Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome

Journal of Bacteriology

Volumn 178, Issue 8, 1996, Pages 2279-2286

  Pagès, Sandrine ^a   Belaich, Anne ^a   Tardif, Chantal ^a,b   Reverbel Leroy, Corinne ^a   Gaudin, Christian ^a   Belaich, Jean Pierre ^a,b  

^a CNRS   (France)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSE; GLUCAN SYNTHASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CLOSTRIDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

CLOSTRIDIUM CELLULOLYTICUM; CLOSTRIDIUM CELLULOVORANS; ESCHERICHIA COLI;

EID: 0029964699     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.8.2279-2286.1996     Document Type: Article

References (39)

1. Bagnara-Tardif, C., C. Gaudin, A. Bélaich, P. Hoest, T. Citard, and J. P. Bélaich. 1992. Sequence analysis of a gene cluster encoding cellulases from Clostridium cellulolyticum. Gene 119:17-28.
2. Bayer, E., and R. Lamed. 1994. The cellulosome - a treasure-trove for biotechnology. Trends Biotechnol. 12:379-386.
3. Bayer, E. A., and M. Wilchek. 1990 Protein biotinylation. Methods Enzymol 184:138-160
4. Beguin, P., and J. P. Aubert. 1994. The biological degradation of cellulose. FEMS Microbiol Rev. 13:25-58
5. Ducros, V., M. Czjzek, A. Bélaich, C. Gaudin, H. P. Fierobe, J. P. Bélaich, G. J. Davies, and R. Haser. 1995. Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5 Structure 3:939-949.
6. Faure, E., A. Bélaich, C. Bagnara, C. Gaudin, and J. P. Bélaich. 1989. Sequence analysis of the Clostridium cellulolyticum endoglucanase-A-encoding gene, celCCA. Gene 84:39-46.
7. Fierobe, H. P., C. Bagnara-Tardif, C. Gaudin, F. Guerlesquin, P. Sauve, A. Belaich, and J. P. Bélaich. 1993. Purification and characterization of endoglucanase C from Clostridium cellulolyticum - catalytic comparison with endoglucanase A Eur. J. Biochem. 217:557-565.
8. Fierobe, H.-P., C. Gaudin, A. Belaich, M. Loutfi, E. Faure, C. Bagnara, D. Baty, and J.-P. Belaich. 1991. Characterization of endoglucanase A from Clostridium cellulolyticum. J. Bacteriol. 173:7956-7962.
9. Foong, F., T. Hamamoto, O. Shoseyov, and R. H. Doi. 1991. Nucleotide sequence and characteristics of endoglucanase gene engB from Clostridium cellulovorans. J. Gen. Microbiol. 137:1729-1736.
10. Fürste, J. P., W. Pansegrau, R. Frank, H. Blöker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986 Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
11. Gal, L., and A. Bélaich. Unpublished data.
12. Gerngross, U. T., M. P. M. Romaniec, T. Kobayashi, N. S. Huskisson, and A. L. Demain. 1993. Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. Mol. Microbiol 8:325-334
13. Giallo, J., C. Gaudin, and J.-P. Belaich. 1985. Metabolism and solubilization of cellulose by Clostridium cellulolyticum H10. Appl. Environ. Microbiol. 49: 1216-1221
14. Giallo, J., C. Gaudin, J. P. Belaich, E. Petitdemange, and F. Caillet-Mangin. 1983. Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp strain H10. Appl. Environ. Microbiol 45:843-849.
15. Goldstein, M. A., M. Takagi, S. Hashida, O. Shoseyov, R. H. Doi, and I. H. Segel. 1993. Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. J Bacteriol. 175:5762-5768.
16. Hamamoto, T., F. Foong, O. Shoseyov, and R. H. Doi. 1992. Analysis of functional domains of endoglucanases from Clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction. Mol. Gen Genet. 231:472-479
17. Henrissat, B., and A. Bairoch. 1993. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293:781-788
18. Kruus, K., A. C. Lua, A. L. Demain, and J. H. D. Wu. 1995. The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. Proc. Natl. Acad. Sci. USA 92:9254-9258.
19. Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
20. Lamed, R., J. Tormo, A. J. Chirino, E. Morag, and E. A. Bayer. 1994. Crystallization and preliminary X-ray analysis of the major cellulose-binding-domain of the cellulosome from Clostridium thermocellum. J. Mol. Biol. 244: 236-237.
21. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y.
22. Morag, E., A. Lapidot, D. Govorko, R. Lamed, M. Wilchek, E. A. Bayer, and Y. Shoham. 1995. Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl. Environ Microbiol. 61:1980-1986.
23. Ochman, H., M. M. Medhora, D. Garza, and D. L. Hartl. 1990 Amplification of flanking sequences by inverse PCR, p. 219-227. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed), PCR protocols. Academic Press, Inc., New York.
24. Petitdemange, E., F. Caillet, J. Giallo, and C. Gaudin. 1984. Clostridium cellulolyticum sp. nov., a cellulolytic mesophilic species from decayed grass. Int. J. Syst. Bacteriol. 34:155-159
25. Poole, D. M., E. Morag, R. Lamed, E. A. Bayer, G. P. Hazlewood, and H. J. Gilbert. 1992 Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS. FEMS Microbiol. Lett. 99:181-186.
26. Quiviger, B., C. Franche, G. Lutfalla, D. Rice, R. Haselkorn, and C. Elmerich. 1982. Cloning of nitrogen fixation (nif) gene cluster of Azospirillum brasilense. Biochimie 64:495-502.
27. Reverbel-Leroy, C., A. Belaich, A. Bernadac, C. Gaudin, J. P. Belaich, and C. Tardif. Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Microbiology, in press.
28. Roig, V., H. P. Fierobe, V. Ducros, M. Czjzek, A. Bélaich, C. Gaudia, J. P. Béiaich, and R. Haser. 1993. Crystallization and preliminary X-ray analysis of the catalytic domain of endoglucanase A from Clostridium cellulolyticum. J. Mol. Biol. 233:325-327.
29. Salamitou, S. 1995 Ph.D. thesis. University of Paris VII, Paris, France.
30. Salamitou, S., O. Raynaud, M. Lemaire, M. Coughlan, P. Béguin, and J.-P. Aubert. 1994. Recognition specificity of the duplicated segments present in Clostridium thermocellum endoglucanase CelD and in the cellulosome-integrating protein CipA. J. Bacteriol. 176:2822-2827.
31. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
32. Shima, S., Y. Igararashi, and T. Kodama. 1991 Nucteotide sequence analysis of the endoglucanase-encoding gene, celCCD, of Clostridium cellulolyticum. Gene 104:33-38.
33. Shoseyov, O., M. Takagi, M. A. Goldstein, and R. H. Doi. 1992. Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. Proc Natl. Acad. Sci. USA 89:3483-3487.
34. Takagi, M., S. Hashida, M. A. Goldstein, and R. H. Doi. 1993. The hydrophobic repeated domain of the Clostridium cellulovorans cellulose binding protein (CbpA) has specific interactions with endoglucanases J. Bacteriol. 175:7119-7122.
35. Tokatlidis, K., P. Dhurjati, and P. Begiun. 1993. Properties conferred on Clostridium thermocellum endoglucanase CelC by grafting the duplicated segment of endoglucanase celD. Protein Eng. 6:793-800.
36. Tokatlidis, K., S. Salamitou, P. Béguin, P. Dhurjati, and J. P. Aubert. 1991. Interaction of the duplicated segment carried by Clostridium thermocellum cellulases with cellulosome components. FEBS Lett. 291:185-188.
37. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
38. Yaron, S., E. Morag, E. A. Bayer, R. Lamed, and Y. Shoham. 1995. Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome. FEBS Lett. 360:121-124.
39. Watson, M. E. E. 1984. Compilation of published signal sequences. Nucleic Acids Res. 12:5145-5164