Critical amino acids in the transcriptional activation domain of the herpesvirus protein VP16 are solvent-exposed in highly mobile protein segments: An intrinsic fluorescence study

Journal of Biological Chemistry

Volumn 271, Issue 9, 1996, Pages 4819-4826

  Shen, Fan ^a   Triezenberg, Steven J ^a   Hensley, Preston ^b   Porter, Denise ^c   Knutson, Jay R ^c  

^a Michigan State University   (United States)

^b GLAXOSMITHKLINE   (United Kingdom)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CHIMERIC PROTEIN; FUNGAL PROTEIN; HIGH MOBILITY GROUP PROTEIN; HYBRID PROTEIN; VIRUS PROTEIN;

ANISOTROPY; ARTICLE; BINDING SITE; FLUORESCENCE; GENETIC TRANSCRIPTION; HERPES VIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TRANSCRIPTION INITIATION;

CESIUM; CHLORIDES; CLONING, MOLECULAR; ESCHERICHIA COLI; FLUORESCENCE POLARIZATION; FUNGAL PROTEINS; HERPES SIMPLEX VIRUS PROTEIN VMW65; KINETICS; MATHEMATICS; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; POINT MUTATION; POTASSIUM IODIDE; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SOLVENTS; SPECTROMETRY, FLUORESCENCE; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRYPTOPHAN;

EID: 0029965276     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.271.9.4819     Document Type: Article

References (61)

1. Zawel, L., and Reinberg, D (1995) Annu. Rev. Biochem. 64, 533-561
2. Nelson, H. (1995) Curr. Opin. Genet. Dev. 5, 180-189
3. Hahn, S. (1993) Cell 72, 481-483
4. Tjian, R., and Maniatis, T. (1994) Cell 77, 5-8
5. Triezenberg, S. J. (1995) Curr. Opin. Genet. Dev. 5, 190-196
6. Hayward, G. S. (1993) Semin. Virol. 4, 239-251
7. O'Hare, P. (1993) Semin. Virol 4, 239-251
8. Walker, S., Hayes, S., and O'Hare, P. (1994) Cell 79, 841-852
9. Wilson, A. C., LaMarco, K., Peterson, M. G., and Herr, W. (1993) Cell 74, 115-125
10. Triezenberg, S. J., Kingsbury, R. C , and McKnight, S. L. (1988) Genes & Dev. 2, 718-729
11. Cousens, D. J., Greaves, R, Goding, C. R., and O'Hare, P. (1989) EMBO J. 8, 2337-2342
12. Sadowski, I., Ma, J., Triezenberg, S., and Ptashne, M. (1988) Nature 335, 563-564
13. Paranjape, S. M., Kamakaka, R. T., and Kadonaga, J. T. (1994) Annu. Rev. Biochem. 63, 265-297
14. Ingles, C. J., Shales, M., Cress, W. D., Triezenberg, S. J , and Greenblatt. J (1991) Nature 351, 588-590
15. Choy, B., and Green, M. R. (1994) Nature 366, 531-536
16. Xiao, H , Pearson, A., Coulombe, B., Truat, R., Zhang, S., Regier, J. L., Triezenberg, S. J., Reinberg, D., Flores, O., Ingles, C. J., and Greenblatt, J. (1994) Mol. Cell. Biol. 14, 7013-7024
17. Goodrich, J. A., Hoey, T., Thut, C. J , Admon, A., and Tjian, R. (1993) Cell 75, 519-530
18. Berger, S. L., Pina, B., Silverman, N , Marcus, G. A , Agapite, J., Regier, J. L , Triezenberg, S J., and Guarente, L. (1992) Cell 70, 251-265
19. Jiang, Y., Triezenberg, S. J., and Gralla, J. D. (1994) J. Biol. Chem. 269, 5505-5508
20. Narayan, S., Widen, S. G., Beard, W. A., and Wilson, S H. (1994) J. Biol. Chem. 269, 12755-12763
21. Yankulov, K., Blau, H , Purton, T., Robert, S., and Bentley, D. L (1994) Cell 77, 749-759
22. Sigler, P. B. (1988) Nature 333, 210-212
23. Cress, W. D., and Triezenberg, S. J. (1991) Science 251, 87-90
24. Ma, J., and Ptashne, M. (1987) Cell 48, 847-853
25. Leuther, K. K., Salmeron, J. M., and Johnston, S A. (1993) Cell 72, 575-585
26. Ptashne, M. (1988) Nature 355, 683-689
27. Regier, J. L.. Shen, F., and Triezenberg, S. J. (1993) Proc. Natl. Acad. Sci. U S. A. 90, 883-887
28. Walker, S., Greaves, R., and O'Hare, P. (1993) Mol. Cell. Biol. 13, 5233-5244
29. O'Hare, P., and Williams, G (1992) Biochemistry 31, 4150-4156
30. Donaldson, L., and Capone, J. P. (1992) J. Biol. Chem. 267, 1411-1414
31. Van Hoy, M., Leuther, K. K., Kodadek, T., and Johnston, S. A. (1993) Cell 72, 587-594
32. Eftink, M. R. (1991) in Methods of Biochemical Analysis, Vol 35 (Suelter, C. H., ed) pp. 127-206, John Wiley and Sons, Inc., New York
33. Chasman, D I., Leatherman, J., Carey, M., Ptashne, M , and Kornberg, R. D. (1989) Mol. Cell Biol. 9, 4746-4749
34. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 488-492
35. Pan, T., and Coleman, J. E. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 3145-3149
36. Berger, S. L., Cress, W. D., Cress, A., Triezenberg, S. J., and Guarente, L. (1990) Cell 61, 1199-1208
37. Gill, S. C., and von Hippel, P. H. (1989) Anal. Biochem. 182, 319-326
38. Badea, M. G., and Brand, L. (1979) Methods Enzymol. 75, 378-425
39. Green, S. M., Knutson, J. R., and Hensley, P. (1990) Biochemistry 29, 9159-9168
40. Knutson, J. R., Walbridge, D. G., and Brand, L. (1982) Biochemistry 21, 4671-4679
41. Knutson, J. R., Beechem, J. M., and Brand, L. (1983) Chem. Phys. Lett. 102, 501-507
42. Ross, J. B. A., Schmidt, C. J., and Brand, L. (1981) Biochemistry 20, 4369-4377
43. Boens, N., Janssens, L. D., Ameloot, M., and DeSchryver, F. C. (1990) in Time-Resolved Laser Spectroscopy in Biochemistry II (Lakowicz, J. R., ed), Society of Photo-optical Instrumentation Engineers, pp. 456-467, Bellmgham, WA
44. Dale, R. E., Chen, L. A., and Brand, L. (1977) J. Biol. Chem 252, 7500-7510
45. Kinosta, K., Kawato, S., and Ikegami. (1977) Biophys. J. 20, 289-305
46. Lipari, G., and Szabo, A. (1980) Biophys. J. 30, 489-506
47. Kraulis, P. J., Raine, A. R. C., Gadhavi, P. L., and Laue, E. D. (1992) Nature 356, 448-450
48. Eftink, M. R., and Ghiron, C. A. (1981) Anal. Biochem. 114, 199-227
49. Ando, T., and Asai, H. (1980) J. Biochem. 88, 255-264
50. Ross, J. B. A., Rousslang, K., and Brand, L. (1981) Biochemistry 20, 4361-4369
51. Tran, C. D., and Beddard, G. S. (1985) Eur. J. Biochem. 13, 59-64
52. Cockle, S. A , and Szabo, A. G. (1981) Photochem. Photobiol. 34, 23-27
53. Dahlman-Wright, K., Baumann, H., McEwan, I. J., Almlof, T., Wright, A. P. H., Gustafsson, J-Å., and Hard, T. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 1699-1703
54. Carey, M., Lin, Y. S., Green, M , and Ptashne, M. (1990) Nature 345, 361-364
55. Emami, E. H., and Carey, M. (1992) EMBO J. 11, 5005-5012
56. Pontius, B W. (1993) Trends Biochem 18, 181-186
57. Pontius, B. W., and Berg, P. (1990) Proc. Natl. Acad. Sci U. S. A. 87, 8403-8407
58. Pontius, B. W., and Berg, P. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 8237-8241
59. Xiao, H., Friesen, J. D., and Lis, J. T. (1994) Mol. Cell. Biol. 14, 7507-7516
60. Xiao, H., Lis, J T., Xiao, H., Greenblatt, J., and Friesen, J. D. (1994) Nucleic Acids Res. 22, 1966-1973
61. Shen, F , Triezenberg, S. J., Hensley, P., Denise, P., and Knutson, J. J. Biol. Chem. 271, 4827-4837