Autoantibodies to DNA-dependent protein kinase probes for the catalytic subunit

Journal of Clinical Investigation

Volumn 97, Issue 6, 1996, Pages 1417-1421

  Suwa, Akira ^a,b   Hirakata, Michito ^c   Takeda, Yoshihiko ^a   Okano, Yutaka ^d   Mimori, Tsuneyo ^c   Inada, Shinichi ^b   Watanabe, Fumiaki ^e   Teraoka, Hirobumi ^e   Dynan, William S ^f   Hardin, John A ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

^b Toshima ku   (Japan)

^c KEIO UNIVERSITY   (Japan)

^d NIPPON KOKAN HOSPITAL   (Japan)

^e TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^f UNIVERSITY OF COLORADO   (United States)

Author keywords

Antinuclear antibodies; Autoantibodies; Autoimmunity; DNA dependent protein kinase; Ku protein

Indexed keywords

ANTINUCLEAR ANTIBODY; ANTISERUM; AUTOANTIBODY; DNA; MONOCLONAL ANTIBODY; PROTEIN KINASE;

ANTIBODY SPECIFICITY; ARTICLE; AUTOIMMUNITY; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME SUBUNIT; HELA CELL; HUMAN; HUMAN CELL; HUMORAL IMMUNITY; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MAJOR CLINICAL STUDY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RHEUMATIC DISEASE; SERUM;

ANTIGENS, NUCLEAR; AUTOANTIBODIES; DNA HELICASES; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HELA CELLS; HUMANS; NUCLEAR PROTEINS; PROTEIN-SERINE-THREONINE KINASES;

EID: 0029967674     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118562     Document Type: Article

References (47)

1. r heat-shock protein in animal cell extracts EMBO (Eur. MoL Biol. Organ.) J 4:139-145
2. Carter, T.H., I. Vancurova, I. Sun, W. Lou, and S. DeLon. 1990. A DNA-activated protein kinase from HeLa cell nuclei. Mol. Cell. Biol 10 6460-6471.
3. Lees-Miller, S.P , Y.-R. Chen, and C.W. Anderson. 1990 Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53, and the human Ku autoantigen. Mol. Cell. Biol. 10:6472-6481.
4. Anderson, C W., and S.P. Lees-Miller. 1992. The nuclear serine/threonine protein kinase DNA-PK. Critical Reviews in Eukaryotic Gene Expression 2,283-314.
5. Anderson, C.W. 1993. DNA damage and the DNA-activated protein kinase. Trends Biochem. Sci. 18:433-437.
6. Dvir, A., S R Peterson, M.W. Knuth, H. Lu, and W.S. Dynan. 1992. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II, Proc Nad Acad. Sci. USA. 89: 11920-11924.
7. Gottlieb, T.M , and S P Jackson, 1993. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell. 72 1-20.
8. Watanabe F., H. Teraoka, S. Iijima, T. Mimori, and K. Tsukada 1994. Molecular properties, substrate specificity and regulation of DNA-dependent protein kinase from Raji Burkitt's lymphoma cells. Biochim Biophys. Acta 1223:255-260.
9. Hartley, K.S., D Gell, G.C.M. Smith, H. Zhang, N. Divecha, M.A. Connelly, A. Admon, S.P. Lees-Miller, C.W. Anderson, and S.P. Jackson. 1995. DNA-dependent protein kinase catalytic subunit: A relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell. 82:849-856
10. Mimori, T., M. Akizuki, S. Yamagata, S. Inada, S. Yoshida, and M Homma. 1981. Characterization of a high molecular weight acidic nuclear protein recognized by antibodies in sera from patients with polymyositis-sclero-derma overlap. J. Clin. Invest 68:611-620.
11. Reeves, W.H. 1985. Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera J. Exp. Med 161.18-39.
12. Yaneva, M , R. Ochs, D.K. McRorie, S. Zweig, and H. Busch. 1985. Purification of an 86-70 kDa nuclear DNA-associated protein complex. Biochim Biophys. Acta. 841:22-29.
13. Mimori, T., J.A. Hardin, and J.A. Steitz. 1986. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders. J. Biol. Chem. 261:2274-2278.
14. Mimori, T., and J.A. Hardin. 1986. Mechanism of interaction between Ku protein and DNA J. Biol. Chem. 261:10375-10379.
15. Reeves, W.H., and Z.M. Sthoeger. 1989. Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. J. Biol. Chem. 264:5047-5052.
16. Mimori, T., Y. Ohosone, N. Hama, A. Suwa, M. Akizuki, M. Homma, A. J. Griffith, and J.A. Hardin. 1990. Isolation and characterization of cDNA encoding the 80-kDa subunit protein of the human autoantigen Ku (p70/p80) recognized by autoantibodies from patients with scleroderma-polymyositis overlap syndrome. Proc. Natl. Acad. Sci. USA. 87:1777-1781.
17. Griffith, A.J., P.R. Blier, T. Mimori, and J.A Hardin. 1992. Ku polypeptides synthesized in vitro assemble into complexes which recognize ends of double-stranded DNA. J Biol Chem. 267:331-338.
18. Blier, P R , A.J. Griffith, J. Craft, and J A Hardin. 1993. Binding of Ku protein to DNA. J. Biol. Chem. 268:7594-7601.
19. Paillard, S., and F. Strauss 1991 Analysis of the mechanism of interaction of simian Ku protein with DNA. Nucleic Acids Res. 19.5619-5624.
20. Falzon, M., J.W. Fewell, and E.L. Kuff. 1993 EBP-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single- to double-strand transitions in DNA J. Biol. Chem 268:10546-10552.
21. Suwa, A., M. Hirakata, Y. Takeda, S.A Jesch, T. Mimori, and J.A. Hardin. 1994 DNA-dependent protein kinase (Ku prolein-p350 complex) assembles on double stranded DNA. Proc Natl. Acad. Sci. USA. 91:6904-6908.
22. Kuhn. A , T.M. Gottlieb, S.P. Jackson, and I. Grumint. 1995. DNA-dependent protein kinase: a potent inhibitor of transcription by RNA polymerase I. Genes Dev. 9.193-203.
23. Peterson, S.R., S.A. Jesch, T.N. Chamberlin, A. Dvir, S.K. Rabindran, C. Wu, and W.S. Dynan. 1995. Stimulation of the DNA-dependent protein kinase by RNA polymerase II transcriptional activator proteins. J. Biol. Chem. 270.1449-1454.
24. Getts, R.C., and T.D. Stamato, 1994. Absence of Ku-like DNA end-binding activity in the xrs double-stranded DNA repair deficient mutant. J. Biol. Chem. 269:15981-15984.
25. Rathmell, W.K., and G. Chu. 1994. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks Proc. Natl. Acad. Sci. USA 91:7623-7627.
26. Taccioh, G E., T.M. Gottlieb, T. Blunt, A. Priestley, J. Demengeol, R Mizuta, A.R. Lehmann, F.W. Alt, S.P. Jackson, and P.A. Jeggo. 1994. Ku 80, product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science ( Wash. DC) 265:1442-1445.
27. Smider, V , W.K. Rathmell, M.R. Lieber, and G. Chu. 1994. Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA Science (Wash. DC) 266:288-291.
28. Lees-Miller, S.P., R. Godbout, D.W. Chan, M. Weinfeld, R.S. Day III, G M Barron, and J. Allalunis-Turner. 1995. Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line Science (Wash DC) 267:1183-1185.
29. Boubnov, N.V., K.T. Hall, Z. Wills, S.E. Lee, D.M. He, D.M. Benjamin, C. R Pulaski, H. Band, W. Reeves, E.A. Hendrickson, and D.T. Weaver, 1995. Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen. Proc. Natl. Acad. Sci. USA. 92:890-894
30. Kirchgessner, C.U., C.K. Patil, J.W. Evans, C.A. Cuomo, L M. Fried, T Carter, M.A. Oettiinger, and J.M. Brown 1995 DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science (Wash. DC). 267:1178-1183.
31. Blunt, T., N.J. Finnie, G.E. Taccioli, G.C.M. Smith, J. Demengeot, T.M. Gottlieb, R Mizuta, A.J. Varghese, F W. Alt, P.A. Jeggo, and S.P. Jackson. 1995. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell. 80:813-823.
32. Peterson S.R , A. Kurimasa, M Oshimura, W.S. Dynan, E.M. Bradbury, and D.J. Chen. 1995. Loss of the catalytic subunit of the DNA-depcndent protein kinase in DNA double-strand-break-repair mutant mammalian cells. Proc. Natl Acad. Sci. USA 92:3171-3174.
33. Francoeur, A.M., C.L. Peebles, P.T. Gompper, and E.M. Tan. 1986. Identification of Ki (Ku, p70/p80) autoantibodies and analysis of anti-Ki autoantibody reactivity. J Immunol. 136-648-1653.
34. Yaneva, M., and F.C. Arnett 1989. Antibodies against Ku protein in sera from patients with autoimmune diseases. Clin. Exp. Immunol. 76:366-372.
35. Porges, A.J , T NG, and W.H. Reeves. 1990. Antigenic determinants of the Ku (p70/p80) autoantigen are poorly conserved between species. J. Immunol. 145:4222-4228.
36. Tan, E.M., A.S. Cohen, J.F. Fries, A.T. Mashi, D.J. McShane, N F. Rothfielf, J.G. Schaller, N. Talal, and R.J. Winchester. 1982. The 1982 revised criteria for the classification of systemic lupus erythematosus Arth. Rheum 25: 1271-1277.
37. Subcommittee for Scleroderma Criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee. 1980. Preliminary criteria for the classification of systemic sclerosis (scleroderina). Arth. Rheum. 23:581-590.
38. Bohan, A., and J.B. Peter. 1975. Polymyositis and dermatomyositis. N. Engl J Med. 292:344-347.
39. Arnett F.C., S.M. Edworthy, D.A. Block, D.J. McShane, J.F. Fries, N.S. Cooper, L.A. Healey, S.R. Kaplan, M.H. Liang, H.S. Luuthra, T.A. Medsger, Jr., D.M. Mitchell, D.H Neustadt, R.S. Pinals, J.G Schhaller, J.T. Sharp, R.L. Wilder, and G.G. Hunder. 1988. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arth. Rheum. 31: 315-324.
40. Danniels T.E., and J.P. Whitcher. 1994 Association of patterns of labial salivary gland inflammation with keratoconjunctivitis sicca. Arth Rheum. 37: 869-877.
41. Hirakata M., Y. Okano, U Pati, A. Suwa, T.A. Medsger, Jr., J.A. Hardin. and J. Craft. 1993. Identification of autoantibodies to RNA polymerase II.: occurrence in systemic sclerosis and association with autoantibodies to RNA polymerase I and III. J Clin. Invest. 91:2665-2672.
42. Towbin, H., T. Staehelin, and J. Gordon. 1979 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:4350-4354.
43. Hardin, J.A. 1986. The lupus autoantigens and the pathogenesis of systemic lupus erythematosus. Arth Rheum 29.457-460.
44. Tan, E M. 1989. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv. Immunol. 44:93-151.
45. Fatenejad, S , M.J. Mamula, and J. Craft. 1993. Role of intermolecular/ intrastructural B- and T-cell determinants in the diversification of autoantibodies to ribonucleoprotein particles Proc. Natl. Acad. Sci USA. 90:12010-12014.
46. Fatenejad, S., W Brooks, A. Schwartz, and J Craft. 1994. Pattern of anti-small nuclear ribonucleoprotein antibodies in MRL/Mp-Ipr/Ipr mice suggests that the intact U1 snRNP particle is their autoimmune target. J Immunol. 152:5523-5531.
47. Dong, X., K.J Hamilton, M. Satoh, J. Wang, and W.H. Reeves, 1994. Initiation of autoimmunity to the p53 tumor suppresser protein by complexes of p53 and SV40 large T antigen. J Exp. Med 179:1243-1252