HORMONE SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; RETINA NEUROEPITHELIAL LAYER; TISSUE CULTURE;

EID: 0029967843     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5260.419     Document Type: Article

References (20)

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3. M. Menaker and S. Wisner, Proc. Natl. Acad. Sci U.S.A. 80, 6119 (1983), J S. Takahashi, N. Murakami, S. S. Nikaido, B. L. Pratt, L M. Robertson, Recent Progr. Horm. Res. 45, 279 (1989); J. Falcon and J. P Collins, Cell Tissue Res. 265, 601 (1991).
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13. We used a modification of the method of M. Rollag and G. Niswender [Endocrinology 98, 482 (1976)]. The lower limits of the assay were 1.8 to 2.5 pg per tube, and the upper limits were 500 pg per tube
14. M R. Ralph and M Menaker, Science 241, 1225 (1988), M. R. Ralph, R. G. Foster, F. C. Davis, M Menaker, ibid. 247, 975 (1990).
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16. M. Menaker and G Tosini, in Sixth Sapporo Symposium on Biological Rhythm, T. Hiroshige and K. Honma, Eds (Hokkaido Univ. Press, Sapporo, Japan, in press).
17. Analysis of the time-series data was performed by an iterative, coupled Fourier transform-nonlinear least squares estimation procedure developed at the National Science Foundation Center for Biological Timing at the University of Virginia [M L. Johnson and S. G. Frasier, Methods Enzymol. 117, 301 (1985), M. Straume, S G. Frasier-Cadoret, M. L. Johnson, in Topics in Fluorescence Spectroscopy Principles, J. R. Lakowicz, Ed. (Plenum, New York, 1991), vol. 2, p. 177] This software is available from the National Science Foundation Center for Biological Timing at the University of Virginia.
18. Analysis of the time-series data was performed by an iterative, coupled Fourier transform-nonlinear least squares estimation procedure developed at the National Science Foundation Center for Biological Timing at the University of Virginia [M L. Johnson and S. G. Frasier, Methods Enzymol. 117, 301 (1985), M. Straume, S G. Frasier-Cadoret, M. L. Johnson, in Topics in Fluorescence Spectroscopy Principles, J. R. Lakowicz, Ed. (Plenum, New York, 1991), vol. 2, p. 177] This software is available from the National Science Foundation Center for Biological Timing at the University of Virginia.
19. A fiber optic light powered by a 150-W tungsten quartz-halogen lamp was used in the experiments in which the cultured retinas were exposed to L:D cycles. The light source was located outside the incubator, and the tip of the fiber-optic probe was inside the incubator about 15 cm from the plate containing the cultures We estimated light intensities at the level of the retinas by placing an irradiance detector of a UDT 350 radiometer-photometer (United Detector Technology, Hawthorne, CA) at the same location as the superfusion chamber.
20. Supported by International Human Frontier Science Program fellowship (LT-145/93) to G.T and NIH grant (HD-13162) to M M We thank M. Straume for the statistical analysis, G Cahill for discussion, and M Rollag for the melatonin antibody.