A rapid and simplified technique for analysis of archival formalin-fixed, paraffin-embedded tissue by fluorescence in situ hybridization (FISH)

Anticancer Research

Volumn 16, Issue 5 A, 1996, Pages 2533-2536

  Kopf I ^a   Hanson, C ^a   Delle, U ^a   Verbiene I ^a   Weimarck, A ^a,b  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

d limonene; Fluorescence in situ hybridization (FISH); Genetic techniques; Interphase cytogenetics; Molecular probe; Paraffin embedded; Tumour single nuclear suspension

Indexed keywords

FORMALDEHYDE; PARAFFIN; TRYPSIN;

ARTICLE; CELL NUCLEUS; CELL SUSPENSION; CONTROLLED STUDY; FEMALE; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN TISSUE; MALE; PRIORITY JOURNAL; TECHNIQUE;

CELL NUCLEUS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PARAFFIN EMBEDDING; SPECIMEN HANDLING;

EID: 0029964845     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Emmerich P, Jauch A, Hofmann M-C, Cremer T and Walt H: Methods in Laboratory Investigation. Interphase Cytogenetics in Paraffin Embedded Sections from Human Testicular Germ Cell Tumor Xenografts and in Corresponding Cultured Cells. Lab Invest 61: 235-242, 1989.
2. Walt H, Emmerich P, Cremer T, Hofmann M-C and Bannwart F: Supernumerary Chromosome 1 in Interphase Nuclei of Atypical Germ Cells in Paraffin-Embedded Human Seminiferous Tubules. Lab Invest 61:527-531 1989.
3. Hopman AHN, van Hooren E, van de Kaa CA, Vooijs PGP and Ramaekers FCS: Detection of Numerical Chromosome Aberrations Using in Situ Hybridization in Paraffin Sections of Routinely Processed Bladder Cancers. Mod Pathol 4:503-513, 1991.
4. Kim SY, Lee JS, Ro JY, Gay ML, Hong WK and Hittelman WN: Interphase Cytogenetics in Paraffin Sections of Lung Tumors by Non-Isotopic in Situ Hybridization. Mapping Genotype/Phenotype Heterogeneity. Am J Pathol 142: 307-317, 1993.
5. Micale MA, Sanford JS, Powell IJ, Sakr WA and Wolman SR: Defining the Extent and Nature of Cytogenetic Events in Prostatic Adenocarcinoma: Paraffin FISH vs. Metaphase Analysis. Cancer Genet Cytogenet 69: 7-12, 1993.
6. Stock C, Ambros IM, Mann G, Gadner H, Amann G and Ambras PF: Detection of Ip36 Deletions in Paraffin Sections of Neuroblastoma Tissues. Genes Chromosom Cancer 6: 1-9, 1993.
7. Thompson CT, LeBoit PE, Nederlof PM and Gray JW: Thick-Section Fluorescence in Situ Hybridization on Formalin-Fixed, Paraffin-Embedded Archival Tissue Provides a Histogenetic Profile. Am J Pathol 144: 237-243, 1994.
8. Zitzelsberger H, Szucs S, Weier H-U, Lehmann L, Braselmann H, Enders S, Schilling A, Breul J, Hofler H and Bauchinger M: Numerical Abnormalities, of Chromosome 7 in Human Prostate Cancer Detected by Fluorescence in Situ Hybridization (FISH) on Paraffin-embedded Tissue Sections with Centromere-specific DNA Probes. J Pathol 172: 325-335, 1994.
9. Misra DN, Dickman PS and Yunis EJ: Fluorescence In Situ Hybridization (FISH). Detection of MYCN Oncogene Amplification in Neuroblastoma Using Paraffin-Embedded Tissues. Diagn Mol Pathol 4: 128-135, 1995.
10. Novak AZ, Kokai GK, Popovic VP, Ludoski MD and Jurukovski VA: Interphase cytogenetics on paraffin-embedded sections of ovary for detection of genomic constitution in a patient with Turner's syndrome and chromosomal mosaicism. Hum Genet 95: 293-298, 1995.
11. Segers K, Ramael M, Singh SK, van Daele A, Weyler J and van Marck E: Detection of Numerical Chromosomal Aberrations in Paraffin-embedded Malignant Pleural Mesothelioma by Non-isotopic in Situ Hybridization. J Pathol 175: 219-226, 1995.
12. Hedley DW, Friedlander ML, Taylor IW, Rugg CA and Musgrove EA: Method for Analysis of Cellular DNA Content of Paraffin-embedded Pathological Material Using Flow Cytometry. J Histochem Cytochem 31: 1333-1335, 1983.
13. Schofield DE and Fletcher JA: Trisomy 12 in Pediatric Granulosa-Stromal Cell Tumors. Demonstration by a Modified Method of Fluorescence in Situ Hybridization on Paraffin-embedded Material. Am J Pathol 141: 1265-1269, 1992.
14. Lee W, Han K, Harris CP, Shim S, Kim S and Meisner LF: Use of FISH to Detect Chromosomal Translocations and Deletions. Analysis of Chromosome Rearrangement in Synovial Sarcoma Cells from Paraffin-Embedded Specimens. Am J Pathol 143: 15-19, 1993.
15. Persons DL, Takai K, Gibney DJ, Katzmann JA, Lieber MM and Jenkins RB: Comparison of Fluorescence in Situ Hybridization With Flow Cytometry and Static Image Analysis in Ploidy Analysis of Paraffin-Embedded Prostate Adenocarcinoma. Hum Pathol 25: 678-683, 1994.
16. Wolman SR, Waldman FM and Balázs M: Complementarity of Interphase and Metaphase Chromosome Analysis in Human Renal Tumors. Genes Chromosom Cancer 6: 17-23, 1993.
17. Dhingra K, Sneige N, Pandita TK, Johnston DA, Lee JS, Emami K, Hortobágyi GN and Hittelman WN: Quantitative Analysis of Chromosome in Situ Hybridization Signal in Paraffin-Embedded Tissue Sections. Cytometry 16: 100-112, 1994.
18. Hyytinen E, Visakorpi T, Kallioniemi A, Kallioniemi O-P and Isola JJ: Improved Technique for Analysis of Formalin-Fixed, Paraffin-Embedded Tumors by Fluorescence in Situ Hybridizalion. Cytometry 16: 93-99, 1994.
19. Kuchinka BD, Kalousek DK, Lomax BL, Harrison KJ and Barrett IJ: Interphase Cytogenetic Analysis of Single Cell Suspensions Prepared from Previously Formalin-Fixed and Paraffin-Embedded Tissues. Mod Pathol 8: 183-186, 1995.
20. Schutte B, Reynders MMJ, Bosman FT and Blijham GH: Flow Cytometric Determination of DNA Ploidy Level in Nuclei Isolated From ParaffinEmbedded Tissue. Cytometry 6: 26-30, 1985.
21. Jakobsson A-H, Hanson C, Wikland M and Hamberger L: Fluorescent in Situ hybridization analysis of chromosomally normal gametes and abnormal arrested embryos. J Assist Reprod Genet 12: 422-427, 1995.
22. Vindeløv LL, Christensen IJ and Nissen NI: A Detergent-Trypsin Method for the Preparation of Nuclei for Flow Cytometric DNA Analysis. Cytometry 3: 323-327, 1983.
23. Vindeløv LL: Flow Microfluorometric Analysis of Nuclear DNA in Cells from Solid Tumors and Cell Suspensions. A New Method for Rapid Isolation and Staining of Nuclei. Virchows Arch [B] 24: 227-242, 1977.