Purification, in vitro reassembly, preliminary sequence analysis of epiplasmins, the major constituent of the membrane skeleton of paramecium

Cell Motility and the Cytoskeleton

Volumn 34, Issue 2, 1996, Pages 137-151

  Coffe, Gérard ^a   Caer, Jean Pierre Le ^b   Lima, Oscar ^a   Adoutte, André ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b CNRS   (France)

Author keywords

Electron microscopy; Epiplasm; Filament formation; In vitro assembly; Peptide and dna sequencing

Indexed keywords

PARAMECIUM;

MEMBRANE PROTEIN; PROTOZOAL PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; DNA SEQUENCE; ELECTRON MICROSCOPY; NONHUMAN; PARAMECIUM; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; CYTOSKELETAL PROTEINS; DNA, PROTOZOAN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PARAMECIUM TETRAURELIA; PROTOZOAN PROTEINS; REPETITIVE SEQUENCES, NUCLEIC ACID; SEQUENCE ANALYSIS, DNA; SOLUBILITY; XENOPUS;

EID: 0029967075     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0169(1996)34:2<137::aid-cm5>3.0.co;2-8     Document Type: Article

References (35)

1. Allen, R.D. (1971): Fine structure of membranous and microfibrillar systems in the cortex of Paramecium caudatum. J. Cell Biol. 49:1-20.
2. Baroin-Tourancheau, A., Delgado, P., Perasso, R., and Adoutte, A. (1992): A broad molecular phylogeny of ciliates: Identification of major evolutionary trends and radiations within the phylum. Proc. Natl. Acad. Sci. U.S.A. 89:9764-9768.
3. Collins, T., Baker, R.L., Wilhelm, J.M., and Olmsted, J.B. (1980): A cortical scaffold in the ciliate Tetrahymena. J. Ultrastruct. Res. 70:92-103.
4. Curtenaz, S., and Peck, R.K. (1992): A monoclonal study of protein distribution in the membrane skeleton of the ciliate Pseudomicrothorax. J. Cell Sci. 103:1117-1125.
5. Curtenaz, S., Nahon, P., Iftode, F., and Fleury, A. (1994): Interspecific immunological cross-reactions among cortical proteins of four Ciliates. Eur. J. Protistol. 30:440-450.
6. Dress, V.M., Yi, H., Musal, M.R., and Williams, N.E. (1992): Tetrin polypeptides are colocalized in the cortex of Tetrahymena. J. Struct. Biol. 108:187-194.
7. Dubreuil, R.R., and Bouck, G.B. (1988): Interrelationships among the plasma membrane, the membrane skeleton and surface form in a unicellular flagellate. Protoplasma 143:150-164.
8. Fleury, A., Delgado, P., Iftode, F., and Adoutte, A. (1992): Molecular phylogeny of ciliates: What does it tell us about the evolution of the cytoskeleton and of developmental strategies? Dev. Genet. 13:247-254.
9. Gaboriaud, C., Bissery, V., Benchetrit, T., and Mornon, J.P. (1987): Hydrophobic cluster analysis: An efficient new way to compare and analyse amino acid sequences. FEBS Lett. 224:149-155.
10. Garnier, J., Osguthorpe, D.J., and Robson, B. (1978): Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120:97-120.
11. Graceffa, P. (1989): In-register homodimers of smooth muscle tropomyosin. Biochemistry 28:1282-1287.
12. Hatzfeld, M., and Weber, K. (1990): The coiled coil of in vitro assembled keratin filaments is a heterodimer of type I and II keratins: Use of site-specific mutagenesis and recombinant protein expression. J. Cell Biol. 110:1199-1210.
13. Herrmann, H., Eckelt, A., Brettel, M., Grund, C., and Franke, W.W. (1993): Temperature-sensitive intermediate filament assembly. Alternative structures of Xenopus laevis vimentin in vitro and in vivo. J. Mol. Biol. 234:99-113.
14. Holberton, D., Baker, D.A., and Marshall, J. (1988): Segmented α-helical coiled-coil structure of the protein giardin from the Giardia cytoskeleton. J. Mol. Biol. 204:789-795.
15. Honts, I.E., and Williams, N.B. (1990): Tetrins: Polypeptides that form bundled filaments in Tetrahymena. J. Cell Sci. 96:293-302.
16. Hurme, R., Namork, E., Nurmiaho-Lassila, E.-L., and Rhen, M. (1994): Intermediate filament-like network formed in vitro by a bacterial coiled coil protein. J. Biol. Chem. 269:10675-10682.
17. Huttenlauch, I., Geisler, N., Plessmann, U., Peck, R.K., Weber, K., and Stick, R. (1995): Major epiplasmic proteins of ciliates are articulins: Cloning, recombinant expression, and structural characterization. J. Cell Biol. 130:1401-1412.
18. Jeanmaire-Wolf, R., Clérot, J.C., Nahon, P., Iftode, F., Fleury, A., and Adoutte, A. (1993): Isolation and characterization of monoclonal antibodies to cytoskeletal and membrane proteins of the Paramecium cortex. Eur. J. Protistol. 29:311-333.
19. Jencks, W.P. (1969): Forces in aqueous solution. In: "Catalysis in Chemistry and Enzymology." New York: Mc Graw-Hill, pp. 321-436.
20. Keryer, G., Adoutte, A., Ng, S.F., Cohen, J., Garreau de Loubresse, N., Rossignol, M., Stelly, N., and Beisson, J. (1990): Purification of the surface membrane-cytoskeleton complex (cortex) of Paramecium and identification of several of its protein constituents. Eur. J. Protistol. 25:209-225.
21. Laemmli, U.K. (1970): Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680-685.
22. Marrs, J.A., and Bouck, G.B. (1992): The two major membrane skeletal proteins (articulins) of Euglena gracilis define a novel class of cytoskeletal proteins. J. Cell Biol. 118:1465-1475.
23. McConnell, S.J., and Yaffe, M.P. (1992): Nuclear and mitochondrial inheritance in yeast depends on novel cytoplasmic structures defined by the MDM1 protein. J. Cell Biol. 118:385-395.
24. McConnell, S.J., and Yaffe, M.P. (1993). Intermediate filament formation by a yeast protein essential for organelle inheritance. Science 260:687-689.
25. Melkonian, M., and Viguès, B. (1995): Filamentous cytoskeletal systems in protists. In Brugerolle, G., and Mignot, J.P. (eds.): "Protistological Actualities." Clermont-Ferrand: Université Blaise-Pascal, pp. 136-147.
26. Nahon, P., Coffe, G., Le Guyader, H., Darmanaden-Delorme, J., Jeanmaire-Wolf, R., Clérot, J.C., and Adoutte, A. (1993): Identification of the epiplasmins, a new set of cortical proteins of the membrane cytoskeleton in Paramecium. J. Cell Sci. 104:975-990.
27. Parry, D.A.D., and Steinert, P.M. (1992): Intermediate filament structure. Curr. Opin. Cell. Biol. 4:94-98.
28. Peck, R.K. (1977): The ultrastructure of the somatic cortex of Pseudomicrothorax dubius: Structure and function of the epiplasm in ciliated protozoa. J. Cell Sci. 25:367-385.
29. Peck, R.K., Duborgel, F., Huttenlauch, I., and De Haller, G. (1991): The membrane skeleton of Pseudomicrothorax. I. Isolation, structure and composition. J. Cell Sci. 100:693-706.
30. Perutz, M.F., Staden, R., Moens, L., and De Baere, I. (1993): Polar zippers. Curr. Biol. 3:249-253.
31. Perutz, M.F., Johnson, T., Suzuki, M., and Finch, J.T. (1994): Glutamine repeats as polar zippers: Their possible role in inherited neurovegetative diseases. Proc. Nat. Acad. Sci. U.S.A. 91: 5355-5358.
32. Sonneborn, T.M. (1975): The Paramecium aurelia complex of fourteen sibling species. Trans. Am. Micros. Sec. 94:155-178.
33. Stelly, N., Mauger, J.P., Claret, M., and Adoutte, A. (1991): Cortical alveoli of Paramecium: A vast sub-membraneous calcium storage compartment. J. Cell Biol. 113:103-112.
34. Weber, K., Geisler, N., Plessmann, U., Bremerich, A., Lechtreck, K.-F., and Melkonian, M. (1993): SF-assemblin, the structural protein of the 2-nm filaments from striated microtubule associated fibers of algal flagellar roots, forms a segmented coiled coil. J. Cell Biol. 121:837-845.
35. Williams, N.E., Vaudaux, P.E., and Skriver, L. (1979): Cytoskeletal proteins of the cell surface in Tetrahymena. Identification and localization of major proteins. Exp. Cell Res. 123:311-320.